MEF2D (BC040949) Human Untagged Clone

CAT#: SC125772

MEF2D (untagged)-Human MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancer factor 2D) (cDNA clone MGC:48824 IMAGE:6044434), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEF2D
• Synonyms: DKFZp686I1536; MADS box transcription enhancer factor 2, polypeptide D (myocyte enhancer factor 2D); MEF2D/DAZAP1 fusion; myocyte enhancer factor 2D; myocyte enhancer factor 2D/deleted in azoospermia associated protein 1 fusion protein; OTTHUMP00000031810
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC040949 edited
  GGGGCCTGAGACCACCCCTGCCCTAGGCCCAGCTTTCCTGGACTGCCTGCCCCCACAAAC
  CAACAGCCCGCCCCCAGGTCCCCTGTCGAAGGTATCCTGCAGACCATGAACTGAGCACTG
  TTCCCAGACCGTTCATGAGCACAGTGTAAGGTGTGCCGAGACCCACCACCCAGCGAGCCC
  CTCCCCTCCGTAGCACTGAGGACCCCCGGAGAAGATGGGGAGGAAAAAGATTCAGATCCA
  GCGAATCACCGACGAGCGGAACCGACAGGTGACTTTCACCAAGCGGAAGTTTGGCCTGAT
  GAAGAAGGCGTATGAGCTGAGCGTGCTATGTGACTGCGAGATCGCACTCATCATCTTCAA
  CCACTCCAACAAGCTGTTCCAGTACGCCAGCACCGACATGGACAAGGTGCTGCTCAAGTA
  CACGGAGTACAATGAGCCACACGAGAGCCGCACCAACGCCGACATCATCGAGACCCTGAG
  GAAGAAGGGCTTCAATGGCTGCGACAGCCCCGAGCCCGACGGGGAGGACTCGCTGGAACA
  GAGCCCCCTGCTGGAGGACAAGTACCGACGCGCCAGCGAGGAGCTCGACGGGCTCTTCCG
  GCGCTATGGGTCAACTGTCCCGGCCCCCAACTTTGCCATGCCTGTCACGGTGCCCGTGTC
  CAATCAGAGCTCACTGCAGTTCAGCAATCCCAGCGGCTCCCTGGTCACCCCTTCCCTGGT
  GACATCATCCCTCACGGACCCGCGGCTCCTGTCCCCCCAGCAGCCAGCACTACAGAGGAA
  CAGTGTGTCTCCTGGCCTGCCCCAGCGGCCAGCTAGTGCGGGGGCCATGCTGGGGGGTGA
  CCTGAACAGTGCTAACGGAGCCTGCCCCAGCCCTGTTGGGAATGGCTACGTCAGTGCTCG
  GGCTTCCCCTGGCCTCCTCCCTGTGGCCAATGGCAACAGCCTAAACAAGGTCATCCCTGC
  CAAGTCTCCGCCCCCACCTACCCACAGCACCCAGCTTGGAGCCCCCAGCCGCAAGCCCGA
  CCTGCGAGTCATCACTTCCCAGGCAGGAAAGGGGTTAATGCATCACTTGAACAATGCCCA
  GCGCCTTGGGGTCTCCCAGTCTACTCATTCGCTCACCACCCCAGTGGTTTCTGTGGCAAC
  GCCGAGTTTACTCAGCCAGGGCCTCCCCTTCTCTTCCATGCCCACTGCCTACAACACAGA
  TTACCAGTTGACCAGTGCAGAGCTCTCCTCCTTACCAGCCTTTAGTTCACCTGGGGGGCT
  GTCGCTAGGCAATGTCACTGCCTGGCAACAGCCACAGCAGCCCCAGCAGCCGCAGCAGCC
  ACAGCCTCCACAGCAGCAGCCACCGCAGCCACAGCAGCCACAGCCACAGCAGCCTCAGCA
  GCCGCAACAGCCACCTCAGCAACAGTCCCACCTGGTCCCTGTATCTCTCAGCAACCTCAT
  CCCGGGCAGCCCCCTGCCCCACGTGGGTGCTGCCCTCACAGTCACCACCCACCCCCACAT
