IL5RA (NM_000564) Human Untagged Clone

CAT#: SC300097

IL5RA (untagged)-Human interleukin 5 receptor, alpha (IL5RA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL5RA
• Synonyms: CD125; CDw125; HSIL5R3; IL5R
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000564 edited
  CGAATTCGTCGAGAGCGGACGGTTGCTCTGTACAAATCCTCCTCCATATTTGCTTAGAGA
  AAACGTGTTGCCATCCCATCATGAAGGAAGCTGCCTGAGAGTTTTTAACCATTACAGCCG
  TGATGATGAAAGAGTGAAGAACCGCCTCTAAGTTAAAAAGTGCACCCAGAGATAAGGTTC
  GTTCTCAATGCTCTGCCGCTGCTTCTCATCGCATGGCCACCGCATTTCTCAGGCCAGGCA
  CATTGAGCATTGGTCCTGTGCCTGACGCTATGCTAGATGCTGGGGTTGCAGCCACGAGCA
  TAGACACGACAGACACGGTCCTCGCCATCTTCTGTTGAGTACTGGTCGGAACAAGAGGAT
  CGTCTGTAGACAGGATATGATCATCGTGGCGCATGTATTACTCATCCTTTTGGGGGCCAC
  TGAGATACTGCAAGCTGACTTACTTCCTGATGAAAAGATTTCACTTCTCCCGCCTGTCAA
  TTTCACCATTAAAGTTACTGGTTTGGCTCAAGTTCTTTTACAATGGAAACCAAATCCTGA
  TCAAGAGCAAAGGAATGTTAATCTAGAATATCAAGTGAAAATAAACGCTCCAAAAGAAGA
  TGACTATGAAACCAGAATCACTGAAAGCAAATGTGTAACCATCCTCCACAARGGCTTTTC
  AGCAAGTGTGCGGACCATCCTGCAGAACGACCACTCACTACTGGCCAGCAGCTGGGCTTC
  TGCTGAACTTCATGCCCCACCAGGGTCTCCTGGAACCTCAATTGTGAATTTAACTTGCAC
  CACAAACACTACAGAAGACAATTATTCACGTTTAAGGTCATACCAAGTTTCCCTTCACTG
  CACCTGGCTTGTTGGCACAGATGCCCCTGAGGACACGCAGTATTTTCTCTACTATAGGTA
  TGGCTCTTGGACTGAAGAATGCCAAGAATACAGCAAAGACACACTGGGGAGAAATATCGC
  ATGCTGGTTTCCCAGGACTTTTATCCTCAGCAAAGGGCGTGACTGGCTTGCGGTGCTTGT
  TAACGGCTCCAGCAAGCACTCTGCTATCAGGCCCTTTGATCAGCTGTTTGCCCTTCACGC
  CATTGATCAAATAAATCCTCCACTGAATGTCACAGCAGAGATTGAAGGAACTCGTCTCTC
  TATCCAATGGGAGAAACCAGTGTCTGCTTTTCCAATCCATTGCTTTGATTATGAAGTAAA
  AATACACAATACAAGGAATGGATATTTGCAGATAGAAAAATTGATGACCAATGCATTCAT
  CTCAATAATTGATGATCTTTCTAAGTACGATGTTCAAGTGAGAGCAGCAGTGAGCTCCAT
  GTGCAGAGAGGCAGGGCTCTGGAGTGAGTGGAGCCAACCTATTTATGTGGGAAATGATGA
  ACACAAGCCCTTGAGAGAGTGGTTTGTCATTGTGATTATGGCAACCATCTGCTTCATCTT
  GTTAATTCTCTCGCTTATCTGTAAAATATGTCATTTATGGATCAAGTTGTTTCCACCAAT
  TCCAGCACCAAAAAGTAATATCAAAGATCTCTTTGTAACCACTAACTATGAGAAAGCTGG
  GTCCAGTGAGACGGAAATTGAAGTCATCTGTTATATAGAGAAGCCTGGAGTTGAGACCCT
  GGAGGATTCTGTGTTTTGACTGTCACTTTGGCATCCTCTGATGAACTCACACATGCCTCA
  GTGCCTCAGTGAAAAGAACAGGGATGCTGGCTCTAAGGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_000564
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000564.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000564.2, NP_000555.2
• RefSeq Size: 2367 bp
• RefSeq ORF: 1263 bp
• Locus ID: 3568
• UniProt ID: Q01344
• Cytogenetics: 3p26.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
• Gene Summary: The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 4 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.