Serine Palmitoyltransferase (SPTLC2) (NM_004863) Human Untagged Clone

CAT#: SC127529

SPTLC2 (untagged)-Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Serine Palmitoyltransferase
• Synonyms: hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127529 sequence for NM_004863 edited (data generated by NextGen Sequencing)
  ATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCACGGTGCGGGCGAATGGCTGCGTG
  GCGAACGGGGAAGTACGGAACGGGTACGTGAGGAGCAGCGCTGCAGCCGCAGCCGCAGCC
  GCCGCCGGCCAGATCCATCATGTTACACAAAATGGAGGACTATATAAAAGACCGTTTAAT
  GAAGCTTTTGAAGAAACACCAATGCTGGTTGCTGTGCTCACGTATGTGGGGTATGGCGTA
  CTCACCCTCTTTGGATATCTTCGAGATTTCTTGAGGTATTGGAGAATTGAAAAGTGTCAC
  CATGCAACAGAAAGAGAAGAACAAAAGGACTTTGTGTCATTGTATCAAGATTTTGAAAAC
  TTTTATACAAGGAATCTGTACATGAGGATAAGAGACAACTGGAATCGGCCAATCTGTAGT
  GTGCCTGGAGCCAGGGTGGACATCATGGAGAGACAGTCTCATGATTATAACTGGTCCTTC
  AAGTATACAGGGAATATAATAAAGGGTGTTATAAACATGGGTTCCTACAACTATCTTGGA
  TTTGCACGGAATACTGGATCATGTCAAGAAGCAGCCGCCAAAGTCCTTGAGGAGTATGGA
  GCTGGAGTGTGCAGTACTCGGCAGGAAATTGGAAACCTGGACAAGCATGAAGAACTAGAG
  GAGCTTGTAGCAAGGTTCTTAGGAGTAGAAGCTGCTATGGCGTATGGCATGGGATTTGCA
  ACGAATTCAATGAACATTCCTGCTCTTGTTGGCAAAGGTTGCCTGATTCTGAGTGATGAA
  CTGAACCATGCATCACTGGTTCTGGGAGCCAGACTGTCAGGAGCAACCATTAGAATCTTC
  AAACACAACAATATGCAAAGCCTAGAGAAGCTATTGAAAGATGCCATTGTTTATGGTCAG
  CCTCGGACACGAAGGCCCTGGAAGAAAATTCTCATCCTTGTGGAAGGAATATATAGCATG
  GAGGGATCTATTGTTCGTCTTCCTGAAGTGATTGCCCTCAAGAAGAAATACAAGGCATAC
  TTGTATCTGGATGAGGCTCACAGCATTGGCGCCCTGGGCCCCACAGGCCGGGGTGTGGTG
  GAGTACTTTGGCCTGGATCCCGAGGATGTGGATGTTATGATGGGAACGTTCACAAAGAGT
  TTTGGTGCTTCTGGAGGATATATTGGAGGCAAGAAGGAGCTGATAGACTACCTGCGAACA
  CATTCTCATAGTGCAGTGTATGCCACGTCATTGTCACCTCCTGTAGTGGAGCAGATCATC
  ACCTCCATGAAGTGCATCATGGGGCAGGATGGCACCAGCCTTGGTAAAGAGTGTGTACAA
  CAGTTAGCTGAAAACACCAGGTATTTCAGGAGACGCCTGAAAGAGATGGGCTTCATCATC
  TATGGAAATGAAGACTCTCCAGTAGTGCCTTTGATGCTCTACATGCCTGCCAAAATTGGC
  GCCTTTGGACGGGAGATGCTGAAGCGGAACATCGGTGTCGTTGTGGTTGGATTTCCTGCC
  ACCCCAATTATTGAGTCCAGAGCCAGGTTTTGCCTGTCAGCAGCTCATACCAAAGAAATA
  CTTGATACTGCTTTAAAGGAGATAGATGAAGTTGGGGACCTATTGCAGCTGAAGTATTCC
  CGTCATCGGTTGGTACCTCTACTGGACAGGCCCTTTGACGAGACGACGTATGAAGAAACA
  GAAGACTGA
  
