NLK (NM_016231) Human Untagged Clone

CAT#: SC124920

NLK (untagged)-Human nemo-like kinase (NLK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NLK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016231 edited
  ATGGCGGCTTACAATGGCGGTACATCTGCAGCAGCAGCAGGTCACCACCACCACCATCAC
  CACCACCTTCCACACCTCCCTCCTCCTCACCTGCACCACCACCACCACCCTCAACACCAT
  CTTCATCCGGGGTCGGCTGCCGCTGTACACCCTGTACAGCAGCACACCTCTTCGGCAGCT
  GCGGCAGCCGCAGCAGCGGCTGCAGCTGCAGCCATGTTAAACCCTGGGCAACAACAGCCA
  TATTTCCCATCACCGGCACCGGGGCAGGCTCCTGGACCAGCTGCAGCAGCCCCAGCTCAG
  GTACAGGCTGCCGCAGCTGCTACAGTTAAGGCGCACCATCATCAGCACTCGCATCATCCA
  CAGCAGCAGCTGGATATTGAGCCGGATAGACCTATTGGATATGGAGCCTTTGGTGTTGTC
  TGGTCAGTAACAGATCCAAGAGATGGAAAGAGAGTAGCGCTCAAAAAGATGCCCAACGTC
  TTCCAGAATCTGGTCTCTTGCAAAAGGGTCTTCCGGGAATTGAAGATGTTGTGTTTTTTT
  AAGCATGATAATGTACTCTCTGCCCTTGACATACTCCAACCTCCACACATTGACTATTTT
  GAAGAAATATATGTTGTCACAGAATTGATGCAGAGTGACCTACATAAAATTATCGTCTCT
  CCTCAACCACTCAGCTCAGATCATGTCAAAGTTTTTCTTTATCAGATTTTGCGAGGTTTG
  AAATATCTCCATTCAGCTGGCATTTTACATCGAGACATTAAGCCAGGGAATCTCCTTGTG
  AACAGCAACTGTGTTCTAAAGATTTGTGATTTTGGATTGGCCAGAGTGGAAGAATTAGAT
  GAATCCCGTCATATGACTCAGGAAGTTGTTACTCAGTATTATCGGGCTCCAGAAATCCTG
  ATGGGCAGCCGTCATTACAGCAATGCTATTGACATCTGGTCTGTGGGATGTATCTTTGCA
  GAACTACTAGGACGAAGAATATTGTTTCAGGCACAGAGTCCCATTCAGCAGTTGGATTTG
  ATCACGGATCTGTTGGGCACACCATCACTGGAAGCAATGAGGACAGCTTGTGAAGGCGCT
  AAGGCACATATACTCAGGGGTCCTCATAAACAGCCATCTCTTCCTGTACTCTATACCCTG
  TCTAGCCAGGCTACACATGAAGCTGTTCATCTCCTTTGCAGGATGTTGGTCTTTGATCCA
  TCCAAAAGAATATCCGCTAAGGATGCCTTAGCCCACCCCTACCTAGATGAAGGGCGACTA
  CGATATCACACATGTATGTGTAAATGTTGCTTTTCCACCTCCACTGGAAGAGTTTATACC
  AGTGACTTTGAGCCTGTCACCAATCCCAAATTTGATGACACTTTCGAGAAGAACCTCAGT
  TCTGTCCGACAGGTTAAAGAAATTATTCATCAGTTCATTTTGGAACAGCAGAAAGGAAAC
  AGAGTGCCTCTCTGCATCAACCCTCAGTCTGCTGCTTTTAAGAGCTTTATTAGTTCCACT
  GTTGCTCAGCCATCTGAGATGCCCCCATCTCCTCTGGTGTGGGAGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016231 unedited
  GTAGACCTTGTATACGACCTCCTATAGGGCGGCCGCGATCCTCGGCACCAGGCTTGACCC
  AGTTTGCTTTCCAATCAAAGGGCATTTATTTTGAATGTCTCTTTGTGGCGCAAGAGCCAA
  CGCAAAAATGATGGCGGCTTACAATGGCGGTACATCTGCAGCAGCAGCAGGTCACCACCA
  CCACCATCACCACCACCTTCCACACCTCCCTCCTCCTCACCTGCACCACCACCACCACCC
  TCAACACCATCTTCATCCGGGGTCGGCTGCCGCTGTACACCCTGTACAGCAGCACACCTC
