SGSH (NM_000199) Human Untagged Clone

CAT#: SC127166

SGSH (untagged)-Human N-sulfoglucosamine sulfohydrolase (SGSH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGSH
• Synonyms: HSS; MPS3A; SFMD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127166 sequence for NM_000199 edited (data generated by NextGen Sequencing)
  ATGAGCTGCCCCGTGCCCGCCTGCTGCGCGCTGCTGCTAGTCCTGGGGCTCTGCCGGGCG
  CGTCCCCGGAACGCACTGCTGCTCCTCGCGGATGACGGAGGCTTTGAGAGTGGCGCGTAC
  AACAACAGCGCCATCGCCACCCCGCACCTGGACGCCTTGGCCCGCCGCAGCCTCCTCTTT
  CGCAATGCCTTCACCTCGGTCAGCAGCTGCTCTCCCAGCCGCGCCAGCCTCCTCACTGGC
  CTGCCCCAGCATCAGAATGGGATGTACGGGCTGCACCAGGACGTGCACCACTTCAACTCC
  TTCGACAAGGTGCGGAGCCTGCCGCTGCTGCTCAGCCAAGCTGGTGTGCGCACAGGCATC
  ATCGGGAAGAAGCACGTGGGGCCGGAGACCGTGTACCCGTTTGACTTTGCGTACACGGAG
  GAGAATGGCTCCGTCCTCCAGGTGGGGCGGAACATCACTAGAATTAAGCTGCTCGTCCGG
  AAATTCCTGCAGACTCAGGATGACCGGCCTTTCTTCCTCTACGTCGCCTTCCACGACCCC
  CACCGCTGTGGGCACTCCCAGCCCCAGTACGGAACCTTCTGTGAGAAGTTTGGCAACGGA
  GAGAGCGGCATGGGTCGTATCCCAGACTGGACCCCCCAGGCCTACGACCCACTGGACGTG
  CTGGTGCCTTACTTCGTCCCCAACACCCCGGCAGCCCGAGCCGACCTGGCCGCTCAGTAC
  ACCACCGTCGGCCGCATGGACCAAGGAGTTGGACTGGTGCTCCAGGAGCTGCGTGACGCC
  GGTGTCCTGAACGACACACTGGTGATCTTCACGTCCGACAACGGGATCCCCTTCCCCAGC
  GGCAGGACCAACCTGTACTGGCCGGGCACTGCTGAACCCTTACTGGTGTCATCCCCGGAG
  CACCCAAAACGCTGGGGCCAAGTCAGCGAGGCCTACGTGAGCCTCCTAGACCTCACGCCC
  ACCATCTTGGATTGGTTCTCGATCCCGTACCCCAGCTACGCCATCTTTGGCTCGAAGACC
  ATCCACCTCACTGGCCGGTCCCTCCTGCCGGCGCTGGAGGCCGAGCCCCTCTGGGCCACC
  GTCTTTGGCAGCCAGAGCCACCACGAGGTCACCATGTCCTACCCCATGCGCTCCGTGCAG
  CACCGGCACTTCCGCCTCGTGCACAACCTCAACTTCAAGATGCCCTTTCCCATCGACCAG
  GACTTCTACGTCTCACCCACCTTCCAGGACCTCCTGAACCGCACCACAGCTGGTCAGCCC
  ACGGGCTGGTACAAGGACCTCCGTCATTACTACTACCGGGCGCGCTGGGAGCTCTACGAC
  CGGAGCCGGGACCCCCACGAGACCCAGAACCTGGCCACCGACCCGCGCTTTGCTCAGCTT
  CTGGAGATGCTTCGGGACCAGCTGGCCAAGTGGCAGTGGGAGACCCACGACCCCTGGGTG
  TGCGCCCCCGACGGCGTCCTGGAGGAGAAGCTCTCTCCCCAGTGCCAGCCCCTCCACAAT
  GAGCTGTGA
  
