SIRP alpha (SIRPA) (BC026692) Human Untagged Clone

CAT#: SC125649

SIRPA (untagged)-Human protein tyrosine phosphatase, non-receptor type substrate 1, mRNA (cDNA clone MGC:9637 IMAGE:3916836), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIRP alpha
• Synonyms: BIT; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD172A; macrophage fusion receptor; MFR; MYD-1; myd-1 antigen; OTTHUMP00000030001; P84; prote; PTPNS1; SHPS-1; SHPS1; SHP substrate-1; SIRP; SIRP-ALPHA-1; SIRPalpha; SIRPalpha2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC026692 edited
  CGCCGTGTGGAGCCCGGGCGGGGCAGCCGCGGCCCATGGAGCCCGCCGGCCCGGCCCCCG
  GCCGCCTCGGGCCGCTGCTCTGCCTGCTGCTCGCCGCGTCCTGCGCCTGGTCAGGAGTGG
  CGGGTGAGGAGGAGCTGCAGGTGATTCAGCCTGACAAGTCCGTATCAGTTGCAGCTGGAG
  AGTCGGCCATTCTGCACTGCACTGTGACCTCCCTGATCCCTGTGGGGCCCATCCAGTGGT
  TCAGAGGAGCTGGACCAGCCCGGGAATTAATCTACAATCAAAAAGAAGGCCACTTCCCCC
  GGGTAACAACTGTTTCAGAGTCCACAAAGAGAGAAAACATGGACTTTTCCATCAGCATCA
  GTAACATCACCCCAGCAGATGCCGGCACCTACTACTGTGTGAAGTTCCGGAAAGGGAGCC
  CTGACACGGAGTTTAAGTCTGGAGCAGGCACTGAGCTGTCTGTGCGTGCCAAACCCTCTG
  CCCCCGTGGTATCGGGCCCTGCGGCGAGGGCCACACCTCAGCACACAGTGAGCTTCACCT
  GCGAGTCCCACGGCTTCTCACCCAGAGACATCACCCTGAAATGGTTCAAAAATGGGAATG
  AGCTCTCAGACTTCCAGACCAACGTGGACCCCGTAGGAGAGAGCGTGTCCTACAGCATCC
  ACAGCACAGCCAAGGTGGTGCTGACCCGCGAGGACGTTCACTCTCAAGTCATCTGCGAGG
  TGGCCCACGTCACCTTGCAGGGGGACCCTCTTCGTGGGACTGCCAACTTGTCTGAGACCA
  TCCGAGTTCCACCCACCTTGGAGGTTACTCAACAGCCCGTGAGGGCAGAGAACCAGGTGA
  ATGTCACCTGCCAGGTGAGGAAGTTCTACCCCCAGAGACTACAGCTGACCTGGTTGGAGA
  ATGGAAACGTGTCCCGGACAGAAACGGCCTCAACCGTTACAGAGAACAAGGATGGTACCT
  ACAACTGGATGAGCTGGCTCCTGGTGAATGTATCTGCCCACAGGGATGATGTGAAGCTCA
  CCTGCCAGGTGGAGCATGACGGGCAGCCAGCGGTCAGCAAAAGCCATGACCTGAAGGTCT
  CAGCCCACCCGAAGGAGCAGGGCTCAAATACCGCCGCTGAGAACACTGGATCTAATGAAC
  GGAACATCTATATTGTGGTGGGTGTGGTGTGCACCTTGCTGGTGGCCCTACTGATGGCGG
  CCCTCTACCTCGTCCGAATCAGACAGAAGAAAGCCCAGGGCTCCACTTCTTCTACAAGGT
  TGCATGAGCCCGAGAAGAATGCCAGAGAAATAACACAGGTACAGTCCTTGGACACAAATG
  ATATCACATATGCAGACCTGAACCTGCCCAAGGGGAAGAAGCCTGCTCCCCAGGCTGCGG
