PRPS1L1 (NM_175886) Human Untagged Clone

CAT#: SC126124

PRPS1L1 (untagged)-Human phosphoribosyl pyrophosphate synthetase 1-like 1 (PRPS1L1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRPS1L1
• Synonyms: PRPS1; PRPS3; PRPSL; PRS-III
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_175886 edited
  GGGGAGCTGGATCGCTCGACGGAGTGCCTCTGGTAGTTGGCCAAGACGCCGAATATCAAA
  ATCTTCAGCGGCAGCTCCCACCAGGACTTATCCCAGAAAATTGCTGACCGCCTGGGCCTG
  GAGCTAGGCAAGGTGGTGACTAAGAAATTCAGCAACCAGGAGACCTGCGTGGAAATTGAT
  GAGAGTGTGCGTGGAGAGGATGTCTACATCGTTCAGAGTGGTTGTGGCGAAATCAACGAC
  AGTCTAATGGAGCTTTTGATCATGATTAATGCCTGCAAGATTGCTTCAGCTAGCCGAGTT
  ACTGCAGTCATCCCATGCTTCCCTTATGCCCGACAGGATAAGAAGGATAAGAGCCGGTCC
  CCAATCTCTGCCAAGCTTGTTGCAAATATGCTCTCTATAGCAGGTGCGGATCATATCATC
  ACCATGGACCTACATGCTTCTCAAATTCAGGGCTTTTTTGATATCCCAGTAGACAACTTG
  TATGCAGAGCCAACTGTCCTGAAGTGGATAAGGGAGAATATCCCTGAGTGGAAGAACTGC
  ATTATTGTCTCGCCAGATGCTGGTGGAGCTAAAAGAGTGACCTCCATTGCAGACCAGTTG
  AATGTGGACTTTGCTTTGATTCATAAAGAACGGAAGAAGGCCAATGAAGTGGACTGCATA
  GTGCTAGTGGGAGATGTGAATGATCGTGTGGCTATCCTTGTAGATGACATGGCAGACACT
  TGTGTTACAATCTGCCTCGCAGCTGACAAACTTCTCTCAGCTGGAGCAACCAGAGTTTAT
  GCTATCTTGACTCATGGAATCTTTTCTGGCCCAGCCATTTCTCGCATCAACACTGCATGC
  TTTGAAGCAGTGGTAGTCACCAATACCATACCTCAAGATGATAAGATGAAGCATTGCTCC
  AAAATACGAGTAATTGACATCTCCATGATCCTTGCAGAAGCCATAAGGAGAACTCATAAT
  GGGGAATCTGTTTCCTACCTGTTCAGCCATGTTCCTTTATAACAGAATAACTTCTAGGTT
  ATGCTATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175886 unedited
  TCGGTTCATATTTGTATACGACTCATATAGGCGGCCGCGTAATTCGCCATTACGGCCGGG
  GAGCTGGATCGCTCGACGGATGCCTCTGGTAGTTGGCCAAGACGCCGAATATCAAAATCT
  TCAGCGGCAGCTCCCACCAGGACTTATCCCAGAAAATTGCTGACCGCCTGGGCCTGGAGC
  TAGGCAAGGTGGTGACTAAGAAATTCAGCAACCAGGAGACCTGCGTGGAAATTGATGAGA
  GTGTGCGTGGAGAGGATGTCTACATCGTTCAGAGTGGTTGTGGCGAAATCAACGACAGTC
  TAATGGAGCTTTTGATCATGATTAATGCCTGCAAGATTGCTTCAGCTAGCCGAGTTACTG
  CAGTCATCCCATGCTTCCCTTATGCCCGACAGGATAAGAAGGATAAGAGCCGGTCCCCAA
  TCTCTGCCAAGCTTGTTGCAAATATGCTCTCTATAGCAGGTGCGGATCATATCATCACCA
  TGGACCTACATGCTTCTCAAATTCAGGGCTTTTTTGATATCCCAGTAGACAACTTGTATG
  CAGAGCCAACTGTCCTGAAGTGGATAAGGGAGAATATCCCTGAGTGGAAGAACTGCATTA
  TTGTCTCGCCAGATGCTGGTGGAGCTAANAGAGTGACCTCCATTGCAGACCAGTTGAATG
  TGGACTTTGCTTTGATTCATAAAGAACGGAAGAAGGCCAATGAAGTGGACTGCATAGTGC
  TAGTGGGAGATGTGAATGATCGTGTGGCTATCCTTGTAGATGACATGGCAGACACTTGTG
  TTACAATCTGCCTCGCAGCTGAACAACTTCTCTCAGCTGGAGCAACCAGAGTTTATGCTA
  TCTTGACTCATGGAATCTTTTCTGGCCCACCATTTCTCGCATCACACTGCATGCTTTGAA
  GCAGTGGAANCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_175886
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175886.2, NP_787082.1
• RefSeq Size: 1091 bp
• RefSeq ORF: 957 bp
• Locus ID: 221823
• UniProt ID: P21108
• Cytogenetics: 7p21.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway, Purine metabolism
• Gene Summary: This intronless gene is specifically expressed in the testis, and encodes a protein that is highly homologous to the two subunits of phosphoribosylpyrophosphate synthetase encoded by human X-linked genes, PRPS1 and PRPS2. These enzymes convert pyrimidine, purine or pyridine bases to the corresponding ribonucleoside monophosphates. In vitro transcription/translation and site-directed mutagenesis studies indicate that translation of this mRNA initiates exclusively at a non-AUG (ACG) codon. [provided by RefSeq, Jul 2008]