  Clone variation with respect to NM_000199.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000199 unedited
  ACGACTTCCTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAGCCGGTTCCGGATCCCGA
  TCCCGAGTCCGAGCCGCCGCCGCCATGAGCTGCCCCGTGCCCGCCTGCTGCGCGCTGCTG
  CTAGTCCTGGGGCTCTGCCGGGCGCGTCCCCGGAACGCACTGCTGCTCCTCGCGGATGAC
  GGAGGCTTTGAGAGTGGCGCGTACAACAACAGCGCCATCGCCACCCCGCACCTGGACGCC
  TTGGCCCGCCGCAGCCTCCTCTTTCGCAATGCCTTCACCTCGGTCAGCAGCTGCTCTCCC
  AGCCGCGCCAGCCTCCTCACTGGCCTGCCCCAGCATCAGAATGGGATGTACGGGCTGCAC
  CAGGACGTGCACCACTTCAACTCCTTCGACAAGGTGCGGAGCCTGCCGCTGCTGCTCAGC
  CAAGCTGGTGTGCGCACAGGCATCATCGGGAAGAAGCACGTGGGGCCGGAGACCGTGTAC
  CCGTTTGACTTTGCGTACACGGAGGAGAATGGCTCCGTCCTCCAGGTGGGGCGGAACATC
  ACTAGAATTAAGCTGCTCGTCCGGAAATTCCTGCAGACTCAGGATGACCGGCCTTTCTTC
  CTCTACGTCGCCTTCCACGACCCCCACCGCTGTGGGCACTCCCAGCCCCAGTACGGAACC
  TTCTGTGAGAAGTTTGGCAACGGAGAGAGCGGCATGGGTCGTATCCCAGACTGGACCCCC
  CAGGCCTACGACCCACTGGACGTGCTGGTGCCTTACTTCGTCCCCACACCCGGCAGCCCG
  AGCCGACCTGGCCGCTCAGTCACCACCGTCGGCCGATGGACCAANGAGTGNACTGGTGCT
  NCAGAGCTGGTGACGCCCGTGTCCTGACGACCACTGGTGATCTTACGTCCGACACGGGAT
  CCCTTCCCAGCGGAGACCAACTGACTGGNCGCCCTGCTGACCTTACTGTTGCTNCCCGAG
  CACCATAGCTGGGCAGTACGAGGCCACTGACTCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000199 unedited
  TATGGACCGCGNGCCGCAATCTAGAGTCGAGTTTTTNCCTTTTTTTTTTTTTTTTTTTTT
  TTTAAACTTCTGTATATTTTACTAAAAAAAAACTTTTACAATAGCTGGCCTGGGGCCTCC
  TCCAGCCCACCCCAGTATGGAGTGATAGGGAGGGAAGGGCAAGGGGAGCCCACCTACTCA
  AGGAAGCGCCCTCTCCTTCGAGGGGTGTCCAGGCCGAGGGGTGTCCAGGCCGGGCTTCTG
  CTCCCGAGGTGGGTGGAGGCAGGGCAGGAACGGCACATTCTCCCAGCAACGCCGACGTCA
  TCCAAGAATTAACCCAAGCCAGAGGACGGGCATCGCCACCCAGCAACGCCAGTGTCACCG
  AAAAATTAGCCCAAGCCAGAGGACGGGCATTGCCACCCAGCAACGCCAGTGTCACCGAAG
  AATTAACCCAAGCCAGAGGACGGGCATCACCACCCAGCAATGCCAGTGTCACCGAAGAAT
  TAACCCAAGCCAGAGGACGGGCATCGCCATGCCTTCATCTTCGGACACTCTCAAAAAAGA
  TAAGCTTCTGCCCAGAAACACCACAGGTTCAAGCTCATCACTCAGGGAGCCTGCTGCCAA
  TCAGCTGAAACCTGCCTGGGGAACAGGGACTTGACCATGGGGCAAAACAGAAGAAGCAGA
  AACCTGCCCAAAGGTCGCTCAAAGGCTTCCTCTAGGCCAGACGCTGGTAAGAGCCACGGC
  GCCGTGCTCCCAGGTGAGTGTCGGTGGGACCTGCGTACTGCCCCAGCGGAACCGGAAGCC
  CCTGCCTGCTCTTTGGGAAGGTTCAGAAGTATCTGTGGCTGCCCAAGCCTGAAGAGGCCT
  AAGGCCTCCATTTGAAGTCCCCAACCAAGCAAGAACAAGACTCCCTGAATGGGCTGGGGC
  CTGCCTGTCCGAAACACTTTGTTAAAATTCCCGGCTGGAATGGTTACACCAGAACAATGG
  CTCCTGCATGAAGAAGCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000199
• Insert Size: 2880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000199.2, NP_000190.1
• RefSeq Size: 2740 bp
• RefSeq ORF: 1509 bp
• Locus ID: 6448
• UniProt ID: P51688
• Cytogenetics: 17q25.3
• Domains: Sulfatase
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: This gene encodes the enzyme sulfamidase; one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with the lysosomal storage disease mucopolysaccaridosis IIIA, also known as Sanfilippo syndrome A, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq, Jun 2017]