PDE3A (NM_000921) Human Untagged Clone

CAT#: SC300151

PDE3A (untagged)-Human phosphodiesterase 3A, cGMP-inhibited (PDE3A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE3A
• Synonyms: CGI-PDE; CGI-PDE-A; CGI-PDE A; HTNB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000921 edited
  GGGGGCCACTGGGAATTCAGTGAAGAGGGCACCCTATACCATGGCAGTGCCCGGCGACGC
  TGCACGAGTCAGGGACAAGCCCGTCCACAGTGGGGTGAGTCAAGCCCCCACGGCGGGCCG
  GGACTGCCACCATCGTGCGGACCCCGCATCGCCGCGGGACTCGGGCTGCCGTGGCTGCTG
  GGGAGACCTGGTGCTGCAGCCGCTCCGGAGCTCTCGGAAACTTTCCTCCGCGCTGTGCGC
  GGGCTCCCTGTCCTTTCTGCTGGCGCTGCTGGTGAGGCTGGTCCGCGGGGAGGTCGGCTG
  TGACCTGGAGCAGTGTAAGGAGGCGGCGGCGGCGGAGGAGGAGGAAGCAGCCCCGGGAGC
  AGAAGGGGGCGTCTTCCCGGGGCCTCGGGGAGGTGCTCCCGGGGGCGGTGCGCGGCTCAG
  CCCCTGGCTGCAGCCCTCGGCGCTGCTCTTCAGTCTCCTGTGTGCCTTCTTCTGGATGGG
  CTTGTACCTCCTGCGCGCCGGGGTGCGCCTGCCTCTGGCTGTCGCGCTGCTGGCCGCCTG
  CTGCGGGGGGGAAGCGCTCGTCCAGATTGGGCTGGGCGTCGGGGAGGATCACTTACTCTC
  ACTCCCCGCCGCGGGGGTGGTGCTCAGCTGCTTGGCCGCCGCGACATGGCTGGTGCTGAG
  GCTGAGGCTGGGCGTCCTCATGATCGCCTTGACTAGCGCGGTCAGGACCGTGTCCCTCAT
  TTCCTTAGAGAGGTTCAAGGTCGCCTGGAGACCTTACCTGGCGTACCTGGCCGGCGTGCT
  GGGGATCCTCTTGGCCAGGTACGTGGAACAAATCTTGCCGCAGTCCGCGGAGGCGGCTCC
  AAGGGAGCATTTGGGGTCCCAGCTGATTGCTGGGACCAAGGAAGATATCCCGGTGTTTAA
  GAGGAGGAGGCGGTCCAGCTCCGTCGTGTCCGCCGAGATGTCCGGCTGCAGCAGCAAGTC
  CCATCGGAGGACCTCCCTGCCCTGTATACCGAGGGAACAGCTCATGGGGCATTCAGAATG
  GGACCACAAACGAGGGCCAAGAGGATCACAGTCTTCAGGAACCAGTATTACTGTGGACAT
  CGCCGTCATGGGCGAGGCCCACGGCCTCATTACCGACCTCCTGGCAGACCCTTCTCTTCC
  ACCAAACGTGTGCACATCCTTGAGAGCCGTGAGCAACTTGCTCAGCACACAGCTCACCTT
  CCAGGCCATTCACAAGCCCAGAGTGAATCCCGTTACTTCGCTCAGTGAAAACTATACCTG
  TTCTGACTCTGAAGAGAGCTCTGAAAAAGACAAGCTTGCTATTCCAAAGCGCCTGAGAAG
  GAGTTTGCCTCCTGGCTTGTTGAGACGAGTTTCTTCCACTTGGACCACCACCACCTCGGC
  CACAGGTCTACCCACCTTGGAGCCTGCACCAGTACGGAGAGACCGCAGCACCAGCATCAA
  ACTGCAGGAAGCACCTTCATCCAGTCCTGATTCTTGGAATAATCCAGTGATGATGACCCT
  CACCAAAAGCAGATCCTTTACTTCATCCTATGCTATTTCTGCAGCTAACCATGTAAAGGC
  TAAAAAGCAAAGTCGACCAGGTGCCCTCGCTAAAATTTCACCTCTTTCATCGCCCTGCTC
  CTCACCTCTCCAAGGGACTCCTGCCAGCAGCCTGGTCAGCAAAATTTCTGCAGTGCAGTT
  TCCAGAATCTGCTGACACAACTGCCAAACAAAGCCTAGGTTCTCACAGGGCCTTAACTTA
  CACTCAGAGTGCCCCAGACCTATCCCCTCAAATCCTGACTCCACCTGTTATATGTAGCAG