  AGCCCAACAACCACACGGAGTATGCCAGCATTCAGACCAGCCCGCAGCCCGCGTCGGAGG
  ACACCCTCACCTATGCTGACCTGGACATGGTCCACCTCAACCGGACCCCCAAGCAGCCGG
  CCCCCAAGCCTGAGCCGTCCTTCTCAGAGTACGCCAGCGTCCAGGTCCCGAGGAAGTGAA
  TGGGACCGTGGTTTGCTCTAGCACCCATCTCTACGCGCTTTCTTGTCCCACAGGGAGCCG
  CCGTGATGAGCACAGCCAACCCAGTTCCCGGAGGGCTGGGGCGGTGCAGGCTCTGGGACC
  CAGGGGCCAGGGTGGCTCTTCTCTCCCCACCCCTCCTTGGCTCTCCAGCACTTCCTGGGC
  AGCCACGGCCCCCTCCCCCCACATTGCCACATACCTGGAGGCTGACGTTGCCAAACCAGC
  CAGGGAACCAACCTGGGAAGTGGCCAGAACTGCCTGGGGTCCAAGAACTCTTGTGCCTCC
  GTCCATCACCATGTGGGTTTTGAAGACCCTCGACTGCCTCCCCGATGCTCCGAAGCCTGA
  TCTTCCAGGGTGGGGAGGAGAAAATCCCACCTCCCCTGACCTCCACCACCTCCACCACCA
  CCACCACCACCACCACCACCACTACCACCACCACCCAACTGGGGCTAGAGTGGGGAAGAT
  TTCCCCTTTAGATCAAACTGCCCCTTCCATGGAAAAGCTGGAAAAAAACTCTGGAACCCA
  TATCCAGGCTTGGTGAGGTTGCTGCCAACAGTCCTGGCCTCCCCCATCCCTAGGCTAAAG
  AGCCATGAGTCCTGGAGGAGGAGAGGACCCCTCCCAAAGGACTGGAGACAAAACCCTCTG
  CTTCCTTGGGTCCCTCCAAGACTCCCTGGGGCCCAACTGTGTTGCTCCACCCGGACCCAT
  CTCTCCCTTCTAGACCTGAGCTTGCCCCTCCAGCTAGCACTAAGCAACATCTCGCTGTGG
  ACGCCTGTAAATTACTGAGAAATGTGAAACGTGCAATCTTGAAACTGAGGTGTTAGAAAA
  CTTGATCTGTGGTGTTTTGTTTTGTTTTTTTTCTTAAAACAACAGCAACGTGATCTTGGC
  TGTCTGTCATGTGTTGAAGTCCATGGTTGGGTCTTGTGAAGTCTGAGGTTTAACAGTTTG
  TTGTCCTGGAGGGATTTTCTTACAGCGAAGACTTGAGTTCCTCCAAGTCCCAGAACCCCA
  AGAATGGGCAAGAAGGATCAGGTCAGCCACTCCCTGGAGACACAGCCTTCTGGCTGGGAC
  TGACTTGGCCATGTTCTCAGCTGAGCCACGCGGCTGGTAGTGCAGCCTTCTGTGACCCCG
  CTGTGGTAAGTCCAGCCTGCCCAGGGCTGCTGAGGGCTGCCTCTTGACAGTGCAGTCTTA
  TCGAGACCCAACGCCTCAGCCTGCTCATCCGTAAAGTGGGGATAGTGAAGATGACACCCC
  TCCCCACCACCTCTCATAAGCACTTTAGGAACACACAGAGGGTAGGGATAGTGGCCCTGG
  CCGTCTATCCTACCCCTTTAGTGACCGCCCCCATCCCGGCTTTCTGAGCTGATCCTTGAA
  GAAGAAATCTTCCATTTCTGCTCTCAAACCCTACTGGGATCAAACTGGAATAAATTGAAG
  ACAGCCAGGGGGATGGTGCAGCTGTGAAGCTCGGGCTGATTCCCCCTCTGTCCCAGAAGG
  TTGGCCAGAGGGTGTGACCCAGTTACCCTTTAACCCCCACCCTTCCAGTCGGGTGTGAGG
  GCCTGACCGGGCCCAGGGCAAGCAGATGTCGCAAGCCCTATTTATTCAGTCTTCACTATA
  ACTCTTAGAGTTGAGACGCTAATGTTCATGACTCCTGGCCTTGGGATGCCCAAGGGATTT
