LAR (PTPRF) (NM_130440) Human Untagged Clone

CAT#: SC128009

PTPRF (untagged)-Human protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LAR
• Synonyms: BNAH2; LAR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128009 sequence for NM_130440 edited (data generated by NextGen Sequencing)
  ATGGCCCCTGAGCCAGCCCCAGGGAGGACGATGGTGCCCCTTGTGCCTGCACTGGTGATG
  CTTGGTTTGGTGGCAGGCGCCCATGGTGACAGCAAACCTGTCTTCATTAAAGTCCCTGAG
  GACCAGACTGGGCTGTCAGGAGGGGTAGCCTCCTTCGTGTGCCAAGCTACAGGAGAACCC
  AAGCCGCGCATCACATGGATGAAGAAGGGGAAGAAAGTCAGCTCCCAGCGCTTCGAGGTC
  ATTGAGTTTGATGATGGGGCAGGGTCAGTGCTTCGGATCCAGCCATTGCGGGTGCAGCGA
  GATGAAGCCATCTATGAGTGTACAGCTACTAACAGCCTGGGTGAGATCAACACTAGTGCC
  AAGCTCTCAGTGCTCGAAGAGGAACAGCTGCCCCCTGGGTTCCCTTCCATCGACATGGGG
  CCTCAGCTGAAGGTGGTGGAGAAGGCACGCACAGCCACCATGCTATGTGCCGCAGGCGGA
  AATCCAGACCCTGAGATTTCTTGGTTCAAGGACTTCCTTCCTGTAGACCCTGCCACGAGC
  AACGGCCGCATCAAGCAGCTGCGTTCAGGTGCCTTGCAGATAGAGAGCAGTGAGGAATCC
  GACCAAGGCAAGTACGAGTGTGTGGCGACCAACTCGGCAGGCACACGTTACTCAGCCCCT
  GCGAACCTGTATGTGCGAGTGCGCCGCGTGGCTCCTCGTTTCTCCATCCCTCCCAGCAGC
  CAGGAGGTGATGCCAGGCGGCAGCGTGAACCTGACATGCGTGGCAGTGGGTGCACCCATG
  CCCTACGTGAAGTGGATGATGGGGGCCGAGGAGCTCACCAAGGAGGATGAGATGCCAGTT
  GGCCGCAACGTCCTGGAGCTCAGCAATGTCGTACGCTCTGCCAACTACACCTGTGTGGCC
  ATCTCCTCGCTGGGCATGATCGAGGCCACAGCCCAGGTCACAGTGAAAGCTCTTCCAAAG
  CCTCCGATTGATCTTGTGGTGACAGAGACAACTGCCACCAGTGTCACCCTCACCTGGGAC
  TCTGGGAACTCGGAGCCTGTAACCTACTATGGCATCCAGTACCGCGCAGCGGGCACGGAG
  GGCCCCTTTCAGGAGGTGGATGGTGTGGCCACCACCCGCTACAGCATTGGCGGCCTCAGC
  CCTTTCTCGGAATATGCCTTCCGCGTGCTGGCGGTGAACAGCATCGGGCGAGGGCCGCCC
  AGCGAGGCAGTGCGGGCACGCACGGGAGAACAGGCGCCCTCCAGCCCACCGCGCCGCGTG
  CAGGCACGCATGCTGAGCGCCAGCACCATGCTGGTGCAGTGGGAGCCTCCCGAGGAGCCC
  AACGGCCTGGTGCGGGGATACCGCGTCTACTATACTCCGGACTCCCGCCGCCCCCCGAAC
  GCCTGGCACAAGCACAACACCGACGCGGGGCTCCTCACGACCGTGGGCAGCCTGCTGCCT
  GGCATCACCTACAGCCTGCGCGTGCTTGCCTTCACCGCCGTGGGCGATGGCCCTCCCAGC
  CCCACCATCCAGGTCAAGACGCAGCAGGGAGTGCCTGCCCAGCCCGCGGACTTCCAGGCC
