SUV39H2 (NM_024670) Human Untagged Clone

CAT#: SC125719

SUV39H2 (untagged)-Human suppressor of variegation 3-9 homolog 2 (Drosophila) (SUV39H2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SUV39H2
• Synonyms: KMT1B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024670 edited
  CGGGGCCGAGGCGCGAGGAGGTGAGGCTGGAGCGCGGCCCCCTCGCCTTCCCTGTTCCCA
  GGCAAGCTCCCAAGGCCCGGGCGGCGGGGCCGTCCCGCGGGCCAGCCAGATGGCGACGTG
  GCGGTTCCCCGCCCGCCGCGACCCCAACTCCGGGACGCACGCTGCGGACGCCTATCCTCC
  CCCAGGCCGCTGACCCGCCTCCCTGCCCGGCCGGCTCCCGCCGCGGAGGATATGGAATAT
  TATCTTGTAAAATGGAAAGGATGGCCAGATTCTACAAATACTTGGGAACCTTTGCAAAAT
  CTGAAGTGCCCGTTACTGCTTCAGCAATTCTCTAATGACAAGCATAATTATTTATCTCAG
  GTAAAGAAAGGCAAAGCAATAACTCCAAAAGACAATAACAAAACTTTGAAACCTGCCATT
  GCTGAGTACATTGTGAAGAAGGCTAAACAAAGGATAGCTCTGCAGAGATGGCAAGATGAA
  CTCAACAGAAGAAAGAATCATAAAGGAATGATATTTGTTGAAAATACTGTTGATTTAGAG
  GGCCCACCTTCAGACTTCTATTACATTAACGAATACAAACCAGCTCCTGGAATCAGCTTA
  GTCAATGAAGCTACCTTTGGTTGTTCATGCACAGATTGCTTCTTTCAAAAATGTTGTCCT
  GCTGAAGCTGGAGTTCTTTTGGCTTATAATAAAAACCAACAAATTAAAATCCCACCTGGT
  ACTCCCATCTATGAATGCAACTCAAGGTGTCAGTGTGGTCCTGATTGTCCCAATAGGATT
  GTACAAAAAGGCACACAGTATTCGCTTTGCATCTTTCGAACTAGCAATGGACGTGGCTGG
  GGTGTAAAGACCCTTGTGAAGATTAAAAGAATGAGTTTTGTCATGGAATATGTTGGAGAG
  GTAATCACAAGTGAAGAAGCTGAAAGACGAGGACAGTTCTATGACAACAAGGGAATCACG
  TATCTCTTTGATCTGGACTATGAGTCTGATGAATTCACAGTGGATGCGGCTCGATACGGC
  AATGTGTCTCATTTTGTGAATCACAGCTGTGACCCAAATCTTCAGGTGTTCAATGTTTTC
  ATTGATAACCTCGATACTCGTCTTCCCCGAATAGCATTGTTTTCCACAAGAACCATAAAT
  GCTGGAGAAGAGCTGACTTTTGATTATCAAATGAAAGGTTCTGGAGATATATCTTCAGAT
  TCTATTGACCACAGCCCAGCCAAAAAGAGGGTCAGAACAGTATGTAAATGTGGAGCTGTG
  ACTTGCAGAGGTTACCTCAACTGAACTTTTTCAGGAAATAGAGCTGATGATTATAATATT
  TTTTTCCTAATGTTAACATTTTTAAAAATACATATTTGGGACTCTTATTATCAAGGTTCT
  ACCTATGTTAATTTACAATTCATGTTTCAAGACATTTGCCAAATGTATTACCGATGCCTC
  TGAAAAGGGGGTCACTGGGTCTCATAGACTGATATGAAGTCGACATATTTATAGTGCTTA
  GAGACCAAACTAATGGAAGGCAGACTATTTACAGCTTAGTATATGTGTACTTAAGTCTAT
  GTGAACAGAGAAATGCCTCCCGTAGTGTTTGAAAGCGTTAAGCTGATAATGTAATTAACA
  ACTGCTGAGAGATCAAAGATTCAACTTGCCATACACCTCAAATTCGGAGAAACAGTTAAT
  TTGGGCAAATCTACAGTTCTGTTTTTGCTACTCTATTGTCATTCCTGTTTAATACTCACT
  GTACTTGTATTTGAGACAAATAGGTGATACTGAATTTTATACTGTTTTCTACTTTTCCAT
  TAAAACATTGGCACCTCAATGATAAAGAAATTTAAGGTATAAAATTAAATGTAAAAATTA
  ATTTCAGCTTCATTTCGTATTTCGAAGCAATCTAGACTGTTGTGATGAGTGTATGTCTGA
  ACCTGTAATTCTTAAAAGACTTCTTAATCTTCTAGAAGAAAAATCTCCGAAGAGCTCTCT
  CTAGAAGTCCAAAATGGCTAGCCATTATGCTTCTTTGAAAGGACATGATAATGGGACCAG
  GATGGTTTTTTGGAGTACCAAGCAAGGGGAATGGAGCACTTTAAGGGCGCCTGTTAGTAA
  CATGAATTGGAAATCTGTGTCGAGTACCTCTGATCTAAACGGTAAAACAAGCTGCCTGGA
