PTCH1 (NM_000264) Human Untagged Clone

CAT#: SC300040

PTCH1 (untagged)-Human patched 1 (PTCH1), transcript variant 1b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTCH1
• Synonyms: BCNS; NBCCS; PTC; PTC1; PTCH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000264 edited
  CAGCGGCTGGTCTGTCAACCGGAGCCCGAGCCCGAGCAGCCTGCGGCCAGCAGCGTCCTC
  GCAAGCCGAGCGCCCAGGCGCGCCAGGAGCCCGCAGCAGCGGCAGCAGCGCGCCGGGCCG
  CCCGGGAAGCCTCCGTCCCCGCGGCGGCGGCGGCGGCGGCGGCAACATGGCCTCGGCTGG
  TAACGCCGCCGAGCCCCAGGACCGCGGCGGCGGCGGCAGCGGCTGTATCGGTGCCCCGGG
  ACGGCCGGCTGGAGGCGGGAGGCGCAGACGGACGGGGGGGCTGCGCCGTGCTGCCGCGCC
  GGACCGGGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTCGCTCTGGAGCAGAT
  TTCCAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCGAAGTTTCAGAG
  ACTCTTATTTAAACTGGGTTGTTACATTCAAAAAAACTGCGGCAAGTTCTTGGTTGTGGG
  CCTCCTCATATTTGGGGCCTTCGCGGTGGGATTAAAAGCAGCGAACCTCGAGACCAACGT
  GGAGGAGCTGTGGGTGGAAGTTGGAGGACGAGTAAGTCGTGAATTAAATTATACTCGCCA
  GAAGATTGGAGAAGAGGCTATGTTTAATCCTCAACTCATGATACAGACCCCTAAAGAAGA
  AGGTGCTAATGTCCTGACCACAGAAGCGCTCCTACAACACCTGGACTCGGCACTCCAGGC
  CAGCCGTGTCCATGTATACATGTACAACAGGCAGTGGAAATTGGAACATTTGTGTTACAA
  ATCAGGAGAGCTTATCACAGAAACAGGTTACATGGATCAGATAATAGAATATCTTTACCC
  TTGTTTGATTATTACACCTTTGGACTGCTTCTGGGAAGGGGCGAAATTACAGTCTGGGAC
  AGCATACCTCCTAGGTAAACCTCCTTTGCGGTGGACAAACTTCGACCCTTTGGAATTCCT
  GGAAGAGTTAAAGAAAATAAACTATCAAGTGGACAGCTGGGAGGAAATGCTGAATAAGGC
  TGAGGTTGGTCATGGTTACATGGACCGCCCCTGCCTCAATCCGGCCGATCCAGACTGCCC
  CGCCACAGCCCCCAACAAAAATTCAACCAAACCTCTTGATATGGCCCTTGTTTTGAATGG
  TGGATGTCATGGCTTATCCAGAAAGTATATGCACTGGCAGGAGGAGTTGATTGTGGGTGG
  CACAGTCAAGAACAGCACTGGAAAACTCGTCAGCGCCCATGCCCTGCAGACCATGTTCCA
  GTTAATGACTCCCAAGCAAATGTACGAGCACTTCAAGGGGTACGAGTATGTCTCACACAT
  CAACTGGAACGAGGACAAAGCGGCAGCCATCCTGGAGGCCTGGCAGAGGACATATGTGGA
  GGTGGTTCATCAGAGTGTCGCACAGAACTCCACTCAAAAGGTGCTTTCCTTCACCACCAC
  AACCCTGGACGACATCCTGAAATCCTTCTCTGACGTCAGTGTCATCCGCGTGGCCAGCGG
  CTACTTACTCATGCTCGCCTATGCCTGTCTAACCATGCTGCGCTGGGACTGCTCCAAGTC
