CD57 (B3GAT1) (NM_054025) Human Untagged Clone

CAT#: SC124786

B3GAT1 (untagged)-Human beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3GAT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD57
• Synonyms: CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_054025, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGAAGAGACGGGACATCCTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTCT
  GGCACCAGAGCACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCCCGACGCGAAAC
  GCCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATCGTGGAGGTGGTGCGCACCGAG
  TACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTGCCCACCATCCACGTGGTGACGCCCACCTACA
  GCCGCCCGGTGCAGAAGGCCGAGCTGACGCGCATGGCCAACACGCTGCTGCACGTGCCCAACCTCCACTG
  GCTGGTGGTGGAGGATGCGCCGCGCCGGACGCCGCTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAAC
  TACACGCACCTGCACGTGGAGACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATCC
  CGCGGGGCACCATGCAGCGCAACCTGGCCCTGCGCTGGCTGCGCGAGACCTTCCCGCGCAACTCCAGCCA
  GCCTGGCGTGGTCTACTTCGCCGACGACGACAACACCTACAGCCTGGAGCTCTTCGAAGAGATGCGCAGC
  ACCAGGAGGGTGTCCGTGTGGCCCGTCGCCTTCGTGGGTGGCCTGCGGTACGAGGCCCCACGGGTGAACG
  GGGCAGGGAAGGTGGTCGGCTGGAAGACGGTGTTTGACCCCCACCGGCCATTTGCAATAGACATGGCTGG
  ATTTGCCGTCAACCTGCGGCTCATTCTGCAGCGAAGCCAGGCCTACTTCAAGCTGCGAGGTGTGAAGGGA
  GGCTACCAGGAAAGCAGCCTCCTTCGAGAACTTGTCACCCTCAACGACCTGGAGCCCAAGGCAGCCAACT
  GCACCAAGATCCTGGTGTGGCACACACGGACAGAGAAGCCAGTGCTGGTGAATGAGGGCAAGAAGGGCTT
  CACTGACCCCTCGGTGGAGATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_054025 unedited
  TGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACCCCCCAGGTTCC
  TGACCCCTGCCCCTGGACAGCGACCCCTTCTCAGACTCCAGTTGGGCCGGACTCTCCAAA
  CCTGCTTCCGCAATGGGTAATGAGGAGCCGTGGGTGCAGCCAGCCTTGGAGATGCCGAAG
  AGACGGGACATCCTAGCGATCGTCCTCATCGTGCTGCCCTGGACTCTGCTCATCACTGTC
  TGGCACCAGAGCACCCTCGCACCCCTGCTCGCGGTACATAAGGATGAGGGCAGTGACCCC
  CGACGCGAAACGCCGCCCGGCGCCGACCCCAGGGAGTACTGCACGTCTGACCGCGACATC
  GTGGAGGTGGTGCGCACCGAGTACGTGTACACGCGGCCCCCGCCATGGTCCGACACGCTG
  CCCACCATCCACGTGGTGACGCCCACCTACAGCCGCCCGGTGCAGAAGGCCGAGCTGACG
  CGCATGGCCAACACGCTGCTGCACGTGCCCAACCTCCACTGGCTGGTGGTGGAGGATGCG
  CCGCGCCGGACGCCGTTGACCGCGCGCCTGCTGCGCGACACCGGCCTCAACTACACGCAC
  CTGCACGTGGAGACGCCCCGCAACTACAAGCTGCGCGGAGACGCCCGCGACCCACGCATC
  CCGCGGGGCACCATGCAGCGCAAACCTGGCCCTGCGCTGGCTGGGCGAGACCTTCCGCGC
  AACTCCACCAGCTTGGGGTGGTCTACTTTGCCGACGACAACAAAACTTAAGCCTGGAACT
  TTTNAAGAGATGCCCCACACCAGGAGGGGTCCCGGGGGGCCCGTCCCCTTCGGGGTGGCC
  CGGGGAACAAGCCCCAACGGTGAACCGGGCATGGTAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_054025
• Insert Size: 3620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_054025.1, NP_473366.1
• RefSeq Size: 3666 bp
• RefSeq ORF: 3666 bp
• Locus ID: 27087
• UniProt ID: Q9P2W7
• Cytogenetics: 11q25
• Domains: Glyco_transf_43
• Protein Families: Transmembrane
• Protein Pathways: Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has multiple differences in the 5' end of the transcript, as compared to variant 1. Variants 1 and 2 encode the same protein.