PMPCB (NM_004279) Human Untagged Clone

CAT#: SC127012

PMPCB (untagged)-Human peptidase (mitochondrial processing) beta (PMPCB), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMPCB
• Synonyms: Beta-MPP; MAS1; MPP11; MPPB; MPPP52; P-52
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004279, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCTGCGGCGGCTCGAGTGGTGTTGTCATCCGCGGCGCGGCGGCGGCTCTGGGGTTTCAGCGAGA
  GTCTTCTAATCCGAGGCGCTGCGGGACGGTCATTATATTTTGGAGAGAACAGATTAAGAAGTACACAGGC
  TGCTACCCAAGTTGTTCTGAATGTTCCTGAAACAAGAGTAACATGTTTAGAAAGTGGACTCAGAGTAGCT
  TCGGAAGACTCTGGGCTCTCAACATGCACAGTTGGACTCTGGATTGATGCTGGAAGTAGATACGAAAATG
  AGAAGAACAATGGAACAGCACACTTTCTGGAGCATATGGCTTTCAAGGGCACCAAGAAGAGATCCCAGTT
  AGATCTGGAACTTGAGATTGAAAATATGGGTGCTCATCTCAATGCCTATACCTCCAGAGAGCAGACTGTA
  TACTATGCCAAAGCATTCTCTAAAGACTTGCCAAGAGCTGTAGAAATTCTTGCTGATATAATACAAAACA
  GCACATTGGGAGAAGCAGAGATTGAACGTGAGCGTGGAGTAATCCTTAGAGAGATGCAGGAAGTTGAAAC
  CAATTTACAAGAAGTTGTTTTTGATTATCTTCATGCCACAGCTTATCAAAATACTGCACTTGGACGGACA
  ATTTTGGGACCAACTGAAAATATCAAATCTATAAGTCGTAAGGACTTAGTGGATTATATAACCACACATT
  ATAAGGGGCCAAGAATAGTGCTTGCTGCTGCTGGAGGTGTTTCCCATGATGAATTGCTTGACTTAGCAAA
  GTTTCATTTCGGTGACTCTTTATGCACACACAAAGGAGAAATACCAGCTCTGCCTCCCTGCAAATTCACA
  GGAAGTGAGATTCGTGTGAGGGATGACAAGATGCCTTTGGCGCACCTTGCAATAGCTGTTGAAGCTGTTG
  GTTGGGCACATCCAGATACAATCTGTCTCATGGTTGCAAACACGCTGATTGGCAACTGGGATCGCTCTTT
  TGGGGGAGGAATGAATTTATCTAGCAAGCTGGCCCAGCTCACTTGTCATGGCAATCTTTGCCATAGCTTT
  CAGTCTTTCAACACTTCCTACACAGATACAGGATTATGGGGACTGTATATGGTTTGTGAATCATCCACTG
  TTGCAGACATGCTACATGTTGTTCAAAAAGAATGGATGCGACTCTGTACAAGTGTCACAGAAAGTGAGGT
  TGCACGAGCCAGAAATCTTCTGAAAACAAACATGTTGTTGCAGCTTGATGGTTCAACTCCAATTTGTGAA
  GATATTGGTAGGCAAATGTTATGCTATAATAGAAGGATTCCCATCCCTGAGCTTGAAGCAAGAATTGATG
  CTGTGAATGCTGAGACAATTCGAGAAGTATGTACCAAATACATTTATAATAGGAGTCCAGCTATTGCTGC
  TGTTGGTCCCATTAAGCAACTACCAGATTTTAAACAGATACGCAGTAACATGTGTTGGCTTCGTGATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004279 unedited
  ATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGGCGGCTGCGG
  CGGCTCGAGTGGTGTTGTCATCCGCGGCGCGGCGGCGGCTCTGGGGTTTCAGCGAGAGTC
  TTCTAATCCGAGGCGCTGCGGGACGGTCATTATATTTTGGAGAGAACAGATTAAGAAGTA
  CACAGGCTGCTACCCAAGTTGTTCTGAATGTTCCTGAAACAAGAGTAACATGTTTAGAAA
