GLUR3 (GRIA3) (NM_000828) Human Untagged Clone

CAT#: SC125535

GRIA3 (untagged)-Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLUR3
• Synonyms: GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC; MRX94; MRXSW
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000828, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAGGCAGAAGAAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGG
  GTCATTCTCACGGAGGATTCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACAGTGCAGGA
  GCACAGCGCTTTCCGCTTTGCCGTGCAGTTATACAACACCAACCAGAACACCACCGAGAAGCCCTTCCAT
  TTGAATTACCACGTAGATCACTTGGATTCCTCCAATAGTTTTTCCGTGACAAATGCTTTCTGCTCCCAGT
  TCTCGAGAGGGGTGTATGCCATCTTTGGATTCTATGACCAGATGTCAATGAACACCCTGACCTCCTTCTG
  TGGGGCCCTGCACACATCCTTTGTTACGCCTAGCTTCCCCACTGACGCAGATGTGCAGTTTGTCATCCAG
  ATGCGCCCAGCCTTGAAGGGCGCTATTCTGAGTCTTCTGGGTCATTACAAGTGGGAGAAGTTTGTGTACC
  TCTATGACACAGAACGAGGATTTTCCATCCTCCAAGCGATTATGGAAGCAGCAGTGCAAAACAACTGGCA
  AGTAACAGCAAGGTCTGTGGGAAACATAAAGGACGTCCAAGAATTCAGGCGCATCATTGAAGAAATGGAC
  AGGAGGCAGGAAAAGCGATACTTGATTGACTGCGAAGTCGAAAGGATTAACACAATTTTGGAACAGGTTG
  TGATCCTAGGGAAACACTCAAGAGGTTATCACTACATGCTCGCTAACCTGGGTTTTACTGATATTTTACT
  GGAAAGAGTCATGCATGGGGGAGCCAACATTACAGGTTTCCAGATTGTCAACAATGAAAACCCTATGGTT
  CAGCAGTTCATACAGCGCTGGGTGAGGCTGGATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTAA
  AGTATACATCTGCATTGACACACGACGCAATACTGGTCATAGCAGAAGCTTTCCGCTACCTGAGGAGGCA
  GCGAGTAGATGTGTCCCGGAGAGGAAGTGCTGGAGACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAA
  GGAATTGATATTGAGAGAGCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAATATTCAATTTGACA
  CTTATGGACGTAGGACAAATTATACCATCGATGTGTATGAAATGAAAGTCAGTGGCTCTCGAAAAGCTGG
  CTACTGGAATGAGTATGAAAGGTTTGTGCCTTTCTCAGATCAGCAAATCAGCAATGACAGTGCATCCTCA
  GAGAATCGGACCATAGTAGTGACTACCATTCTGGAATCACCATATGTAATGTACAAGAAGAACCATGAGC
  AACTGGAAGGAAATGAACGATATGAAGGCTATTGTGTAGACCTAGCCTATGAAATAGCCAAACATGTAAG
  GATCAAATACAAATTGTCCATCGTTGGTGACGGGAAATATGGTGCAAGGGATCCAGAGACTAAAATATGG
  AACGGCATGGTTGGGGAACTTGTCTATGGGAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGG
  TCCGTGAAGAAGTCATAGATTTTTCAAAGCCATTCATGAGCCTGGGCATCTCCATCATGATAAAGAAGCC
  TCAGAAATCAAAACCAGGCGTATTCTCATTTCTGGATCCCCTGGCTTATGAAATCTGGATGTGCATTGTC
  TTTGCTTACATTGGAGTCAGCGTAGTTCTTTTCCTAGTCAGCAGGTTCAGTCCTTATGAATGGCACTTGG
  AAGACAACAATGAAGAACCTCGTGACCCACAAAGTCCTCCTGATCCTCCAAATGAATTTGGAATATTTAA
  CAGTCTTTGGTTTTCCTTGGGTGCCTTTATGCAGCAAGGATGTGATATTTCTCCAAGATCACTCTCCGGG
  CGCATTGTTGGAGGGGTTTGGTGGTTCTTCACCCTGATCATAATTTCTTCCTATACTGCCAATCTCGCTG
  CTTTCCTGACTGTGGAGAGGATGGTTTCTCCCATAGAGAGTGCTGAAGACTTAGCTAAACAGACTGAAAT
  TGCATATGGGACCCTGGACTCCGGTTCAACAAAAGAATTTTTCAGAAGATCCAAAATTGCTGTGTACGAG
  AAAATGTGGTCTTACATGAAATCAGCGGAGCCATCTGTGTTTACCAAAACAACAGCAGACGGAGTGGCCC
  GAGTGCGAAAGTCCAAGGGAAAGTTCGCCTTCCTGCTGGAGTCAACCATGAATGAGTACATTGAGCAGAG
  AAAACCATGTGATACGATGAAAGTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAA
  GGCTCAGCATTAGGAAATGCTGTTAACCTGGCAGTATTAAAACTGAATGAGCAAGGCCTCTTGGACAAAT
  TGAAAAACAAATGGTGGTACGACAAAGGAGAGTGCGGCAGCGGGGGCGGTGACTCCAAGGACAAGACCAG