  GAGGTGGAGTCGGACACCAGGATCCAGCTCTCGTGGCTGCTGCCCCCTCAGGAGCGGATC
  ATCATGTATGAACTGGTGTACTGGGCGGCAGAGGACGAAGACCAACAGCACAAGGTGACC
  TTCGACCCAACCTCCTCCTACACACTAGAGGACCTGAAGCCTGACACACTCTACCGCTTC
  CAGCTGGCTGCACGCTCGGATATGGGGGTGGGCGTCTTCACCCCCACCATTGAGGCCCGC
  ACAGCCCAGTCCACCCCCTCCGCCCCTCCCCAGAAGGTGATGTGTGTGAGCATGGGCTCC
  ACCACGGTCCGGGTAAGTTGGGTCCCGCCGCCTGCCGACAGCCGCAACGGCGTTATCACC
  CAGTACTCCGTGGCCTACGAGGCGGTGGACGGCGAGGACCGCGGGCGGCATGTGGTGGAT
  GGCATCAGCCGTGAGCACTCCAGCTGGGACCTGGTGGGCCTGGAGAAGTGGACGGAGTAC
  CGGGTGTGGGTGCGGGCACACACAGACGTGGGCCCCGGCCCCGAGAGCAGCCCGGTGCTG
  GTGCGCACCGATGAGGACGTGCCCAGCGGGCCTCCGCGGAAGGTGGAGGTGGAGCCACTG
  AACTCCACTGCTGTGCATGTCTACTGGAAGCTGCCTGTCCCCAGCAAGCAGCATGGCCAG
  ATCCGCGGCTACCAGGTCACCTACGTGCGGCTGGAGAATGGCGAGCCCCGTGGACTCCCC
  ATCATCCAAGACGTCATGCTAGCCGAGGCCCAGGAAACCACTATCAGCGGCCTGACCCCG
  GAGACCACCTACTCCGTTACTGTTGCTGCCTATACCACCAAGGGGGATGGTGCCCGCAGC
  AAGCCCAAAATTGTCACTACAACAGGTGCAGTCCCAGGCCGGCCCACCATGATGATCAGC
  ACCACGGCCATGAACACTGCGCTGCTCCAGTGGCACCCACCCAAGGAACTGCCTGGCGAG
  CTGCTGGGCTACCGGCTGCAGTACTGCCGGGCCGACGAGGCGCGGCCCAACACCATAGAT
  TTCGGCAAGGATGACCAGCACTTCACAGTCACCGGCCTGCACAAGGGGACCACCTACATC
  TTCCGGCTTGCTGCCAAGAACCGGGCTGGCTTGGGTGAGGAGTTCGAGAAGGAGATCAGG
  ACCCCCGAGGACCTGCCCAGCGGCTTCCCCCAAAACCTGCATGTGACAGGACTGACCACG
  TCTACCACAGAACTGGCCTGGGACCCGCCAGTGCTGGCGGAGAGGAACGGGCGCATCATC
  AGCTACACCGTGGTGTTCCGAGACATCAACAGCCAACAGGAGCTGCAGAACATCACGACA
  GACACCCGCTTTACCCTTACTGGCCTCAAGCCAGACACCACTTACGACATCAAGGTCCGC
  GCATGGACCAGCAAAGGCTCTGGCCCACTCAGCCCCAGCATCCAGTCCCGGACCATGCCG
  GTGGAGCAAGTGTTTGCCAAGAACTTCCGGGTGGCGGCTGCAATGAAGACGTCTGTGCTG
  CTCAGCTGGGAGGTTCCCGACTCCTATAAGTCAGCTGTGCCCTTTAAGATTCTGTACAAT
  GGGCAGAGTGTGGAGGTGGACGGGCACTCGATGCGGAAGCTGATCGCAGACCTGCAGCCC
  AACACAGAGTACTCGTTTGTGCTGATGAACCGTGGCAGCAGCGCAGGGGGCCTGCAGCAC
  CTGGTGTCCATCCGCACAGCCCCCGACCTCCTGCCTCACAAGCCGCTGCCTGCCTCTGCC
  TACATAGAGGACGGCCGCTTCGATCTCTCCATGCCCCATGTGCAAGACCCCTCGCTTGTC
  AGGTGGTTCTACATTGTTGTGGTGCCCATTGACCGTGTGGGCGGGAGCATGCTGACGCCA