  CAGCATCAAGTCAGAACCGGTGTCCCCAAGCCGTGAGCGCAGCCCTGCGCCTCCCCCTCC
  AGCTGTGTTCCCAGCTGCCCGCCCTGAGCCTGGCGATGGTCTCAGCAGCCCAGCCGGGGG
  ATCCTATGAGACGGGAGACCGGGATGACGGACGGGGGGACTTCGGGCCCACACTGGGCCT
  GCTGCGCCCAGCCCCAGAGCCTGAGGCTGAGGGCTCAGCTGTGAAGAGGATGCGGCTTGA
  TACCTGGACATTAAAGTGACGATTCCCACTCCCCTCCTCTCAGCCTCCCTGATGAAGAGT
  TGACAATCTCACCGCCCGCCCTTCCCTGCCCCGGGCTCCTCCCGCTCGACCCCCACTTCC
  TTTCTTGTGCTTCGTGTCCTGTTGACGGTTACATTTGTGTATAATTATTATATTATTATT
  ATTATTATTATATTTTTTTTAATTTGGATTCTCGCTTTGGAGAGGGGGATGCTCTCATCC
  CCTCTTTCTGTACCCCCCACCATTTTCACTGGCTGGGGGGCTCTCTTTTTCGCGGGAAGG
  GGGGACACTTTGCACGTTGTACACATATGCTGCAGGAAGGGGGTGGGGGGCCCAATAAGG
  CCTTTGGGAAAGGACAGGTGCCGAGCCCTGCATGTGGAGCCCTCCCACCCCACCCCCAGA
  TAGAGGGAAATAACCAAAAAACTACCAAACAACAGAAACCCACACTCTAGACTGAAACCC
  CAAAGTGGGCTTGATGGGTGGGTTTGTGTTTCAAGGGGAAAGTGAGGCAGAGGTTCTGAA
  AAGGGTCTCTGTTTTTGTGTTCATGTAGCCATAGGCACATGGAGCAGAATACTTAAGCCT
  GGCCCCCAAATGCCCCTGCACACACACGTGCCACACCTGCGCTGATTCTTGTGTGTGCTG
  CACCCCCAAGGTGTGTGGGTGCTGGCTGAGCTTTGGGCCGGGAAGGCAGCCTGGGAATCT
  GAGGCTGGAGACAGGGGTTTGAGGTGGGGGCCTCTCTGGAAGCACATTTGGAGGGAAAGA
  CAAGAGAGCCATGAGGAGAGGGCTGAGGAGGGCAGAAGGGCTAGGCAGGGGGCAAATTGA
  GCCCCTCCCTTCCCCAGTTTTTCTCTAAGATATACAGTGCAATAGCTCCCCACCCCTCAG
  TTGACGCCAGCCCTGTAAAGCTGGCCACAGTGTGCAGGGAGAATGGGGAGAGGGTCTTCA
  GTGAGGTGGCTGGGGCGAGAGTCGGCCTGGACTTCCCTGGGGTGCTCCAGGCCAGAGCTC
  TTTCATTGGGGCGAGTGTGGTGAGGGGACGTCCTTGGTCTTGCACGCACACTACCTGGGG
  GAGTCAACACTGGGATGGTCTGTGGGGTGGGAGGGCCTACGGATGGGTCCGTAGAGGTCC
  CACCTCCCTCATTCCTCCTTGGCCCCTCTCCCTAGCTTCTCCTGTTAGCTCCTTCTGCTC
  CTGACCCCACCTCCTTGCTCTTGGCGCCCCTATTGTCTCTGGCTACCTCCTTGTCCCACC
  ACCTCCAGGCTGCATCCCACCTTCCCTCTTGGCTACTGTAATTGTAAATAGCGACCTTTG
  GAAAACGTTAGCGGTGTAACAGTCCAGGAAACTGTTTTTTTTTGTTGTTGTTGTATTGAT
  ATGAAATGAGATTCTATTTTTGTCAAAGTATATTGTAATAATAATGACTCAAACGGCCCG
  TACTGTACAGACGAAAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: BC040949
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC040949.1, AAH40949.1
• RefSeq Size: 3210 bp
• Locus ID: 4209
• Cytogenetics: 1q22
• Protein Families: Transcription Factors
• Gene Summary: This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]