  CGCTCTGAGCCTGAGCAATGTGGCAGGCGTTTTCTATATACTTGTCGGAGGTCTGGGGCTGGCCATGATG
  GTGGCTTTGATAGAATTCTGTTACAAATCACGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCC
  AAAACTTTAAGCCTGCTCCTGCCACCAACACTCAGAATTATGCTACATACAGAGAAGGCTACAACGTGTA
  TGGAACAGAGAGTGTTAAGATCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000828 unedited
  TTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGAAGAGTGAGCGAGAGC
  AAGTTAAGGGGAGGGGGTGTAAGAGCCAGCGAATTCTTTTTCTTTTTCTATTATTATTTT
  GACGACTCCTGAGTTGCGCCCATGCTCTTGTCAGCTTCGTTTTAGGCGTAGCATGGCCAG
  GCAGAAGAAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCTGGGGCTTTTG
  GGTCATTCTCACGGAGGATTCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAAC
  ACAGTGCAGGAGCACAGCGCTTTCCGCTTTGCCGTGCAGTTATACAACACCAACCAGAAC
  ACCACCGAGAAGCCCTTCCATTTGAATTACCACGTAGATCACTTGGATTCCTCCAATAGT
  TTTTCCGTGACAAATGCTTTCTGCTCCCAGTTCTCGAGAGGGGTGTATGCCATCTTTGGA
  TTCTATGACCAGATGTCAATGAACACCCTGACCTCCTTCTGTGGGGCCCTGCACACATCC
  TTTGTTACGCCTAGCTTCCCCACTGACGCAGATGTGCAGTTTGTCATCCAGATGCGCCCA
  GCCTTGAAGGGCGCTATTCTGAGTCTTCTGGGTCATTACAAGTGGGGAGAAGTTTTGTGT
  ACCTCTATGACACAGAACGAGGATTTTCCATCCTCCAAGCGATTATGGAAGGCAGCAGTG
  CANAACAACTGGNCAGTAACAGCAAGGGNTCTGTGGGAAACATAAAAGGACGTCCAAGAA
  TTCAGGCGCATCATTGAAGAAATGGACAGGNAGCANNGAAAGCGATACTTTGATGACTTG
  CGAAGTCGAAAGGATTAACACATTNGGAACCGGGGTTGGGATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000828 unedited
  AAGGCCCTTTGNGGCAATTTTCTATTACAAAATGTATTAAAACTTTGTACAATACCTCAT
  GGCAATGAGGCTCTGTAGTTTATGCCCACACATGCAAATTACGCTTTTATAGCCATTAGT
  TTGCTTGGCAATTTTTCTGTTCACTGAAGTACAGCTTTAACAACAAAAAGAAAAGAATTG
  GAAAAAAAATCCTCAAGTGACTACTGCCAAACTCCCATAGCTTGAAGTTAGTAGCAGAAA
  TTTGAAGCAATCAACTTTTTTTTAAACATGCAAGTTTTTTTTAAAAAATAACAAACCAAT
  TTACCCGCCTCTTCATCTCAGTGACGTTTCCTCTCTGAAAAACTGATTTGAGTTAATAAA
  CATACATTTTTCTTTATACAGTGCCTATGAAAACATTTGTGTGTAGGTTGGTTGGTTTGT
  TTTATACAACTTCATGTTCACACAGCGTGAGCTTCCCGCGCTGGGACATTATAGCATTCA
  TAAGTTGAAAATCACAGTTTCATGAAAACAAAACAAAACAAAAACACAACAGCTTTCTTT
  TTTTGTTCAATACCATTTGAAATTTGACAAGTGAGTTGTTTGCTTATGACACCATTTTTG
  GAATGAAAGTTTATAGTACACTTTTCTGGAAACATTTCGTTGGAACGATACTTCTTCCTT
  TCTTTTCTGAAGACAAGAGCAAATATGCAGCGAGTCAACTAAGCATCTAAGGGTCACACT
  TGGGAAATGAATGCGAGCTCCTAGTTTTTGCAGTCTGGAAACTTCCCAATGCACGTTATC
  GATGAGAGCAAACTACTGAACTACAGGAAGCATACGTCGTTATATAGGAAGTAATTAGTG
  AGTGGAGACTATTTCTTTCGACTGTTCTGATGATGCCAACGGCNAGTATGCAGTTAGTTA
  CTTGATAGCCCATGGAGCANTGGAGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000828
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000828.2, NP_000819.1
• RefSeq Size: 5195 bp
• RefSeq ORF: 2685 bp
• Locus ID: 2892
• UniProt ID: P42263
• Cytogenetics: Xq25
• Domains: lig_chan, ANF_receptor
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Long-term depression, Neuroactive ligand-receptor interaction
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate internal coding exon compared to transcript variant 1, and encodes an isoform (2, also known as flop isoform) that is the same length, but with a few amino acid differences from isoform 1. RNA editing (AGA->GGA) changes Arg775Gly.