ST3GAL3 (NM_174964) Human Untagged Clone

CAT#: SC124687

ST3GAL3 (untagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 3 (ST3GAL3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST3GAL3
• Synonyms: DEE15; EIEE15; MRT12; SIAT6; ST3GALII; ST3Gal III; ST3GalIII; ST3N
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124687 sequence for NM_174964 edited (data generated by NextGen Sequencing)
  ATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTG
  GGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAGT
  AAGTATAGTCACTCTAGCTCACCCCAGGAGAAGCCTGTTGCAGATTCAGTGGTTCTTTCC
  TTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAAT
  CTGGACTCTAAACTGCCTGCTGAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCT
  TGCAAGCCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCA
  GCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCG
  CCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCAGTCACCAAAGAG
  TACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCTGCATCATCGTGGGCAAT
  GGAGGCGTTCTTGCCAACAAGTCTCTGGGGTCACGAATTGACGACTATGACATTGTGGTG
  AGACTGAATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTG
  CGCATCACCTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTC
  TTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATACATCGTCTACAAG
  GAGAGAGTGAGTGCATCGGATGGCTTCTGGAAATCTGTGGCCACTCGAGTGCCCAAGGAG
  CCCCCTGAGATTCGAATCCTCAACCCATATTTCATCCAGGAGGCCGCCTTCACCCTCATT
  GGCCTGCCCTTCAACAATGGCCTCATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTG
  GCAGTGACCATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAGGATTTGGCTATGAC
  ATGAGCACACCCAACGCACCCCTGCACTACTATGAGACCGTTCGCATGGCAGCCATCAAA
  GAGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCT
  CGCGTCATCACTGATCTAAGCAGTGGCATCTGA
  
  Clone variation with respect to NM_174964.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_174964 unedited
  AGGAATTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACCAGGCCCCGCGTC
  GGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAA
  ATCATGTGAAGATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCT
  TTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGG
  AGGACTCCAATTCAGTGGTTCTTTCCTTTGACTNNCGCTGACAACACTAAGCTCAAAAAT
  TGAACCGGGGGGGCCTTCCCCCCGAAAAAGGGGGCCTTAAAAACCGCGCGGGGGGTAACC
  CCCCCCCCCCCCCCCCCCTCTTTTTTAAAGAGAGGGTGGGCCCCCCCCCCGTTTTTTTTC
  TCCCCCGGGAGAAAGAGAGAATTTTTCTCCCCGGGGGGGAACAACCACCCCCCCCACCCC
  CGCCGGGGGGGGAAGAAAAATTTTTTCCNTCAAAAAAAAAAAAAAAAAAAAAAAAAAACC
  CCCCCCCCTCTTTTTTTAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAATATTTTTTTTTT
  TTTTAAAAAAAAAAAAAAAANTTTTTNCCCCCCTTTCTTTTCTTTTCTTTTNTNNNNNTT
  TTTTTTTTTTTTGGGGTGGGGGGGGGGGGGGGCGGCGGCCCCCCCCCCCCCCCCGGAGCN
  CCCCGCCTCCCCTCTTCTTTTTTTTGGGGAGAGAAAAAAACTCACCCCCNCGGCGGGAGG
  GGGGGGGGGGGGGGGAAAAAAGAGNATATNNTNCCTCTCTTCCCCCCTCCGCCCCCGGGG
  GGGGGGGGGGGGGNNNACCCCNCNNCCNNNNNNATTTTTTTTTTTTTTTTTTTNNNNGAG
  AATAAAAAAAAAAAAAAAAAAAGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_174964 unedited
  NNNTTTTTACTNTGNACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTAAGGAAAAAGCTTTATTGTTTCCATGGGGGGGGCCTGG
  AACGGTTGCCACAGTTGGGGAAACCTCTTTGGGCCTCATTTCCTTTTGGCCCAGGTTAAA
  GGCAAAACCCAACCCCCTGGCAGTTTTGTTGTTAAAACCTAAAACATGTGGAAAGTTGGG
  AAGTCCGGCCCTGGGGTATTCCTATGCATAACTTGCAGTTGGGGAGGGGAGCTTTTCCCA
  GCAGGGGGAGCTGGGCGTCACCCTCCTGAGCTTTAAAGTTTTTTTTGTTATACCCCTGGG
  GCGGTTTTGTTGGCTCCAAAGGGATGGCTCCAGGGTTTCCCCATGGAACAGTTAGGGCTG
  GGGAGGGGAAATACCCCGCCTTCTCCCCAAAAAAGGCCAAACAGTGTCATGACCTGGAGG
  GGGCTGGTTTGAAATCCTGCCCACCATCCTGTACCCCCACAGAAAGGTACAGGCTGGTCC
  TGGCACTCCTGCCCCACAGGGAGCTAACAGGTAACACTGCCTGATGGGCCCTTTAGGGGG
  CCTTTTCCTGCATGGCAAGTGAACCCAACTTGACATCTCCAACCTTATTAAAAAATAAAT
  TATCCCTACCCTCCCCCCTCCCCAAATTCACAAAATGATATTCCCCCAAACACTGATTGG
  AAAAGTGATTCCGGAAGAAGCCTTGCTGCCCAGGGTCCCACCACCTGCATGCTGGGCCTG
  CTGGGCAAGCCAGAACCCTGCTGGCCCTCTGCATCAAGGACCCATGCTGAAGAGGCTGAN
  TGCTGGTCTCTGAAATGGCTGTTAACCAGGCACACTTTGGCTGCTCACCAAGGCCTGCTG
  CCCCTGGGCCCTTGCCACGTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_174964
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174964.1, NP_777624.1
• RefSeq Size: 2316 bp
• RefSeq ORF: 1173 bp
• Locus ID: 6487
• UniProt ID: Q11203
• Cytogenetics: 1p34.1
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Mutations in this gene have been associated with a form of autosomal recessive nonsymdromic cognitive disability as well as infantile epileptic encephalopathy. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1, resulting in an isoform (b, also called A1) that is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.