POLR1D (NM_152705) Human Untagged Clone

CAT#: SC126842

POLR1D (untagged)-Human polymerase (RNA) I polypeptide D, 16kDa (POLR1D), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR1D
• Synonyms: AC19; POLR1C; RPA9; RPA16; RPAC2; RPC16; RPO1-3; TCS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126842 sequence for NM_152705 edited (data generated by NextGen Sequencing)
  ATGGAAGAGGATCAGGAGCTGGAGAGGAAAGCAATAGAAGAACTGCTTAAGGAGGCAAAA
  CGTGGGAAAACTAGAGCTGAAACAATGGGACCCATGGGTTGGATGAAGTGTCCTCTTGCT
  AGCACCAATAAAAGATTTCTAATTAACACAATTAAAAACACATTGCCCTCTCATAAAGAG
  CAAGACCATGAACAAAAAGAGGGCGATAAGGAACCAGCCAAGAGCCAGGCCCAGAAAGAA
  GAAAACCCAAAGAAACACAGAAGCCATCCTTACAAGCACAGCTTCCGCGCTCGAGGTTCC
  GCCAGTTACTCCCCGCCACGAAAGCGGAGCAGCCAGGACAAGTACGAAAAGCGGTCCAAC
  CGGCGGTGA
  
  Clone variation with respect to NM_152705.2
  219 g=>c;249 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152705 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCGCCTTCCGTC
  GGTCGGTCCTTGCTTCCTGCTTCGCCTCCGCGCCTCGCGCTATGGGACAGAGCCCCCGAT
  CCGCCAGCACCACCTGAGGATCCAGAAACCGCCCCAGCGATGGAAGAGGATCAGGAGCTG
  GAGAGGAAAGCAATAGAAGAACTGCTTAAGGAGGCAAAACGTGGGAAAACTAGAGCTGAA
  ACAATGGGACCCATGGGTTGGATGAAGTGTCCTCTTGCTAGCACCAATAAAAGATTTCTA
  ATTAACACAATTAAAAACACATTGCCCTCTCATAAAGAGCAAGACCATGAACAAAAAGAG
  GGCGATAAGGAACCAGCCAAGAGCCAGGCCCAGAAAGAAGAAAACCCAAAGAAACACAGA
  AGCCATCCTTACAAGCACAGCTTCCGCGCTCGAGGTTCCGCCAGTTACTCCCCGCCACGA
  AAGCGGAGCAGCCAGGACAAGTACGAAAAGCGGCCCAACCGGCGGTGAGGCTGGAACCAG
  TGCCAGCCAGCCCTGCGGGCCTCCGTGCTCCTTCGGGGTGCCGTGTGCGGAAAAAGCCTG
  AGCTGGCAGGGCAAACCCCTGAGAGTGACTTTGACATTACGTCCCTTTGTTGTTTTTAAA
  GAGGCTGCTGGCCACAGGCCAAAAAAAATGACCTAACACCTCTTGAAGAAACAAGTGCCA
  AGGAGGATGTCCTGGGCCAGTTTAACCTACTCCTGCCGGTAATACACTCACAATTGTTGG
  TTATCTGGTTGGCAGTGGTGTTAAAAATAACCAGTCAGAATTAAAGGTTTCACAAATGGT
  ACACCGGTTCCATTTTTGGTCTCTTCCAAAAAAAAAAACCTCAAACCCATTTTTACTTTT
  GGGGGTTCCTTTAACATACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152705
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152705.1, NP_689918.1
• RefSeq Size: 1977 bp
• RefSeq ORF: 369 bp
• Locus ID: 51082
• UniProt ID: P0DPB5
• Cytogenetics: 13q12.2
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS), a craniofacial development disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2011]
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct and shorter C-terminus, compared to isoform 1.