  CTGTGGCAGACCATATTCCCAAGGGAATCCTGCTGATGAGCCCCTGGAGAGAAGTGGGGT
  AGCCACTCGGACACCAAGTAGAACAGATGACACTGCTCAAGTTACCTCTGATTATGAAAC
  CAATAACAACAGTGACAGCAGTGACATTGTACAGAATGAAGATGAAACAGAGTGCCTGAG
  AGAGCCTCTGAGGAAAGCATCGGCTTGCAGCACCTATGCTCCTGAGACCATGATGTTTCT
  GGACAAACCAATTCTTGCTCCCGAACCTCTTGTCATGGATAACCTGGACTCAATTATGGA
  GCAGCTAAATACTTGGAATTTTCCAATTTTTGATTTAGTGGAAAATATAGGAAGAAAATG
  TGGCCGTATTCTTAGTCAGGTATCTTACAGACTTTTTGAAGACATGGGCCTGTTTGAAGC
  TTTTAAAATTCCAATTAGGGAATTTATGAATTATTTTCATGCTTTGGAGATTGGATATAG
  GGATATTCCTTATCATAACAGAATCCATGCCACTGATGTTTTACATGCTGTTTGGTATCT
  TACTACACAGCCTATTCCAGGCCTCTCAACTGTGATTAATGATCATGGTTCAACCAGTGA
  TTCAGATTCTGACAGTGGATTTACACATGGACATATGGGATATGTATTCTCAAAAACGTA
  TAATGTGACAGATGATAAATACGGATGTCTGTCTGGGAATATCCCTGCCTTGGAGTTGAT
  GGCGCTGTATGTGGCTGCAGCCATGCACGATTATGATCATCCAGGAAGGACTAATGCTTT
  CCTGGTTGCAACTAGTGCTCCTCAGGCGGTGCTATATAACGATCGTTCAGTTTTGGAGAA
  TCATCACGCAGCTGCTGCATGGAATCTTTTCATGTCCCGGCCAGAGTATAACTTCTTAAT
  TAACCTTGACCATGTGGAATTTAAGCATTTCCGTTTCCTTGTCATTGAAGCAATTTTGGC
  CACTGACCTGAAGAAACACTTTGACTTCGTAGCCAAATTTAATGGCAAGGTAAATGATGA
  TGTTGGAATAGATTGGACCAATGAAAATGATCGTCTACTGGTTTGTCAAATGTGTATAAA
  GTTGGCTGATATCAATGGTCCAGCTAAATGTAAAGAACTCCATCTTCAGTGGACAGATGG
  TATTGTCAATGAATTTTATGAACAGGGTGATGAAGAGGCCAGCCTTGGATTACCCATAAG
  CCCCTTCATGGATCGTTCTGCTCCTCAGCTGGCCAACCTTCAGGAATCCTTCATCTCTCA
  CATTGTGGGGCCTCTGTGCAACTCCTATGATTCAGCAGGACTAATGCCTGGAAAATGGGT
  GGAAGACAGCGATGAGTCAGGAGATACTGATGACCCAGAAGAAGAGGAGGAAGAAGCACC
  AGCACCAAATGAAGAGGAAACCTGTGAAAATAATGAATCTCCAAAAAAGAAGACTTTCAA
  AAGGAGAAAAATCTACTGCCAAATAACTCAGCACCTCTTACAGAACCACAAGATGTGGAA
  GAAAGTCATTGAAGAGGAGCAACGGTTGGCAGGCATAGAAAATCAATCCCTGGACCAGAC
  CCCTCAGTCGCACTCTTCAGAACAGATCCAGGCTATCAAGGAAGAAGAAGAAGAGAAAGG
  GAAACCAAGAGGCGAGGAGATACCAACCCAAAAGCCAGACCAGTGACAATGGATAGAATG
  GGCTGTGTTTCCAAACAGATTGACTTGTCAAAGACTCTCTTCAAGCCAGCACAAGCA
  
• Restriction Sites: Please inquire
• ACCN: NM_000921
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000921.3, NP_000912.3
• RefSeq Size: 4124 bp
• RefSeq ORF: 3426 bp
• Locus ID: 5139
• UniProt ID: Q14432
• Cytogenetics: 12p12.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Insulin signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.