CD47 (NM_198793) Human Untagged Clone

CAT#: SC126757

CD47 (untagged)-Human CD47 molecule (CD47), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD47
• Synonyms: IAP; MER6; OA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126757 sequence for NM_198793 edited (data generated by NextGen Sequencing)
  ATGTGGCCCCTGGTAGCGGCGCTGTTGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTA
  CTATTTAATAAAACAAAATCTGTAGAATTCACGTTTTGTAATGACACTGTCGTCATTCCA
  TGCTTTGTTACTAATATGGAGGCACAAAACACTACTGAAGTATACGTAAAGTGGAAATTT
  AAAGGAAGAGATATTTACACCTTTGATGGAGCTCTAAACAAGTCCACTGTCCCCACTGAC
  TTTAGTAGTGCAAAAATTGAAGTCTCACAATTACTAAAAGGAGATGCCTCTTTGAAGATG
  GATAAGAGTGATGCTGTCTCACACACAGGAAACTACACTTGTGAAGTAACAGAATTAACC
  AGAGAAGGTGAAACGATCATCGAGCTAAAATATCGTGTTGTTTCATGGTTTTCTCCAAAT
  GAAAATATTCTTATTGTTATTTTCCCAATTTTTGCTATACTCCTGTTCTGGGGACAGTTT
  GGTATTAAAACACTTAAATATAGATCCGGTGGTATGGATGAGAAAACAATTGCTTTACTT
  GTTGCTGGACTAGTGATCACTGTCATTGTCATTGTTGGAGCCATTCTTTTCGTCCCAGGT
  GAATATTCATTAAAGAATGCTACTGGCCTTGGTTTAATTGTGACTTCTACAGGGATATTA
  ATATTACTTCACTACTATGTGTTTAGTACAGCGATTGGATTAACCTCCTTCGTCATTGCC
  ATATTGGTTATTCAGGTGATAGCCTATATCCTCGCTGTGGTTGGACTGAGTCTCTGTATT
  GCGGCGTGTATACCAATGCATGGCCCTCTTCTGATTTCAGGTTTGAGTATCTTAGCTCTA
  GCACAATTACTTGGACTAGTTTATATGAAATTTGTGGCTTCCAATCAGAAGACTATACAA
  CCTCCTAGGAATAACTGA
  
  Clone variation with respect to NM_198793.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198793 unedited
  TTTGTACTACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGCGGCCGTGCAG
  CCTGGGCAGTGGGTCCTGCCTGTGACGCGCGGCGGCGGTCGGTCCTGCCTGTAACGGCGG
  CGGCGGCTGCTGCTCCAGACACCTGCGGCGGCGGCGGCGACCCCGCGGCGGGCGCGGAGA
  TGTGGCCCCTGGTAGCGGCGCTGTTGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTAC
  TATTTAATAAAACAAAATCTGTAGAATTCACGTTTTGTAATGACACTGTCGTCATTCCAT
  GCTTTGTTACTAATATGGAGGCACAAAACACTACTGAAGTATACGTAAAGTGGAAATTTA
  AAGGAAGAGATATTTACACCTTTGATGGAGCTCTAAACAAGTCCACTGTCCCCACTGACT
  TTAGTAGTGCAAAAATTGAAGTCTCACAATTACTAAAAGGAGATGCCTCTTTGAAGATGG
  ATAAGAGTGATGCTGTCTCACACACAGGAAACTACACTTGTGAAGTAACAGAATTAACCA
  GAGAAGGTGAAACGATCATCGAGCTAAAATATCGTGTTGTTTCATGGTTTTCTCCAAATG
  AAAATATTCTTATTGTTATTTTCCCAATTTTTGCTATACTCCTGTTCTGGGGACAGTTTG
  GTATTAAAACACTTAAATATAGATCCGGTGGTATGGATGAGAAAACAATTGCTNTACTTG
  TTGCTGGACTAGTGATCACTGTCATTGTCATTGGTGGAGCCATTCTTTTCGTCCCCAGTG
  AATATTCATTAAAGAATGCTACTGGCNCTGGNNNTTATTGTGACTTCTCAGGGATATTAA
  TATTACTTCACTACTATGTNGTTNAGACAGCGATTGGATTAACCTNCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_198793 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAAAAAAGCTGTCTTGCTAGTA
  TGCTCAGTTTTCTGAGAGGCCTCAGCAGGTCATAAATTTAGGTTTGCCATGGGCAAACTA
  CTTGGTCCCAACATGAAATATGACAATCAATTTGGCATAAAAGAGGCACACGGGAAACAT
  CTGATGGACTAAGAAATAACTATTATTAATGCAACTACAAATATGAATATCTTATTACAC
  AAACAGGAAGAATTACGTATTTTTACAGGGTATTGGTGAGCAGTCAAAAAGCGTGGCAAA
  TTACCTAAAAGTTTTAAAAGTTTTAAGTGATCAAATATTTGCATCAAATATAATTCCACC
  CAATAAAGAACTTTGTATTTAAATGTTTTTACTAAAAGCACAAAATTAACCTTTGCTCTC
  CTGTAGGTACCCCACCTTTGTCCATACAGAAGATGCATGTGCCTATCTCATACGTATGCA
  CATACAAACACACATCCACAAACAGGTAAAAAACGAATGCTAAAAGTCTAAAAGTACTCC
  AGGCGATCACAACTGTCAGAACATTAATTTTCTTCTAACCAGGATAGGAAATGTGGGTTT
  TGTTTACTTTTCAATCATAGGGGAAGGGGAGTACGAGGCAGGACTACAGGAGGAAGGTGA
  TGAATGTTTATCACTGCACACAGATACAACAGTAATAAATCCCCATTCAAGGGCTGGCCT
  GTTGGCCTGATGGTGACACACATTTTGTTGCCATGCAGGAGACTCATAGTTCAAATTTCA
  ATCTTGCTTTTTTCATGCCCCAGATCAGAAAAGAGTGGAAGAACACTGTAGATTTTTGCC
  TTCAATCAGAGGAGGGGATACATATGTCACAACCTAGCTTTTTTGCTGAAAGTGTGAACC
  AGTGCTTGAGACAAGCTGGCTCCTCCTGCACGGGGCTACGGGGACTAAACATGTTAAAGA
  GGAAAAGTGCTCATCTTGACACAGAGCAAACCAGAGGCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198793
• Insert Size: 3210 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198793.2, NP_942088.1
• RefSeq Size: 5288 bp
• RefSeq ORF: 918 bp
• Locus ID: 961
• UniProt ID: Q08722
• Cytogenetics: 3q13.12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ECM-receptor interaction
• Gene Summary: This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (2) lacks two alternate exons compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.