CTDSPL (NM_005808) Human Untagged Clone

CAT#: SC128039

CTDSPL (untagged)-Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTDSPL
• Synonyms: C3orf8; HYA22; PSR1; RBSP3; SCP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005808, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACGGCCCGGCCATCATCACCCAGGTGACCAACCCCAAGGAGGACGAGGGCCGGTTGCCGGGCGCGG
  GCGAGAAAGCCTCCCAGTGCAACGTCAGCTTAAAGAAGCAGAGGAGCCGCAGCATCCTTAGCTCCTTCTT
  CTGCTGCTTCCGTGATTACAATGTGGAGGCCCCTCCACCCAGCAGCCCCAGTGTGCTTCCGCCACTGGTG
  GAGGAGAATGGTGGGCTTCAGAAGCCACCAGCTAAGTACCTTCTTCCAGAGGTGACGGTGCTTGACTATG
  GAAAGAAATGTGTGGTCATTGATTTAGATGAAACATTGGTGCACAGTTCGTTTAAGCCTATTAGTAATGC
  TGATTTTATTGTTCCGGTTGAAATCGATGGAACTATACATCAGGTGTATGTGCTGAAGCGGCCACATGTG
  GACGAGTTCCTCCAGAGGATGGGGCAGCTTTTTGAATGTGTGCTCTTTACTGCCAGCTTGGCCAAGTATG
  CAGACCCTGTGGCTGACCTCCTAGACCGCTGGGGTGTGTTCCGGGCCCGGCTCTTCAGAGAATCATGTGT
  TTTTCATCGTGGGAACTACGTGAAGGACCTGAGTCGCCTTGGGCGGGAGCTGAGCAAAGTGATCATTGTT
  GACAATTCCCCTGCCTCATACATCTTCCATCCTGAGAATGCAGTGCCTGTGCAGTCCTGGTTCGATGACA
  TGACGGACACGGAGCTGCTGGACCTCATCCCCTTCTTTGAGGGCCTGAGCCGGGAGGACGACGTGTACAG
  CATGCTGCACAGACTCTGCAATAGGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005808 unedited
  ATTTTGTCATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGGCCGCCGC
  GCCGCGCACCCATGGACGGCCCGGCCATCATCACCCAGGTGACCAACCCCAAGGAGGACG
  AGGGCCGGTTGCCGGGCGCGGGCGAGAAAGCCTCCCAGTGCAACGTCAGCTTAAAGAAGC
  AGAGGAGCCGCAGCATCCTTAGCTCCTTCTTCTGCTGCTTCCGTGATTACAATGTGGAGG
  CCCCTCCACCCAGCAGCCCCAGTGTGCTTCCGCCACTGGTGGAGGAGAATGGTGGGCTTC
  AGAAGCCACCAGCTAAGTACCTTCTTCCAGAGGTGACGGTGCTTGACTATGGAAAGAAAT
  GTGTGGTCATTGATTTAGATGAAACATTGGTGCACAGTTCGTTTAAGCCTATTAGTAATG
  CTGATTTTATTGTTCCGGTTGAAATCGATGGAACTATACATCAGGTGTATGTGCTGAAGC
  GGCCACATGTGGACGAGTTCCTCCAGAGGATGGGGCAGCTTTTTGAATGTGTGCTCTTTA
  CTGCCAGCTTGGCCAAGTATGCAGACCCTGTGGCTGACCTCCTAGACCGTGGGGGTGTGT
  TTCCGGGCCCGGCTCTTCAGAGATCATGTGTTTTTCATCGTGGGAACACGTGAAGGACCT
  GAGTCGCCTTGGGCGGGAGCTGAGCAAAGTGATCATTTGTTGACAATTCCCCTGCCTCAT
  ACATCTTCCATCCTGAGAATGCAGTGCTCTGTGCGNTCCCTGGGTTCCGATGACATGGAC
  GGACACCGGNAGCTGCTGGACCTCATCCCCTTCTTTTTGAGGGCTCGAGCCGGNAGGAAC
  CNACGTGTAACAGCATGCTGCACAGACTCTGCNATAGCTGGATACACGGGGGCCCGGTTG
  GGGGAAATTTCCTCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005808 unedited
  AAAGAACCTGTGGCTAGTTTTATTTTGGTTCGGCAGAGGAATACATTGCAAACCTTTTGC
  ATTGAAATACTCGCCCCTGGTAAACGTGCACATTCCGATATGTAGCATTTCCTAAGTATT
  GCTGGATTGTTTATCACATTCAAATGTCCAGCAGAAAATCCATGACTACCCANACACCAG
  CATCTCCACTACCCGGGGTGGGGGAGGGTGTGTAAGTCTGCAGAGAAGCTCCTGGGAGAA
  TGGAACAGGAACAGACATGATCAAAGTTACAGTACAGTAGGAACAGAGGCAGCCAGCCAA
  TTTTGCACAGTTAACTGCAAGTACGTCAAGCCATTCACTCTTCTCGAACCCTGGCATGCC
  AACTGTCCAGCACAGCACCTTTAAAAGCAGAGGCACAAACGTATGATTGTTTGACCATAG
  GGCTGCGTGGTTCTGGATTTTTTATTAGAAAGCCATCTTTAAAAATACAGGGTTGTAAAA
  TGTTGCCAGGTTACAGACCTACAGGTTTTTTTTTTTAACATATAAAGTTCAAATGCTGCC
  CCCAGACAAGTCAGCGTCCTTAGGGCATCCTCCTGGTCCCCATAGACATCTCATCATAAT
  TGCTCCACGACCATAGAACTTCCTCACCTTTTATTGTGCGCGGCTAGCTTCCCTTTATGC
  AATTGTCAAACGCCTTATTTTCCGACCGCTGGTCGCTGGGCACCACTGTACTCACACCTT
  TCCACTCCTCACCAATATTGTATTCCGCTTTCTTTTCCCCTCTTGGGAGTTGCCCCGGCC
  CTTTGCATCATCTACCTTTGCTCCACACATTATTAATACCATATATCGCCCCGTATCTCT
  ATCCCCCCCCGTCCCCCGCGCCGCACATTCGATTCACTTCACCACGCCCCCCCTAATAAT
  TACCCTCACCGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005808
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005808.2, NP_005799.2
• RefSeq Size: 4422 bp
• RefSeq ORF: 798 bp
• Locus ID: 10217
• UniProt ID: O15194
• Cytogenetics: 3p22.2
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2) compared to isoform 1.