  Clone variation with respect to NM_004863.3
  786 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004863 unedited
  GCGGCCGCGAATTCGGCACCAGCCGCTTTTTCCCTTCGCTGCGTGGCCTTGGCCGAGACC
  GGTCCTCTGCGGAGAGGGCCCCGCCCTCTGTGAAGGCCCGCCCGGGAATTGGCGGCGGCG
  CTGCAGCCATTTCCGGTTTCGGGGAGGTGGGTGGGGTGCGGAGCGGGACTTGGAGCAGCC
  GCCGCCGCTGCCACCGCCTACAGAGCCTGCCTTGCGCCTGGTGCTGCCAGGAAGATGCGG
  CCGGAGCCCGGAGGCTGCTGCTGCCGCCGCACGGTGCGGGCGAATGGCTGCGTGGCGAAC
  GGGGAAGTCCGGAACGGGTACGTGAGGGAGCAGCGCTGCAGCCGCAGCCGCAGCCGCCGC
  CGGCCAGATCCATTCATGTTACACAAAATGGAGGACTATATAAAAGACCCGTTAATGAAA
  CTTTTGAAAAACACCAATGCTGGTTGCTGTGCTCACGTATGGGGGGTATGGCGTACTCAC
  CCTCTTTGGATATCTTTGAGATTCCTTGAGGTATTGGAAATTGAAAAGTGTCCCCTGGCA
  CAAAAGAGAGACCANAGGGCTTTGGGCATTGATCAAGATTTGAAACTTTATACAGGATCT
  GACTTGAGGTAAAAGACACTGGATCGGCATTTGTAGGGGCCTGGACCAGGGTGGCATATG
  GAAAGAAGTCTATGATTTACTGGCCTTCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004863 unedited
  TCTTGGCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTGAGACAGAGTC
  TCCCTCTGTCGCCAAGGCTGGAGTGCAGTGGTGTGATCTTGGCTCATTGCATCCTCTGCC
  TCTTGGGTTCAAGCAATTCCTGTGCCTCAGCCTCCTGACGTAGCTGGGATTACAGGCATG
  TGCCACCATGCCCGGCTAATTTTTGTAATTTTAGTAGAGATGAGGTTTCGCCATGTTGGC
  CAGACTGTTCTCGAACTCCTGGCCTCAAGCAATCTGCCTGCTTCAGCCTCCCAAAGTGTT
  GGGATTACAGGCATGAGCCACTGCGCCCAGCCATGGGCACACATTTCATGTGGATAAAGT
  TGAGGCGAGAAAAAGACATGTTTTTCTCCACATGGAAGGTATTTTTTAAACTGGTTTAAT
  GTATTAAGATGGCTGCTGGGCACTGAGCATCCACGTGAAGCAAGTAGCAGGGTATCTGTA
  GGTTCTGGGACTGAAAAAAAAAATCCCTGTAAGTTCTTGGCTTAGTAGCCAAAAAATAAT
  ATTTGCTTTTTATACCTCCCCATTCACAACCCCTTTGGGCTAAGCTGATTTAAAAAACAT
  TTTTTTTCTAAATGGGGTCTTGCTGTGTTGCCCAGGCTACGCCTCAATTCCTGGGGTTAA
  GCAATCCTCCGCCCTCAGCCTTTCAGTAACTGGGATTGCAAGCTGCATCCGCACCCAGCT
  CCTAACAATTTTTTTTCCCCCTCTTATTGAACCAGGGGCTCACTTTTTCCACCAAGCCTG
  AAAGGGCAGGGGTGCAATAATTGGTTCACTGCAACCTTTAACTTCCAAGGTTAAAGGGAA
  TTCTCCACACCGGGTGGGGAAATACCGCAGGGCCCCACCATGGCCGGGTAATTTAAAAAA
  AAAATTTTTTAAAAAACAGTGCTATTCTTCCCCGGGTGGCCTAACATTCATAAACTCACG
  AG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004863
• Insert Size: 7180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004863.2, NP_004854.1
• RefSeq Size: 7250 bp
• RefSeq ORF: 1689 bp
• Locus ID: 9517
• UniProt ID: O15270
• Cytogenetics: 14q24.3
• Domains: aminotran_1_2
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• Gene Summary: This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. [provided by RefSeq, Mar 2011]