  CCAGGGTGCCGTGGGGCTGGCTGGCGTCCTGCTGGTTGCACTGTCAGTGGCTGCAGGACT
  GGGCCTGTGCTCATTGATCGGAATTTCCTTTAACGCTGCAACAACTCAGGTTTTGCCATT
  TCTCGCTCTTGGTGTTGGTGTGGATGATGTTTTTCTTCTGGCCCACGCCTTCAGTGAAAC
  AGGACAGAATAAAAGAATCCCTTTTGAGGACAGGACCGGGGAGTGCCTGAAGCGCACAGG
  AGCCAGCGTGGCCCTCACGTCCATCAGCAATGTCACAGCCTTCTTCATGGCCGCGTTAAT
  CCCAATTCCCGCTCTGCGGGCGTTCTCCCTCCAGGCAGCGGTAGTAGTGGTGTTCAATTT
  TGCCATGGTTCTGCTCATTTTTCCTGCAATTCTCAGCATGGATTTATATCGACGCGAGGA
  CAGGAGACTGGATATTTTCTGCTGTTTTACAAGCCCCTGCGTCAGCAGAGTGATTCAGGT
  TGAACCTCAGGCCTACACCGACACACACGACAATACCCGCTACAGCCCCCCACCTCCCTA
  CAGCAGCCACAGCTTTGCCCATGAAACGCAGATTACCATGCAGTCCACTGTCCAGCTCCG
  CACGGAGTACGACCCCCACACGCACGTGTACTACACCACCGCTGAGCCGCGCTCCGAGAT
  CTCTGTGCAGCCCGTCACCGTGACACAGGACACCCTCAGCTGCCAGAGCCCAGAGAGCAC
  CAGCTCCACAAGGGACCTGCTCTCCCAGTTCTCCGACTCCAGCCTCCACTGCCTCGAGCC
  CCCCTGTACGAAGTGGACACTCTCATCTTTTGCTGAGAAGCACTATGCTCCTTTCCTCTT
  GAAACCAAAAGCCAAGGTAGTGGTGATCTTCCTTTTTCTGGGCTTGCTGGGGGTCAGCCT
  TTATGGCACCACCCGAGTGAGAGACGGGCTGGACCTTACGGACATTGTACCTCGGGAAAC
  CAGAGAATATGACTTTATTGCTGCACAATTCAAATACTTTTCTTTCTACAACATGTATAT
  AGTCACCCAGAAAGCAGACTACCCGAATATCCAGCACTTACTTTACGACCTACACAGGAG
  TTTCAGTAACGTGAAGTATGTCATGTTGGAAGAAAACAAACAGCTTCCCAAAATGTGGCT
  GCACTACTTCAGAGACTGGCTTCAGGGACTTCAGGATGCATTTGACAGTGACTGGGAAAC
  CGGGAAAATCATGCCAAACAATTACAAGAATGGATCAGACGATGGAGTCCTTGCCTACAA
  ACTCCTGGTGCAAACCGGCAGCCGCGATAAGCCCATCGACATCAGCCAGTTGACTAAACA
  GCGTCTGGTGGATGCAGATGGCATCATTAATCCCAGCGCTTTCTACATCTACCTGACGGC
  TTGGGTCAGCAACGACCCCGTCGCGTATGCTGCCTCCCAGGCCAACATCCGGCCACACCG
  ACCAGAATGGGTCCACGACAAAGCCGACTACATGCCTGAAACAAGGCTGAGAATCCCGGC
  AGCAGAGCCCATCGAGTATGCCCAGTTCCCTTTCTACCTCAACGGCTTGCGGGACACCTC
  AGACTTTGTGGAGGCAATTGAAAAAGTAAGGACCATCTGCAGCAACTATACGAGCCTGGG
  GCTGTCCAGTTACCCCAACGGCTACCCCTTCCTCTTCTGGGAGCAGTACATCGGCCTCCG
  CCACTGGCTGCTGCTGTTCATCAGCGTGGTGTTGGCCTGCACATTCCTCGTGTGCGCTGT
  CTTCCTTCTGAACCCCTGGACGGCCGGGATCATTGTGATGGTCCTGGCGCTGATGACGGT
  CGAGCTGTTCGGCATGATGGGCCTCATCGGAATCAAGCTCAGTGCCGTGCCCGTGGTCAT
