CACNB2 (NM_000724) Human Untagged Clone

CAT#: SC124059

CACNB2 (untagged)-Human calcium channel, voltage-dependent, beta 2 subunit (CACNB2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNB2
• Synonyms: CAB2; CACNLB2; CAVB2; MYSB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124059 sequence for NM_000724 edited (data generated by NextGen Sequencing)
  ATGCAGTGCTGCGGGCTGGTGCATCGCCGGCGAGTACGGGTGTCCTATGGTTCGGCAGAC
  TCCTACACTAGCCGTCCATCCGATTCCGATGTATCTCTGGAGGAGGACCGGGAGGCAGTG
  CGCAGAGAAGCGGAGCGGCAGGCCCAGGCACAGTTGGAAAAAGCAAAGACAAAGCCCGTT
  GCATTTGCGGTTCGGACAAATGTCAGCTACAGTGCGGCCCATGAAGATGATGTTCCAGTG
  CCTGGCATGGCCATCTCATTCGAAGCAAAAGATTTTCTGCATGTTAAGGAAAAATTTAAC
  AATGACTGGTGGATAGGGCGATTGGTAAAAGAAGGCTGTGAAATCGGATTCATTCCAAGC
  CCAGTCAAACTAGAAAACATGAGGCTGCAGCATGAACAGAGAGCCAAGCAAGGGAAATTC
  TACTCCAGTAAATCAGGAGGAAATTCATCATCCAGTTTGGGTGACATAGTACCTAGTTCC
  AGAAAATCAACACCTCCATCATCTGCTATAGACATAGATGCTACTGGCTTAGATGCAGAA
  GAAAATGATATTCCAGCAAACCACCGCTCCCCTAAACCCAGTGCAAACAGTGTAACGTCA
  CCCCACTCCAAAGAGAAAAGAATGCCCTTCTTTAAGAAGACAGAGCACACTCCTCCGTAT
  GATGTGGTACCTTCCATGCGACCAGTGGTCCTAGTGGGCCCTTCTCTGAAGGGCTACGAG
  GTCACAGATATGATGCAAAAAGCGCTGTTTGATTTTTTAAAACACAGATTTGAAGGGCGG
  ATATCCATCACAAGGGTCACCGCTGACATCTCGCTTGCCAAACGCTCGGTATTAAACAAT
  CCCAGTAAGCACGCAATAATAGAAAGATCCAACACAAGGTCAAGCTTAGCGGAAGTTCAG
  AGTGAAATCGAAAGGATTTTTGAACTTGCAAGAACATTGCAGTTGGTGGTCCTTGACGCG
  GATACAATTAATCATCCAGCTCAACTCAGTAAAACCTCCTTGGCCCCTATTATAGTATAT
  GTAAAGATTTCTTCTCCTAAGGTTTTACAAAGGTTAATAAAATCTCGAGGGAAATCTCAA
  GCTAAACACCTCAACGTCCAGATGGTAGCAGCTGATAAACTGGCTCAGTGTCCTCCAGAG
  CTGTTCGATGTGATCTTGGATGAGAACCAGCTTGAGGATGCCTGTGAGCACCTTGCCGAC
  TATCTGGAGGCCTACTGGAAGGCCACCCATCCTCCCAGCAGTAGCCTCCCCAACCCTCTC
  CTTAGCCGTACATTAGCCACTTCAAGTCTGCCTCTTAGCCCCACCCTAGCCTCTAATTCA
  CAGGGTTCTCAAGGTGATCAGAGGACTGATCGCTCCGCTCCTATCCGTTCTGCTTCCCAA
  GCTGAAGAAGAACCTAGTGTGGAACCAGTCAAGAAATCCCAGCACCGCTCTTCCTCCTCA
  GCCCCACACCACAACCATCGCAGTGGGACAAGTCGCGGCCTCTCCAGGCAAGAGACATTT
  GACTCGGAAACCCAGGAGAGTCGAGACTCTGCCTACGTAGAGCCAAAGGAAGATTATTCC
  CATGACCACGTGGACCACTATGCCTCACACCGTGACCACAACCACAGAGACGAGACCCAC
  GGGAGCAGTGACCACAGACACAGGGAGTCCCGGCACCGTTCCCGGGACGTGGATCGAGAG
  CAGGACCACAACGAGTGCAACAAGCAGCGCAGCCGTCATAAATCCAAGGATCGCTACTGT
  GAAAAGGATGGAGAAGTGATATCAAAAAAACGGAATGAGGCTGGGGAGTGGAACAGGGAT
  GTTTACATCCGCCAATGA
  
