TCPTP (PTPN2) (NM_080423) Human Untagged Clone

CAT#: SC128001

PTPN2 (untagged)-Human protein tyrosine phosphatase, non-receptor type 2 (PTPN2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCPTP
• Synonyms: PTN2; PTPT; TC-PTP; TCELLPTP; TCPTP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128001 sequence for NM_080423 edited (data generated by NextGen Sequencing)
  ATGCCCACCACCATCGAGCGGGAGTTCGAAGAGTTGGATACTCAGCGTCGCTGGCAGCCG
  CTGTACTTGGAAATTCGAAATGAGTCCCATGACTATCCTCATAGAGTGGCCAAGTTTCCA
  GAAAACAGAAATCGAAACAGATACAGAGATGTAAGCCCATATGATCACAGTCGTGTTAAA
  CTGCAAAATGCTGAGAATGATTATATTAATGCCAGTTTAGTTGACATAGAAGAGGCACAA
  AGGAGTTACATCTTAACACAGGGTCCACTTCCTAACACATGCTGCCATTTCTGGCTTATG
  GTTTGGCAGCAGAAGACCAAAGCAGTTGTCATGCTGAACCGCATTGTGGAGAAAGAATCG
  GTTAAATGTGCACAGTACTGGCCAACAGATGACCAAGAGATGCTGTTTAAAGAAACAGGA
  TTCAGTGTGAAGCTCTTGTCAGAAGATGTGAAGTCGTATTATACAGTACATCTACTACAA
  TTAGAAAATATCAATAGTGGTGAAACCAGAACAATATCTCACTTTCATTATACTACCTGG
  CCAGATTTTGGAGTCCCTGAATCACCAGCTTCATTTCTCAATTTCTTGTTTAAAGTGAGA
  GAATCTGGCTCCTTGAACCCTGACCATGGGCCTGCGGTGATCCACTGTAGTGCAGGCATT
  GGGCGCTCTGGCACCTTCTCTCTGGTAGACACTTGTCTTGTTTTGATGGAAAAAGGAGAT
  GATATTAACATAAAACAAGTGTTACTGAACATGAGAAAATACCGAATGGGTCTTATTCAG
  ACCCCAGATCAACTGAGATTCTCATACATGGCTATAATAGAAGGAGCAAAATGTATAAAG
  GGAGATTCTAGTATACAGAAACGATGGAAAGAACTTTCTAAGGAAGACTTATCTCCTGCC
  TTTGATCATTCACCAAACAAAATAATGACTGAAAAATACAATGGGAACAGAATAGGTCTA
  GAAGAAGAAAAACTGACAGGTGACCGATGTACAGGACTTTCCTCTAAAATGCAAGATACA
  ATGGAGGAGAACAGTGAGAGGCCAAGATTGACAGACACCTAA
  
  Clone variation with respect to NM_080423.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080423 unedited
  NTGGGGTCGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCCATTACGGCCGG
  GGGAGCGCGCATGCGCCGCAGCGCCAGCGCTCTCCCCGGATCGTGCGGGGCCTGAGCCTC
  TCCGCCGGCGCAGGCTCTGCTCGCGCCAGCTCGCTCCCGCAGCCATGCCCACCACCATCG
  AGCGGGAGTTCGAAGAGTTGGATACTCAGCGTCGCTGGCAGCCGCTGTACTTGGAAATTC
  GAAATGAGTCCCATGACTATCCTCATAGAGTGGCCAAGTTTCCAGAAAACAGAAATCGAA
  ACAGATACAGAGATGTAAGCCCATATGATCACAGTCGTGTTAAACTGCAAAATGCTGAGA
  ATGATTATATTAATGCCAGTTTAGTTGACATAGAAGAGGCACAAAGGAGTTACATCTTAA
  CACAGGGTCCACTTCCTAACACATGCTGCCATTTCTGGCTTATGGTTTGGCAGCAGAAGA
  CCAAAGCAGTTGTCATGCTGAACCGCATTGTGGAGAAAGAATCGGTTAAATGTGCACAGT
  ACTGGCCAACAGATGACCAAGAGATGCTGTTTAAAGAAACAGGATTCAGTGTGAAGCTCT
  TGTCAGAAGATGTGAAGTCGTATTATACAGTACATCTACTACAATTAGAAAATATCAATA
  GTGGTGAAACCAGAACAATATCTCACTTTCATTATACTACCTGGCCAGATTTTGGAGTCC
  CTGAATCACCAGCTTCATTTCTCAATTTCTTGTTTAAAGTGAGAGAATCTGGCTCCTTGA
  ACCCTGACCATGGGCCTGCGGTGATCCACTGTAGTGCAGGCATTGNGCGCTCTGGCACCT
  TCTCTCTGGTAGACACTTGGTCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_080423
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080423.1, NP_536348.1
• RefSeq Size: 1623 bp
• RefSeq ORF: 1062 bp
• Locus ID: 5771
• UniProt ID: P17706
• Cytogenetics: 18p11.21
• Domains: Y_phosphatase
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011]
  Transcript Variant: This variant (3) contains an alternate 3' region, which includes a part of the C-terminal coding region, when compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus, as compared to isoform 1.