  AGGTGGAGCACACCCGAGGAACTGGAGCTGGACGAGCTTCTAGAAGCCATCGAGCAAGGC
  GGAGAGGAGCAGCGGCGGCGGCGGCGGCAGGCAGAACGTCTGAAGCCATATGTGGCTGCT
  CAACTGGATGTGCTCCCGGAGACCTTTACCTTGGGGGACAAGAAGAACTACCGGGGCTTC
  TACAACCGGCCCCTGTCTCCGGACTTGAGCTACCAGTGCTTTGTGCTTGCCTCCTTGAAG
  GAACCCATGGACCAGAAGCGCTATGCCTCCAGCCCCTACTCGGATGAGATCGTGGTCCAG
  GTGACACCAGCCCAGCAGCAGGAGGAGCCGGAGATGCTGTGGGTGACGGGTCCCGTGCTG
  GCAGTCATCCTCATCATCCTCATTGTCATCGCCATCCTCTTGTTCAAAAGGAAAAGGACC
  CACTCTCCGTCCTCTAAGGATGAGCAGTCGATCGGACTGAAGGACTCCTTGCTGGCCCAC
  TCCTCTGACCCTGTGGAGATGCGGAGGCTCAACTACCAGACCCCAGGTATGCGAGACCAC
  CCACCCATCCCCATCACCGACCTGGCGGACAACATCGAGCGCCTCAAAGCCAACGATGGC
  CTCAAGTTCTCCCAGGAGTATGAGTCCATCGACCCTGGACAGCAGTTCACGTGGGAGAAT
  TCAAACCTGGAGGTGAACAAGCCCAAGAACCGCTATGCGAATGTCATCGCCTACGACCAC
  TCTCGAGTCATCCTTACCTCTATCGATGGCGTCCCCGGGAGTGACTACATCAATGCCAAC
  TACATCGATGGCTACCGCAAGCAGAATGCCTACACCGCCACGCAGGGCCCCCTGCCCGAG
  ACCATGGGTGATTTCTGGAGGATGGTGTGGGAACAGCGCACGGCCACTGTGGTCATGATG
  ACACGGCTGGAGGAGAAGTCCCGGGTAAAATGTGATCAGTACTGGCCAGCCCGTGGCACC
  GAGACCTGTGGCCTTATTCAGGTGACCCTGTTGGACACAGTGGAGCTGGCCACATACACT
  GTGCGCACCTTCGCACTCCACAAGAGTGGCTCCAGTGAGAAGCGCGAGCTGCGTCAGTTT
  CAGTTCATGGCCTGGCCAGACCATGGAGTTCCTGAGTACCCAACTCCCATCCTGGCCTTC
  CTACGACGGGTCAAGGCCTGCAACCCCCTAGACGCAGGGCCCATGGTGGTGCACTGCAGC
  GCGGGCGTGGGCCGCACCGGCTGCTTCATCGTGATTGATGCCATGTTGGAGCGGATGAAG
  CACGAGAAGACGGTGGACATCTATGGCCACGTGACCTGCATGCGATCACAGAGGAACTAC
  ATGGTGCAGACGGAGGACCAGTACGTGTTCATCCATGAGGCGCTGCTGGAGGCTGCCACG
  TGCGGCCACACAGAGGTGCCTGCCCGCAACCTGTATGCCCACATCCAGAAGCTGGGCCAA
  GTGCCTCCAGGGGAGAGTGTGACCGCCATGGAGCTCGAGTTCAAGTTGCTGGCCAGCTCC
  AAGGCCCACACGTCCCGCTTCATCAGCGCCAACCTGCCCTGCAACAAGTTCAAGAACCGG
  CTGGTGAACATCATGCCCTACGAATTGACCCGTGTGTGTCTGCAGCCCATCCGTGGTGTG
  GAGGGCTCTGACTACATCAATGCCAGCTTCCTGGATGGTTATAGACAGCAGAAGGCCTAC
  ATAGCTACACAGGGGCCTCTGGCAGAGAGCACCGAGGACTTCTGGCGCATGCTATGGGAG
  CACAATTCCACCATCATCGTCATGCTGACCAAGCTTCGGGAGATGGGCAGGGAGAAATGC