  Clone variation with respect to NM_000724.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000724 unedited
  GGCGCGATACATTCTGACACCGGACAAATCAAATTGCCCAAAAGTTACAGAGGTAACAGT
  GAATCCAGGATGCTGCTATCAGCCTGTGCATTGTGAAGAAGGCAATCATAGGCGAGCAGC
  CGCGGGAGCAGGAACAGCAGCGTGCTAAGAAGCAGTCACATAAACAGCAGCAGGAGTATG
  TTTTCTGCTTTTCAAAAGCAGAGTACTGCAGGGTCGCGAAATGCAAGACACTCAGATGTT
  TGAAAATCTCCCGAGTTGAGAATGGCTACTGTAAAAGCGTCACCAAGAAACTCTGACGAT
  CTGGACAGTCCTAACTCTGTGTTAGCAATACTTACTTCCGGAAAATTAATGCTACTTCTT
  GTAGATTTTTGCAAATAGGAAACCCCCTTGAAGAAGATCTCAAATTACGCCCCCCACCCC
  CAAAAAAAGACAAACAGGGGAGAACAAAGTTTTGGCATGCCTGCAGGAACGGTGGCTTTT
  TTAGAAACTACCTAGGAGGCAGAAGCTAAGTGATTTGCTCATGCCTCTTACCTGGGAGTA
  GAAGGTGGGAAGAAATGGACCGAGGCTGTGACGAGAAGACAAGGCACAGTGCAGCTTGGT
  GAAGCCACACGCTGACTGCGTTCTGCCCCCTCTTCATGCAGTGCTGCGGGCTGGTGCATC
  GCCGGCGAGTACGGGTGTCCTATGGTTCGGCAGACTCCTACACTAGCCGTCCATCCGATT
  CCGATGTATTTCTGGAGGAGGACCGGGAGGCAGTGCGCATAGAACCGGAGCGGCAGGCCC
  AGGCCAGTTGGTAAAAGCAAAGACCAACCCGTTGGCATTGCGGTTCGGACAAATGTCAGC
  TACCGTGCGGCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000724 unedited
  CCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAG
  CTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAGTGGGAGAA
  GTTATAAAAATTTATTTTCCCACGAAATAATTCCACAAGCTGTGTAAACAAACATTTCTT
  ACTAAAACAAATACTCTAACACACAAAGATTGTACATTTCCAGTGAAACACTCACAGTAA
  AATTGCCTGTATTTTCACTCTCTAGGAGCAAAAACAAGGAGGCAGCAGATGAATGCTTTC
  CTAGTTACCTTTGGCAGCAAGCTTACTCACATTCCCTGACTGCTCAATGGGTTGTTAAAG
  CAACATATAGCACAATTAATTAAATACCGGACCGAGGTGGGCAGAATGCTCGATATCAGT
  CTCTAGTTTCTTCGTTAGTCTTCAGCATTCCAATTCATCTCAACCATTCAGTCACTCGGT
  GCACACTTTCTTACATACGGACACACACACGTGCATATAGCGAGTTCCGGCTCGTAGACT
  CCAGTGCAGTGACTAGAACAGACACTAGCTACTAAGAATGTGTCACAATCAGTGTGAACT
  GTATGGAGTAGAACAACCTCTGGAGTTNTATATTTACCATAATTTATGGTGGAAATTACA
  AACTTACTTANCAGGATGGTATAACATTCAGAATTCAAACCTTTTTCAGTCAAGCAATCA
  CCCATATTACAGTATGTACACACTTTCTACTAGATTGAGGAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000724
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000724.2, NP_000715.1
• RefSeq Size: 3721 bp
• RefSeq ORF: 1818 bp
• Locus ID: 783
• UniProt ID: Q08289
• Cytogenetics: 10p12.33-p12.31
• Domains: Ca_channel_B, SH3, GuKc
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• Gene Summary: This gene encodes a subunit of a voltage-dependent calcium channel protein that is a member of the voltage-gated calcium channel superfamily. The gene product was originally identified as an antigen target in Lambert-Eaton myasthenic syndrome, an autoimmune disorder. Mutations in this gene are associated with Brugada syndrome. Alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Feb 2013]
  Transcript Variant: This variant (1) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 2. These differences cause translation initiation at an alternate start codon, and result in an isoform (1) with a shorter and distinct N-terminus, compared to isoform 2.