  CTGGCTCAGGCTGTAAAAGTAGCTGAGCCATCCTGCCCATTCCTGGAGGTCCTACAGGTG
  AAACTGCAGGAGCTCAGCATAGACCCAGCTCTCTGGGGGATGGTCACCTGGTGATTTCAA
  TGATGGCATCCAGGAATTAGCTGAGCCAACAGACCATGTGGACAGCTTTGGCCAGAGCTC
  CCGTGTGGCATCTGGGAGCCACAGTGACCCAGCCACCTGGCTCAGGCTAGTTCCAAATTC
  CAAAAGATTGGCTTGTAAACCTTCGTCTCCCTCTCTTTTACCCAGAGACAGCACATACGT
  GTGCACACGCATGCACACACACATTCAGTATTTTAAAAGAATGTTTTCTTGGTGCCATTT
  TCATTTTATTTTATTTTTTAATTCTTGGAGGGGGAAATAAGGGAATAAGGCCAAGGAAGA
  TGTATAGCTTTAGCTTTAGCCTGGCAACCTGGAGAATCCACATACCTTGTGTATTGAACC
  CCAGGAAAAGGAAGAGGTCGAACCAACCCTGCGGAAGGAGCATGGTTTCAGGAGTTTATT
  TTAAGACTGCTGGGAAGGAAACAGGCCCCATTTTGTATATAGTTGCAACTTAAACTTTTT
  GGCTTGCAAAATATTTTTGTAATAAAGATTTCTGGGTAATAATGAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC026692 unedited
  TACAGTTCGGATTTGTATACGACTCATATAGGCGGCCGCGTATTCCCGGGGTATCGTCGA
  CCCACGCGTCCGCGCCGTGTGGAGCCCGGGCGGGGCAGCCGCGGCCCATGGAGCCCGCCG
  GCCCGGCCCCCGGCCGCCTCGGGCCGCTGCTCTGCCTGCTGCTCGCCGCGTCCTGCGCCT
  GGTCAGGAGTGGCGGGTGAGGAGGAGCTGCAGGTGATTCAGCCTGACAAGTCCGTATCAG
  TTGCAGCTGGAGAGTCGGCCATTCTGCACTGCACTGTGACCTCCCTGATCCCTGTGGGGC
  CCATCCAGTGGTTCAGAGGAGCTGGACCAGCCCGGGAATTAATCTACAATCAAAAAGAAG
  GCCACTTCCCCCGGGTAACAACTGTTTCAGAGTCCACAAAGAGAGAAAACATGGACTTTT
  CCATCAGCATCAGTAACATCACCCCAGCAGATGCCGGCACCTACTACTGTGTGAAGTTCC
  GGAAAGGGAGCCCTGACACGGAGTTTAAGTCTGGAGCAGGCACTGAGCTGTCTGTGCGTG
  CCAAACCCTCTGCCCCCGTGGTATCGGGCCCTGCGGCGAGGGCCACACCTCAGCACACAG
  TGAGCTTCACCTGCGAGTCCCACGGCTTCTCACCCAGAGACATCACCCTGAAATGGTTCA
  AAAATGGGAATGAGCTCTCAGACTTCCAGACCAACGTGGACCCCGTAGGAGAGAGCGTGT
  CCTACAGCATCCACAGCACAGCCAAGGTGGTGCTGACCCGCGAGGACGTTCACTCTCAAG
  TCATCTGCGAGGTGGCCCACGTCACCTTGCAGGGGGACCCTCTTCGTGGGACTGCCCACT
  TGTCTGAGACCCTCCGAGTTCCACCCACCTTGGAGTTACTCACCGCCCCTGAGGGCAGAA
  AAACAG
  
• Restriction Sites: NotI-NotI
• ACCN: BC026692
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026692.1, AAH26692.1
• RefSeq Size: 3921 bp
• RefSeq ORF: 1521 bp
• Locus ID: 140885
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Gene Summary: The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]