PKI alpha (PKIA) (NM_006823) Human Untagged Clone

CAT#: SC126968

PKIA (untagged)-Human protein kinase (cAMP-dependent, catalytic) inhibitor alpha (PKIA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKI alpha
• Synonyms: PRKACN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126968 sequence for NM_006823 edited (data generated by NextGen Sequencing)
  ATGACTGATGTGGAAACTACATATGCAGATTTTATTGCTTCAGGAAGAACAGGTAGAAGA
  AATGCAATACATGATATCCTGGTTTCCTCTGCAAGTGGCAACAGCAATGAATTAGCCTTG
  AAATTAGCAGGTCTTGATATCAACAAGACAGAAGGTGAAGAAGATGCACAACGAAGTTCT
  ACAGAACAAAGTGGGGAAGCCCAGGGAGAAGCAGCAAAATCTGAAAGCTAA
  
  Clone variation with respect to NM_006823.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006823 unedited
  TATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAGCGGAGGCTGCTGCTGG
  CAGGTGGGGCGCGGGCCGGCGCGAGCTGACCGAGCACTCGGCGGGCGCGGCGGGACTGCG
  GCCCGTGGCGGCGTGCGCGGGGACCTGCGCTGACTAGGTCCGGGGAAGTTTCCTGACTTT
  CTGAGAAGCCCTGGTTTCCCCAAAGAAGTGATTTCTGATAGAAATCTGAAGGTCATCTCC
  AAGAAAAAAGAGATCTAGTATAGTCAATGAATTAAAGACAAGAAGGTTTCCAATCAGTCC
  CTGCTATGTGGATATTTGGTAGCAATGACTGATGTGGAAACTACATATGCAGATTTTATT
  GCTTCAGGAAGAACAGGTAGAAGAAATGCAATACATGATATCCTGGTTTCCTCTGCAAGT
  GGCAACAGCAATGAATTAGCCTTGAAATTAGCAGGTCTTGATATCAACAAGACAGAAGGT
  GAAGAAGATGCACAACGAAGTTCTACAGAACAAAGTGGGGAAGCCCAGGGAGAAGCAGCA
  AAATCTGAAAGCTAACACCCCACTTTGACCCTCGACCACACCTGANAATGTCTCANATCT
  CCAGGAGTATCTGGAATGCATTTTGTTTCCATGAGTGAAAAGAGGAAAAAGAAAATGGGC
  TGTGCTGCATTGCANGAACCTGCTCATTATCATGTNTAAAAATGAGGGCAGAGGCTGTGG
  CTGCAGGCAGACTTTTTCCCTTACCTCTGTCATTAGCCNATGGNTGAAAATCATGTGGCT
  TTGGTGGTTTGGGCGTCAATTTTGGATGGGATCCTTTCACCTNGTCATATGACCAAATGC
  TTATAGAGAGTAGCCTCGACCTAGAGGAAGAATCTTTCTGTAGCATCTTGGCCCTTACAT
  GTCCNAGGAATTAATTGGGGCAATTTCGCAGAGGGTGTTTGAACCCCGAGTTAAATTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006823
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006823.2, NP_006814.1
• RefSeq Size: 2184 bp
• RefSeq ORF: 231 bp
• Locus ID: 5569
• UniProt ID: P61925
• Cytogenetics: 8q21.13
• Domains: PKI
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of the cAMP-dependent protein kinase (PKA) inhibitor family. This protein was demonstrated to interact with and inhibit the activities of both C alpha and C beta catalytic subunits of the PKA. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs the in the 5' UTR, as compared to variant 2. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.