ACSL3 (NM_203372) Human Untagged Clone

CAT#: SC126917

ACSL3 (untagged)-Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACSL3
• Synonyms: ACS3; FACL3; LACS 3; LACS3; PRO2194
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126917 sequence for NM_203372 edited (data generated by NextGen Sequencing)
  ATGAATAACCACGTGTCTTCAAAACCATCTACCATGAAGCTAAAACATACCATCAACCCT
  ATTCTTTTATATTTTATACATTTTCTAATATCACTTTATACTATTTTAACATACATTCCG
  TTTTATTTTTTCTCCGAGTCAAGACAAGAAAAATCAAACCGAATTAAAGCAAAGCCTGTA
  AATTCAAAACCTGATTCTGCATACAGATCTGTTAATAGTTTGGATGGTTTGGCTTCAGTA
  TTATACCCTGGATGTGATACTTTAGATAAAGTTTTTACATATGCAAAAAACAAATTTAAG
  AACAAAAGACTCTTGGGAACACGTGAAGTTTTAAATGAGGAAGATGAAGTACAACCAAAT
  GGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTATAATTGGCTTTCCTATGAAGATGTC
  TTTGTTCGAGCCTTTAATTTTGGAAATGGATTACAGATGTTGGGTCAGAAACCAAAGACC
  AACATCGCCATCTTCTGTGAGACCAGGGCCGAGTGGATGATAGCTGCACAGGCGTGTTTT
  ATGTATAATTTTCAGCTTGTTACATTATATGCCACTCTAGGAGGTCCAGCCATTGTTCAT
  GCATTAAATGAAACAGAGGTGACCAACATCATTACTAGTAAAGAACTCTTACAAACAAAG
  TTGAAGGATATAGTTTCTTTGGTCCCACGCCTGCGGCACATCATCACTGTTGATGGAAAG
  CCACCGACCTGGTCCGAGTTCCCCAAGGGCATCATTGTGCATACCATGGCTGCAGTGGAG
  GCCCTGGGAGCCAAGGCCAGCATGGAAAACCAACCTCATAGCAAACCATTGCCCTCAGAT
  ATTGCAGTAATCATGTACACAAGTGGATCCACAGGACTTCCAAAGGGAGTCATGATCTCA
  CATAGTAACATTATTGCTGGTATAACTGGGATGGCAGAAAGGATTCCAGAACTAGGAGAG
  GAAGATGTCTACATTGGATATTTGCCTCTGGCCCATGTTCTAGAATTAAGTGCTGAGCTT
  GTCTGTCTTTCTCACGGATGCCGCATTGGTTACTCTTCACCACAGACTTTAGCAGATCAG
  TCTTCAAAAATTAAAAAAGGAAGCAAAGGGGATACATCCATGTTGAAACCAACACTGATG
  GCAGCAGTTCCGGAAATCATGGATCGGATCTACAAAAATGTCATGAATAAAGTCAGTGAA
  ATGAGTAGTTTTCAACGTAATCTGTTTATTCTGGCCTATAATTACAAAATGGAACAGATT
  TCAAAAGGACGTAATACTCCACTGTGCGACAGCTTTGTTTTCCGGAAAGTTCGAAGCTTG
  CTAGGGGGAAATATTCGTCTCCTGTTGTGTGGTGGCGCTCCACTTTCTGCAACCACGCAG
  CGATTCATGAACATCTGTTTCTGCTGTCCTGTTGGTCAGGGATACGGGCTCACTGAATCT
  GCTGGGGCTGGAACAATTTCCGAAGTGTGGGACTACAATACTGGCAGAGTGGGAGCACCA
  TTAGTTTGCTGTGAAATCAAATTAAAAAACTGGGAGGAAGGTGGATACTTTAATACTGAT
  AAGCCACACCCCAGGGGTGAAATTCTTATTGGGGGCCAAAGTGTGACAATGGGGTACTAC
  AAAAATGAAGCAAAAACAAAAGCTGATTTCTTTGAAGATGAAAATGGACAAAGGTGGCTC
  TGTACTGGGGATATTGGAGAGTTTGAACCCGATGGATGCTTAAAGATTATTGATCGTAAA
  AAGGACCTTGTAAAACTACAGGCAGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCT
  TTGAAGAATCTTCCACTAGTAGATAACATTTGTGCATATGCAAACAGTTATCATTCTTAT
  GTCATTGGATTTGTTGTGCCAAATCAAAAGGAACTAACTGAACTAGCTCGAAAGAAAGGA
  CTTAAAGGGACTTGGGAGGAGCTGTGTAACAGTTGTGAAATGGAAAATGAGGTACTTAAA
  GTGCTTTCCGAAGCTGCTATTTCAGCAAGTCTGGAAAAGTTTGAAATTCCAGTAAAAATT
  CGTTTGAGTCCTGAACCGTGGACCCCTGAAACTGGTCTGGTGACAGATGCCTTCAAGCTG
  AAACGCAAAGAGCTTAAAACACATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAA
  TAA
  