  CCTGATCGCTTCTGTTGGCATAGGAGTGGAGTTCACCGTTCACGTTGCTTTGGCCTTTCT
  GACGGCCATCGGCGACAAGAACCGCAGGGCTGTGCTTGCCCTGGAGCACATGTTTGCACC
  CGTCCTGGATGGCGCCGTGTCCACTCTGCTGGGAGTGCTGATGCTGGCGGGATCTGAGTT
  CGACTTCATTGTCAGGTATTTCTTTGCTGTGCTGGCGATCCTCACCATCCTCGGCGTTCT
  CAATGGGCTGGTTTTGCTTCCCGTGCTTTTGTCTTTCTTTGGACCATATCCTGAGGTGTC
  TCCAGCCAACGGCTTGAACCGCCTGCCCACACCCTCCCCTGAGCCACCCCCCAGCGTGGT
  CCGCTTCGCCATGCCGCCCGGCCACACGCACAGCGGGTCTGATTCCTCCGACTCGGAGTA
  TAGTTCCCAGACGACAGTGTCAGGCCTCAGCGAGGAGCTTCGGCACTACGAGGCCCAGCA
  GGGCGCGGGAGGCCCTGCCCACCAAGTGATCGTGGAAGCCACAGAAAACCCCGTCTTCGC
  CCACTCCACTGTGGTCCATCCCGAATCCAGGCATCACCCACCCTCGAACCCGAGACAGCA
  GCCCCACCTGGACTCAGGGTCCCTGCCTCCCGGACGGCAAGGCCAGCAGCCCCGCAGGGA
  CCCCCCCAGAGAAGGCTTGTGGCCACCCCTCTACAGACCGCGCAGAGACGCTTTTGAAAT
  TTCTACTGAAGGGCATTCTGGCCCTAGCAATAGGGCCCGCTGGGGCCCTCGCGGGGCCCG
  TTCTCACAACCCTCGGAACCCAGCGTCCACTGCCATGGGCAGCTCCGTGCCCGGCTACTG
  CCAGCCCATCACCACTGTGACGGCTTCTGCCTCCGTGACTGTCGCCGTGCACCCGCCGCC
  TGTCCCTGGGCCTGGGCGGAACCCCCGAGGGGGACTCTGCCCAGGCTACCCTGAGACTGA
  CCACGGCCTGTTTGAGGACCCCCACGTGCCTTTCCACGTCCGGTGTGAGAGGAGGGATTC
  GAAGGTGGAAGTCATTGAGCTGCAGGACGTGGAATGCGAGGAGAGGCCCCGGGGAAGCAG
  CTCCAACTGAGGGTGATTAAAATCTGAAGCAAAGAGGCCAAAGATTGGAAACCCCCCACC
  CCCACCTCTTTCCAGAACTGCTTGAAGAGAACTGGTTGGAGTTATGGAAAAGATGCCCTG
  TGCCAGGACAGCAGTTCATTGTTACTGTAACCGATTGTATTATTTTGTTAAATATTTCTA
  TAAATATTTAAGAGATGTACACATGTGTAATATAGGAAGGAAGGATGTAAAGTGGTATGA
  TCTGGGGCTTCTCCACTCCTGCCCCAGAGTGTGGAGGCCACAGTGGGGCCTCTCCGTATT
  TGTGCATTGGGCTCCGTGCCACAACCAAGCTTGTCGACTCTAGATTGCGGCCGCGGTCAT
  AGCTGTTTCCTGAACAGATCCCGGGTGGCAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000264 unedited
  CGTGACATTTGTATACGATCATATAGGCGGCCGCGNATTCAAATCTGGTACCGATATCTG
  CAGAATTCGCCCTTCAGCGGCTGGTCTGTCAACCGGAGCCCGAGCCCGAGCAGCCTGCGG
  CCAGCAGCGTCCTCGCAAGCCGAGCGCCCAGGCGCGCCAGGAGCCCGCAGCAGCGGCAGC
  AGCGCGCCGGGCCGCCCGGGAAGCCTCCGTCCCCGCGGCGGCGGCGGCGGCGGCGGCAAC
  ATGGCCTCGGCTGGTAACGCCGCCGAGCCCCAGGACCGCGGCGGCGGCGGCAGCGGCTGT
  ATCGGTGCCCCGGGACGGCCGGCTGGAGGCGGGAGGCGCAGACGGACGGGGGGGCTGCGC