  TTCGGCAGCTGCGGCAGCCGCAGCAGCGGCTGCAGCTGCAGCCATGTTAAACCCTGGGCA
  ACAACAGCCATATTTCCCATCACCGGCACCGGGGCAGGCTCCTGGACCAGCTGCAGCAGC
  CCCAGCTCAGGTACAGGCTGCCGCAGCTGCTACAGTTAAGGCGCACCATCATCAGCACTC
  GCATCATCCACAGCAGCAGCTGGATATTGAGCCGGATAGACCTATTGGATATGGAGCCTT
  TGGTGTTGTCTGGTCAGTAACAGATCCAAGAGATGGAAAGAGAGTAGCGCTCAAAAAGAT
  GCCCAACGTCTTCCAGAATCTGGTCTCTTGCAAAAGGGTCTTCCGGGAATTGAAGATGTT
  GTGTTTTTTTAAGCATGATAATGTACTCTCTGCCCTTGACATACTCCAACCTCCACACAT
  TGACTATTTTGAAGAAATATATGTTGTCACAGAATTGATGCAGAGTGACCTACATAAAAT
  TATCGTCTCTCCTCAACCACTCAGCTCAGATCATGTCAAGTTTTCTTTATCAGATTTTGC
  GAGGTTTGAAATATCTCCATTCAGCTGGCATTTACATCGAGACATTAAGCCAGGGAATCC
  TCCTTGTGAACAGCAACTGGTGTTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016231 unedited
  CTCCCAGTCCTCCGTGGAAGCTCATTACATCTTCGTATTACATTATCTTCCACCATCACT
  CCCACACCAGAGGAGATGGGGGCATCTCAGATGGCTGAGCAACAGTGGAACTAATAAAGC
  TCTTAAAAGCAGCAGACTGAGGGTTGATGCAGAGAGGCACTCTGTTTCCTTTCTGCTGTT
  CCAAAATGAACTGATGAATAATTTCTTTAACCTGTCGGACAGAACTGAGGTTCTTCTCGA
  AAGTGTCATCAAATTTGGGATTGGTGACAGGCTCAAAGTCACTGGTATAAACTCTTCCAG
  TGGAGGTGGAAAAGCAACATTTACACATACATGTGTGATATCGTAGTCGCCCTTCATCTA
  GGTAGGGGTGGGCTAAGGCATCCTTAGCGGATATTCTTTTGGATGGATCAAAGACCAACA
  TCCTGCAAAGGAGATGAACAGCTTCATGTGTAGCCTGGCTAGACAGGGTATAGAGTACAG
  GAAGAGATGGCTGTTTATGAGGACCCCTGAGTATATGTGCCTTAGCGCCTTCACAAGCTG
  TCCTCATTGCTTCCAGTGATGGTGTGCCCAACAGATCCGTGATCAAATCCAACTGCTGAA
  TGGGACTCTGTGCCTGAAACAATATTCTTCGTCCTAGTAGTTCTGCAAAGATACATCCCA
  CAGACCAGATGTCAATAGCATTGCTGTAATGACGGCTGCCCATCAGATTTCTGGAGCCCG
  ATAATACTGAGTAACAACTTCCTGAGTCATATGACGGGATTCATCTAATTCTTCCACTCT
  GGCCAATCCAAAATCACAAATCTTTAGAACACAGTTGCTGTTCACAAGGAGATTCCCTGG
  CTTAATGTCTCGATGTAAAATGCCAGCTGATGGAGATATTTCAACCTCGCAAAATCTGAT
  AAGAAAAACCTTTGACATGATCTGAACTGATGGTTGAGGAGAGACGATTATTTATGGTAG
  GGTACAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016231
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016231.2, NP_057315.2
• RefSeq Size: 4061 bp
• RefSeq ORF: 3555 bp
• Locus ID: 51701
• UniProt ID: Q9UBE8
• Cytogenetics: 17q11.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Adherens junction, MAPK signaling pathway, Wnt signaling pathway
• Gene Summary: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).[UniProtKB/Swiss-Prot Function]