PSMA8 (NM_001025097) Human Untagged Clone

CAT#: SC125657

PSMA8 (untagged)-Human proteasome (prosome, macropain) subunit, alpha type, 8 (PSMA8), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMA8
• Synonyms: PSMA7L
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001025097 edited
  AGCGGGCTTTCGGGCGGTAGCACGCTGTGTTGGCGGCGGCTCCCCGCTTGCCTCAGCTGC
  AGCAGCGGGAAGCTCGGTGGCAAGCCCTTGTAGTCCTGTGCGATGGCGTCTCGATATGAC
  AGGGCGATCACTGTCTTCTCCCCAGACGGACACCTTTTTCAAGTTGAATATGCCCAGGAA
  GCGGTGAAGAAAGGATCCACCGCGGTCGGAATTCGAGGACTTACTGCTGATGCTAGAGTA
  GTAATAAACAGAGCCCGTGTGGAGTGCCAGAGCCATAAGCTTACGGTTGAGGACCCAGTC
  ACTGTAGAATACATAACTCGCTTCATAGCAACTTTAAAGCAGAAATATACCCAAAGCAAT
  GGACGAAGACCTTTTGGTATTTCTGCCTTAATTGTAGGTTTTGATGATGATGGTATCTCA
  AGATTGTATCAGACAGATCCTTCTGGTACTTATCATGCTTGGAAGGCAAATGCAATAGGC
  CGAAGTGCTAAAACTGTTCGAGAATTTCTAGAAAAGAATTACACAGAAGATGCCATAGCA
  AGTGACAGTGAAGCTATCAAGTTAGCAATAAAAGCTTTGCTAGAAGTTGTCCAGTCTGGT
  GGAAGAAACATTGAACTTGCTATAATAAGAAGAAATCAACCTTTGAAGATGTTTAGTGCA
  AAAGAAGTTGAATTATATGTAACTGAAATAGAAAAGGAAAAGGAAGAAGCAGAGAAGAAA
  AAATCAAAGAAATCTGTCTAATTCTTAGGATGACCACTGGGAGGTCTTAATGTTTTGTTT
  TATTGTACTGCCTGAGGTTGTTTAGTGAAATTTTAGAGGAAAACAGTTATTTTGCAGCAT
  TACATGCAGTACTTGTGTGATGTTTTGAGAATGCCAGATCTGTGGCTGTCTTCATTCTAT
  TACATAGTCAAACATAGGTTTATGTGAAGATTTTCTTTGAAAGGGGATTTCAGTAATTGT
  TGAGAGCAGTCATAATTCCACATAAGCCTGAGACTCTATAATTTGTCCAGTGTCTTACTT
  ACCTTCATATATGCAAATATAATTTTAAAAATTTTTTAATTAAAAAAATTTAATAATTTG
  GTGATGTTTGAATATTGACTGTCAGTTAAGGACATAGCTATTCCAAATAAACTAAATATA
  ATATTGAGCTGCATTTCTCAAAGAATGACAAAAGCATCCTTATACTGATGAGTTCTTAAA
  ATTTTGTCAGAGCCTTCTTCATTTTACAATATTTTGTTATTTTTATTTTACCTTTTTCAT
  TTTACAATATTTCGTTATTTTTATTTTACCTTTTTCATTTTACAAGTTGCAATATATTGT
  TTCGTTCATGGAACAAATATAGGCTGAATTCAAACATGCAATTAAAGCATGGGAAGAGCT
  TCTAAGGGCATCTTAACAGGATGCTACATGAAAATAAGGAAAGAAATTAATACATATCAC
  TTTTAGTTTAGATTTTTATTTTATCTCAAGTATGTCCAGTGTGTTCGGTTTGTTACATAT
  TCCATGAAATATCTGAGTGTGTATTTTATGATTGGTATTATGGAAGGCTTATTGGGGGAA
  CAAGAAAAATATAAAAAGTGATCTCTGCCCACGGAAAGGATTTAATCCAAATGGTCCACA
  AGAGGAATGTGCTTAATTCCTACAGTGAGTTTATATTTTTGAAAATAAAAGCTAGTAAAT
  ATTCAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001025097 unedited
  NGGGGGTCAGATTTTGTATACGACTCATATAGGCGGCCGCATAACTTCGTATAGCATACA
  TTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGCTT
  TCGGGCGGTAGCACGCTGTGTTGGCGGCGGCTCCCCGCTTGCCTCAGCTGCAGCAGCGGG
  AAGCTCGGTGGCAAGCCCTTGTAGTCCTGTGCGATGGCGTCTCGATATGACAGGGCGATC
  ACTGTCTTCTCCCCAGACGGACACCTTTTTCAAGTTGAATATGCCCAGGAAGCGGTGAAG
  AAAGGATCCACCGCGGTCGGAATTCGAGGACTTACTGCTGATGCTAGAGTAGTAATAAAC
  AGAGCCCGTGTGGAGTGCCAGAGCCATAAGCTTACGGTTGAGGACCCAGTCACTGTAGAA
  TACATAACTCGCTTCATAGCAACTTTAAAGCAGAAATATACCCAAAGCAATGGACGAAGA
  CCTTTTGGTATTTCTGCCTTAATTGTAGGTTTTGATGATGATGGTATCTCAAGATTGTAT
  CAGACAGATCCTTCTGGTACTTATCATGCTTGGAAGGCAAATGCAATAGGCCGAAGTGCT
  AAAACTGTTCGAGAATTTCTAGAAAAGAATTACACAGAAGATGCCATAGCAAGTGACAGT
  GAAGCTATCAAGTTAGCAATAAAAGCTTTGCTAGAAGTTGTCCAGTCTGGTGGAAGAAAC
  ATTGAACTTGCTATAATAAGAAGAAATCAACCTTTGAAGATGTTTAGTGCAAAAGAAGTT
  GAATTATATGTAACTGANATAGAAAAGGAAAAGGAAGAAGCAGAGAAGAANAAATCAAAA
  AAATCTGTCTAATTCTTAGGATGACCACTGNGAGGTCTTAATGGGTTTGTTTATTGTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001025097
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025097.1, NP_001020268.1
• RefSeq Size: 1713 bp
• RefSeq ORF: 639 bp
• Locus ID: 143471
• UniProt ID: Q8TAA3
• Cytogenetics: 18q11.2
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: Component of the spermatoproteasome, a form of the proteasome specifically found in testis that promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses two different alternate in-frame splice sites in the coding region, compared to variant 1, resulting in a shorter isoform (3).