  GTGGACTCAGAGTAGCTTCGGAAGACTCTGGGCTCTCAACATGCACAGTTGGACTCTGGA
  TTGATGCTGGAAGTAGATACGAAAATGAGAAGAACAATGGAACAGCACACTTTCTGGAGC
  ATATGGCTTTCAAGGGCACCAAGAAGAGATCCCAGTTAGATCTGGAACTTGAGATTGAAA
  ATATGGGTGCTCATCTCAATGCCTATACCTCCAGAGAGCAGACTGTATACTATGCCAAAG
  CATTCTCTAAAGACTTGCCAAGAGCTGTAGAAATTCTTGCTGATATAATACAAAACAGCA
  CATTGGGAGAAGCAGAGATTGAACGTGAGCGTGGAGTAATCCTTAGAGAGATGCAGGAAG
  TTGAAACCAATTTACAAGAAGTTGTTTTTGATTATCTTCATGCCACAGCTTATCAAAATA
  CTGCACTTGGACGGACAATTTTGGGACCAACTGAAAATATCAAATCTATAAGTCGTAAGG
  ACTTAGTGGATTATATAACCACACATTATAGAGGNGCCAGAATAGTGCTTGCTGCTGCTG
  GAGGTGTTTTCCATGATGAATTGCTTGACTTAGCAAAGTTTCATTTCCGGTGACTCTTTA
  TGCCCACACAAAGGAGAATACCAGCTCTGCCTCCCTGACAATTCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004279 unedited
  TTTGTACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTATATACATG
  TTCATTTTTATTTTTCTCTAGCTCTGTTTTATAAATACATGTGTTCAAACAATCTTGATT
  AGGAGCATTTTAATCACGAAGCCAACACATGTTACTGCGTATCTGTTTAAAATCTGGTAG
  TTGCTTAATGGGACCAACAGCAGCAATAGCTGTTCTCCTATTATAAATGTATTTGGTACA
  TACTTCTCGAATTGTCTCAGCATTCACAGCATCAATTCTTGCTTCAAGCTCAGGGATGGG
  AATCCTTCTATTATAGCATAACATTTGCCTACCAATATCTTCACAAATTGGAGTTGAACC
  ATCAAGCTGCAACAACATGTTTGTTTTCAGAAGATTTCTGGCTCGTGCAACCTCACTTTC
  TGTGACACTTGTACAGAGTCGCATCCATTCTTTTTGAACAACATGTAGCATGTCTGCAAC
  AGTGGATGATTCACAAACCATATACAGTCCCCATAATCCTGTATCTGTGTAGGAAGTGTT
  GAAAGACTGAAAGCTATGGCAAAGATTGCCATGACAAGTGAGCTGGGCCAGCTTGCTAGA
  TAATTCATTCCTCCCCCAAAAGAGCGATCCCAGTTGCCAATCAGCGTGGTTGCAACCATG
  AGACAGAATGTATCTGGATGTGCCCAACCAACAGCTTCAACAGCTATTGCAAGGGTGCGC
  CAAAAGCAATCTTGTCATCCCTTCAAACGAATCTTAATTTCTGGGAAATTTGCAGGGAGG
  CCAAACTGGTTATTTCTCTTTTGGGGGGGCAAAAAGAGTCCCCCAAATGGAACTTTGCTT
  AGTTAAACCATTTTATCTGGGGAAACCCTCCCGGCAGCAGCAGGCCCTTTTCTGTGCCCC
  TTTAAAAGGGTGGGTTAAAAAAACCACCAATTCCTTCCACACTAAAAATTTAAATATTTC
  AGTTGGGCCCAAATTGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004279
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004279.1, NP_004270.1
• RefSeq Size: 1771 bp
• RefSeq ORF: 1470 bp
• Locus ID: 9512
• UniProt ID: O75439
• Cytogenetics: 7q22.1
• Domains: Peptidase_M16, Peptidase_M16_C
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex. [provided by RefSeq, Jul 2008]