  CGTGCTGCCGCGCCGGACCGGGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTC
  GCTCTGGAGCAGATTTCCAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGA
  GCGAAGTTTCAGAGACTCTTATTTAAACTGGGTTGTTACATTCAAAAAAACTGCGGCAAG
  TTCTTGGTTGTGGGCCTCCTCATATTTGGGGCCTTCGCGGTGGGATTAAAAGCAGCGAAC
  CTCGAGACCAACGTGGAGGAGCTGTGGGTGGAAATTGGAGGACGAGTAAGTCCTGAATTA
  AAATATACTTGCCAGAAGATTGGAAAAAAGGCTATAGTTAAATCCTTCAACTCATGATAC
  AAAACCCCTAAAAAAAAAGGTGGCAAATGTCCTGGACCAAAAAAGCCGCTCCTTACAAAA
  ACTGGGATTTGGCACTTCAAGGCCAGCCGTGGCCCTGGTTACCTGGTAAACAAGGCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000264 unedited
  ACTATTGGTAGAGGTCGCTCTGCAGGGCCAGGNCGNAGCACTGGGGNAGGGTCACAGGCT
  GCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAG
  CTTGGTTGTGGCACGGAGCCCAATGCACAAATACGGAGAGGCCCCACTGTGGCCTCCACA
  CTCTGGGGCAGGAGTGGAGAAGCCCCAGATCATACCACTTTACATCCTTCCTTCCTATAT
  TACACATGTGTACATCTCTTAAATATTTATAGAAATATTTAACAAAATAATACAATCGGT
  TACAGTAACAATGAACTGCTGTCCTGGCACAGGGCATCTTTTCCATAACTCCAACCAGTT
  CTCTTCAAGCAGTTCTGGAAAGAGGTGGGGGTGGGGGGTTTCCAATCTTTGGCCTCTTTG
  CTTCAGATTTTAATCACCCTCAGTTGGAGCTGCTTCCCCGGGGCCTCTCCTCGCATTCCA
  CGTCCTGCAGCTCAATGACTTCCACCTTCGCCGTGTCCACTCTGCTGGGAGTGCTGATGC
  TGGCGGGATCTGAATTCGACTTCATTGTCAGGTATTTCTTTGCTGTGCTGGCGATCCTCA
  CCATCCTCCGCGTTCTCAATGAGGCTGGTTTTGCTTCCCGTGCTTTTGTCCTTCCTTTGG
  ACCATATCCTGAGGTGTCTCCCGCCCAAGGCTTGAACCGGCTGGCCACACCCCTCCCTTG
  AACCCACCCCCAAGCGTGGTCCCGGTTTAACCATGCCCGGCCCGGCCCCAACGCCACAGC
  GGGTTTTGATCTCCTCCGACTTCGGAATTTAGTTTCCCCAGGAAAAAAGTGTACAGGACC
  TAATCAAAGGAACTTCTTGGAACTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_000264
• Insert Size: 4800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000264.2, NP_000255.1
• RefSeq Size: 6825 bp
• RefSeq ORF: 4344 bp
• Locus ID: 5727
• UniProt ID: Q13635
• Cytogenetics: 9q22.32
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• Gene Summary: This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1b) encodes the longest isoform (L).