  CACCAGTACTGGCCAGCAGAGCGCTCTGCTCGCTACCAGTACTTTGTTGTTGACCCGATG
  GCTGAGTACAACATGCCCCAGTATATCCTGCGTGAGTTCAAGGTCACGGATGCCCGGGAT
  GGGCAGTCAAGGACAATCCGGCAGTTCCAGTTCACAGACTGGCCAGAGCAGGGCGTGCCC
  AAGACAGGCGAGGGATTCATTGACTTCATCGGGCAGGTGCATAAGACCAAGGAGCAGTTT
  GGACAGGATGGGCCTATCACGGTGCACTGCAGTGCTGGCGTGGGCCGCACCGGGGTGTTC
  ATCACTCTGAGCATCGTCCTGGAGCGCATGCGCTACGAGGGCGTGGTCGACATGTTTCAG
  ACCGTGAAGACCCTGCGTACACAGCGTCCTGCCATGGTGCAGACAGAGGACCAGTATCAG
  CTGTGCTACCGTGCGGCCCTGGAGTACCTCGGCAGCTTTGACCACTATGCAACGTAA
  
  Clone variation with respect to NM_130440.2
  4235 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130440 unedited
  GATCTGGTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGCGGTGGCGG
  CGGCAGAGGCGGCGGCTCCAGCTTCGGCTCCGGCTCGGGCTCGGGCTCCGGCTCCGGCTC
  CGGCTCCGGCTCCAGCTCGGGTGGCGGTGGCGGGAGCGGGACCAGGTGGAGGCGGCGGCG
  GCAGAGGAGTGGGAGCAGCGGCCCTAGCGGCTTGCGGGGGGACATGCGGACCGACGGCCC
  CTGGATAGGCGGAAGGAGTGGAGGCCCTGGTGCCCGGCCCTTGGTGCTGAGTATCCAGCA
  AGAGTGACCGGGGTGAAGAAGCAAAGACTCGGTTGATTGTCCTGGGCTGTGGCTGGCTGT
  GGAGCTAGAGCCCTGGATGGCCCCTGAGCCAGCCCCAGGGAGGACGATGGTGCCCCTTGT
  GCCTGCACTGGTGATGCTTGGTTTGGTGGCAGGCGCCCATGGTGACAGCAAACCTGTCTT
  CATTAAAGTCCCTGAGGACCAGACTGGGCTGTCAGGAGGGGTAGCCTCCTTCGTGTGCCA
  AGCTACAGGAGAACCCAAGCCGCGCATCACATGGATGAAGAAGGGGAAGAAAGTCAGCTC
  CCAGCGCTTCGAGGTCATTGAGTTTGATGATGGGGCAGGGTCAGTGCTTCGGATCCAGCC
  ATTGCGGGTGCAGCGAGATGAAGCCATCTATGAGTGTACAGCTACTAACAGCCTGNGTGA
  GATCAACACTAGTGCCAAGCTCTCAGTGCTCGAAGAGAACAGCTGCCCCCTGGGTTCCCT
  TCCATCGACAT
  
• Restriction Sites: Please inquire
• ACCN: NM_130440
• Insert Size: 7700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130440.1, NP_569707.1
• RefSeq Size: 7691 bp
• RefSeq ORF: 5667 bp
• Locus ID: 5792
• UniProt ID: P10586
• Cytogenetics: 1p34.2
• Domains: Y_phosphatase, ig, PTPc_motif, IGc2, IG, FN3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction, Cell adhesion molecules (CAMs), Insulin signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.