  Clone variation with respect to NM_203372.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_203372 unedited
  ACTTAGGGCGGCCGCGAATTCGGCACGAGGAACAGCTGTAACATTTGCCACCCTCAGAAG
  CTGCTGGTCCTGTGTCACACCACCTTAGCCTCTTGATCGAGGAAGATTCTCGCTGAAGTC
  TGTTAATTCTACTTTTTGAGTACTTATGAATAACCACGTGTCTTCAAAACCATCTACCAT
  GAAGCTAAAACATACCATCAACCCTATTCTTTTATATTTTATACATTTTCTAATATCACT
  TTATACTATTTTAACATACATTCCGTTTTATTTTTTCTCCGAGTCAAGACAAGAAAAATC
  AAACCGAATTAAAGCAAAGCCTGTAAATTCAAAACCTGATTCTGCATACAGATCTGTTAA
  TAGTTTGGATGGTTTGGCTTCAGTATTATACCCTGGATGTGATACTTTAGATAAAGTTTT
  TACATATGCAAAAAACAAATTTAAGAACAAAAGACTCTTGGGAACACGTGAAGTTTTAAA
  TGAGGAAGATGAAGTACAACCAAATGGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTA
  TAATTGGCTNTCCTATGAAGATGTCTTTGTTCGAGCCTTTAATTTGGAAAGGATACAGAT
  GNTGGGTCAGAACCAAAGACAACATCGNCATCTTCTGTGAACCAGGNCCGAGTGATGATA
  GCTGCACAGGCGTGTNTATGTATATTTTCAGCTTGTACATATATGCCATCTANGAGGTCA
  GNCATTGTCATGCATAATGAACAGAGTGACAACTCATACTATAAAGACCTACAACAAGTG
  AGGATTAGTTTTGGGCCCACGCTGCGAAAAATACTGTGTGGAAGCACGACTGGCCGGATC
  CAAGGCTATGGGCTACTGGTTGAGGGAGCTGGGCCAAGCCTTGAAAACAACTAAAAACCT
  GCCTAATTGGGATCTGCCAGGGGCCCGACTCAGGGGGGGGCCAAACCTTGGGGTACGGGG
  GAAGACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_203372 unedited
  GCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTAAAACATAAGTATGGGTAT
  ATTTATTTCTCTCAAATGCATACAAGACAATAATTACACAGCAACAAATCTTTTGTTCAA
  CAATGATTTGATTCATAAGCATTTGAAATTTACATAATTTCATATCAATACCCTTGTATT
  TTTAAATACAGTAAGTAAAAAAGCCCCCAAATAACCAATTCTTATATTTCCTATTTATCC
  CTCTATACATCCAAACTTTTAAAAAGTTACAAACTGAACATTATACAGAACATATAAATC
  ATGTTTAAAAACTTGAGGTTTTAAAATCACTGCTTCCCCAATATGATTCAGAAAAATTCT
  CATACTGACAAGTACAGTCATAGGTGGTAAAGTAAGTTGGTGGCGGGGAAAATGAAAGAA
  GAGACAGACACAAGTTTGCTGTCTTAATGTTTTACTAATCCTGTCAGTTAAAAATGGTGA
  CGTCATGAGAAGTAATAGTTTAATATGAGGAATGGAGTTTGCCTTGCAGCTTGAGACATG
  CATTTCAAGTATTTTCCTATTTGATCTGAGCTCACTGTAGCAAACTGATGCCAGAAGAGA
  ATAATTATTTTCTTCCATACATTCGCTCAATGTCCGCCTGGTAATGTGTTTTAAGCTCTT
  TGCGTTTCAGCTTGAAGGCATCTGTCACCAGACCAGTTTCAGGGGTCCACGTTTCAGGAC
  TCAAACGAATTTTTACTGGAATTTAAACTTTTCCAGACTTGCTGAAATAGCAGCTTCGGA
  AAGCACTTTAAGTACCTCATTTTNCATTTCACAACTGNTACACAGCTCCTCCCCAGTCCC
  TTTAAGTCCTTTCTTTCCAGCTAGNTCAAGTAGTTCCTTTGATTTTGCCCCACAAATCCA
  TGACATTAGAATGATACTGTTTGCCTATGCCAAATGTTATCTACTATGGAAGATTCTTAA
  AGCTGCTTTACTTTCCAGAAAAAATTTCNCCCTGCCGTAGTTTAAAGGGCCTTTTAGAAC
  AAATTTTTAAACTCATGGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_203372
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203372.1, NP_976251.1
• RefSeq Size: 4262 bp
• RefSeq ORF: 2163 bp
• Locus ID: 2181
• UniProt ID: O95573
• Cytogenetics: 2q36.1
• Protein Families: Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• Gene Summary: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.