  GAGCAGCTGTACCTAACAATACTGTAATGTACATTAACATTACAGCCTCTCAATTTCAGG
  CAGGTGTAACAGTTCCTTTCCACCAGATTTAATATTTTTATACTTCCTGCAGGTTCTTCT
  TAAAAAGTAATCTATATTTTTGAACTGATACTTGTTTTATACATAAATTTTTTTTAGATG
  TGATAAAGCTAAACTTGGCCAAAGTGTGTGCCTGAATTATTAGACCTTTTTATTAGTCAA
  CCTACGAAGACTAAAATAGAATATATTAGTTTTCAAGGGAGTGGGAGGCTTCCAACATAG
  TATTGAATCTCAGGAAAAACTATTCTTTCATGTCTGATTCTGAGATTTCTAATTGTGTTG
  TGAAAATGATAAATGCAGCAAATCTAGCTTTCAGTATTCCTAATTTTTACCTAAGCTCAT
  TGCTCCAGGCTTTGATTACCTAAAATAAGCTTGGATAAAATTGAACCAACTTCAAGAATG
  CAGCACTTCTTAATCTTTAGCTCTTTCTTGGGAGAAGCTAGACTTTATTCATTATATTGC
  TATGACAACTTCACTCTTTCATAATATATAGGATAAATTGTTTACATGATTGGACCCTCA
  GATTCTGTTAACCAAAATTGCAGAATGGGGGGCCAGGCCTGTGTGGTGGCTCACACCTGT
  GATCCCAGCACTTTGGGAGGCTGAGGTAGGAGGATCACGTGAGGTCGGGAGTTCAAGACC
  AGCCTGGCCATCATGGTGAAACCCTGTCTCTACTGAAAATACAAAAATTAGCCGGGCGTG
  GTGGCACACGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAATT
  CAGGAGGCGGAGGTTGCAGTGAGCCAAGATCATACCACTGCACTGCAGCCTGAGTGACAC
  AGTAAGACTGTCTCCAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024670 unedited
  NNCCGTTCAGATTTTGTAATACGACTCACTATAGGGNCGGACCGCGNAATTCGGCACGAG
  GCGGGGCCGAGGCGCGAGGAGGTGAGGCTGGAGCGCGGCCCCCTCGCCTTCCCTGTTCCC
  AGGCAAGCTCCCAAGGCCCGGGCGGCGGGGCCGTCCCGCGGGCCAGCCAGATGGCGACGT
  GGCGGTTCCCCGCCCGCCGCGACCCCAACTCCGGGACGCACGCTGCGGACGCCTATCCTC
  CCCCAGGCCGCTGACCCGCCTCCCTGCCCGGCCGGCTCCCGCCGCGGAGGATATGGAATA
  TTATCTTGTAAAATGGAAAGGATGGCCAGATTCTACAAATACTTGGGAACCTTTGCAAAA
  TCTGAAGTGCCCGTTACTGCTTCAGCAATTCTCTAATGACAAGCATAATTATTTATCTCA
  GGTAAAGAAAGGCAAAGCAATAACTCCAAAAGACAATAACAAAACTTTGAAACCTGCCAT
  TGCTGAGTACATTGTGAAGAAGGCTAAACAAAGGATAGCTCTGCAGAGATGGCAAGATGA
  ACTCAACAGAAGAAAGAATCATAAAGGAATGATATTTGTTGAAAATACTGTTGATTTAGA
  GGGCCCACCTTCAGACTTCTATTACATTAACGAATACAAACCAGCTCCTGGAATCAGCTT
  AGTCAATGAAGCTACCTTTGGTTGTTCATGCACAGATTGCTTCTTTCAAAAATGTTGTCC
  TGCTGAAGCTGGAGTTCTTTTGGCTTATAATAAAAACCAANCAATTAAAATCCCACCTGG
  TACTCCCATCTATGAATGCAACTCAAGGTGTCAGTGTGGTCCTGATTGTCCCATAGGATT
  GTACAAAAGGCACACAGTATTCGCTTTGCATCTTTCGAACTAGCAATGGACGTGGCTGGG
  GTGTAAAGACCCCG
  
• Restriction Sites: Please inquire
• ACCN: NM_024670
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024670.3, NP_078946.1
• RefSeq Size: 3093 bp
• RefSeq ORF: 1053 bp
• Locus ID: 79723
• UniProt ID: Q9H5I1
• Cytogenetics: 10p13
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• Gene Summary: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains an alternate 5' terminal exon, and is missing the subsequent exon compared to variant 1. This results in translation initiation from an in-frame, downstream AUG, and a shorter isoform (2) compared to isoform 1. Variants 2 and 3 encode the same isoform.