BRMS1 (BC009834) Human Untagged Clone

CAT#: SC125940

BRMS1 (untagged)-Human breast cancer metastasis suppressor 1, mRNA (cDNA clone MGC:15101 IMAGE:3959486), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRMS1
• Synonyms: breast cancer metastasis-suppressor 1; breast cancer metastasis suppressor 1; DKFZp564A063
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC009834 edited
  GCGCCTACGGGAGTCCGGCGGCAGCAGCTGGTACCGGCAACCACGGGCAGCTCTCAGGGA
  ATCTCCGTCGTGAGGCCAGAGGCTCCAGTCCCCGCGAGTCCAGATGCCTGTCCAGCCTCC
  AAGCAAAGACACAGAAGAGATGGAAGCAGAGGGTGATTCTGCTGCTGAGATGAATGGGGA
  GGAGGAAGAGAGTGAGGAGGAGCGGAGCGGCAGCCAGACAGAGTCAGAAGAGGAGAGCTC
  CGAGATGGATGATGAGGACTATGAGCGACGCCGCAGCGAGTGTGTCAGTGAGATGCTGGA
  CCTAGAGAAGCAGTTCTCGGAGCTAAAGGAGAAGTTGTTCAGGGAACGACTGAGTCAGCT
  GCGGTTGCGGCTGGAGGAAGTGGGGGCTGAGAGAGCCCCTGAATACACGGAGCCCCTTGG
  GGGGCTGCAGCGGAGCCTCAAGATTCGCATTCAGGTGGCAGGGATCTACAAGGGCTTCTG
  TCTGGATGTGATCAGGAATAAGTACGAATGTGAGCTGCAGGGAGCCAAACAGCACCTGGA
  GAGTGAGAAGCTGCTGCTCTATGACACGCTGCAGGGGGAGCTGCAGGAGCGGATCCAGAG
  GCTGGAGGAGGACCGCCAGAGCCTGGATCTCAGCTCTGAATGGTGGGACGACAAACTGCA
  CGCCAGAGGCAGCTCCAGGTCTTGGGACTCCCTGCCGCCCAGCAAGAGGAAGAAGGCACC
  TCTGGTTTCTGGCCCATACATCGTGTACATGCTTCAAGAGATCGACATCCTGGAGGACTG
  GACAGCCATCAAAAAGGCTAGGGCAGCTGTGTCCCCTCAGAAGAGAAAATCGGATGGACC
  TTGACCCTGCTGTTCACAGCCAGGGGGACCCTCAGAGCAGCTGGCACTGCACCCAGGATT
  CTCGTCTTCCTCCTGCAGACAGGCGGACCCACAGGCCCCTCAGGGTCTGCCCAGCCAGGC
  TCCTGTGGTGCTGCTGGGCCCTCCCACTCCATCTGGCACTGGCCTGGACTCCTCCTCTGC
  CCTCCTCGAGGCCTGCACAGCTGTGGCCGTGGAGCTGACCTGACCAGGCAAGGCTGCTGT
  CTCCATCCCTGAGCCGCCTGCCACCTCCCACTCCTGAAGATCCATCTCTTGGGGCTCCCC
  TGACAGAGAAGACAGCCGAAGTCAAAGCCACATCCTCTTGCTGATGTTGGATGCAGGCTG
  TCCGGCCTCAGGGCCAGGGAGCCAGTTTCCACTGTGCGGGAACTCTGAGTCAGACGTGAT
  TATCTGGGGGTCTGTCCACCCTGGCTGGATCTGGAGGCAAGATGCCAGGCCCCCCAGGTG
  TTCTCAGGGCAGTTCTTGGTGTCTGCTTCTCAGATTCCAAGGACTGGAATTAAAACCTTT
  CCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC009834 unedited
  NCCCGTTCAGAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGC
  CTACGGGAGTCCGGCGGCAGCAGCTGGTACCGGCAACCACGGGCAGCTCTCAGGGAATCT
  CCGTCGTGAGGCCAGAGGCTCCAGTCCCCGCGAGTCCAGATGCCTGTCCAGCCTCCAAGC
  AAAGACACAGAAGAGATGGAAGCAGAGGGTGATTCTGCTGCTGAGATGAATGGGGAGGAG
  GAAGAGAGTGAGGAGGAGCGGAGCGGCAGCCAGACAGAGTCAGAAGAGGAGAGCTCCGAG
  ATGGATGATGAGGACTATGAGCGACGCCGCAGCGAGTGTGTCAGTGAGATGCTGGACCTA
  GAGAAGCAGTTCTCGGAGCTAAAGGAGAAGTTGTTCAGGGAACGACTGAGTCAGCTGCGG
  TTGCGGCTGGAGGAAGTGGGGGCTGAGAGAGCCCCTGAATACACGGAGCCCCTTGGGGGG
  CTGCAGCGGAGCCTCAAGATTCGCATTCAGGTGGCAGGGATCTACAAGGGCTTCTGTCTG
  GATGTGATCAGGAATAAGTACGAATGTGAGCTGCAGGGAGCCAAACAGCACCTGGAGAGT
  GAGAAGCTGCTGCTCTATGACACGCTGCAGGGGGAGCTGCAGGAGCGGATCCAGAGGCTG
  GAGGAGGACCGCCAGAGCCTGGATCTCAGCTCTGAATGGTGGGACGACAAACTGCACGCC
  AGAAGCAGCTCCAGGTCTTGGGACTCCCTGCCGCCCAGCAAGAGGAAGAAAGCACCTCTG
  GTTTCTGGGCCATACATCGTGTACATGCTTCAAGAGATCGACATCCCTGGAAGACTGGAC
  AGCCATCAAAAGGCTAGGGCAGCTGTGTCCCCTCAAAAGAAAAAATCGATGGACCTTGAC
  CCTGCTGT
  
• Restriction Sites: Please inquire
• ACCN: BC009834
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009834.2, AAH09834.1
• RefSeq Size: 1438 bp
• Locus ID: 25855
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Gene Summary: This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]