GRID2 (NM_001510) Human Untagged Clone

CAT#: SC123987

GRID2 (untagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRID2
• Synonyms: GluD2; SCAR18
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001510, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGTTTTCCCCTTTCTCTTGGTTTTGTCCGTCTGGTGGTCTCGAACCTGGGACTCGGCGAATGCGG
  ATTCGATCATTCACATCGGAGCAATTTTTGATGAATCTGCCAAAAAGGATGATGAGGTATTTCGCACTGC
  GGTTGGTGACCTTAACCAGAATGAGGAGATCTTACAGACTGAGAAAATCACATTTTCAGTGACGTTTGTT
  GATGGCAACAACCCTTTCCAAGCAGTTCAAGAAGCCTGTGAACTTATGAATCAAGGCATCTTGGCCCTGG
  TCAGCTCCATTGGCTGCACGTCAGCAGGATCCCTCCAGTCTTTGGCAGACGCCATGCATATCCCCCACCT
  CTTCATTCAGCGCTCAACAGCTGGGACCCCAAGGAGTGGCTGTGGACTCACCCGGAGCAACAGGAATGAT
  GACTACACTCTCTCAGTTCGCCCACCTGTCTACTTGCATGATGTTATCCTAAGAGTGGTCACAGAGTATG
  CCTGGCAGAAATTCATTATATTCTATGATAGTGAATACGATATCCGTGGAATACAGGAGTTCTTGGACAA
  AGTCTCTCAGCAGGGAATGGATGTTGCACTTCAGAAGGTAGAAAACAACATCAATAAAATGATTACCACT
  CTCTTTGACACCATGAGAATAGAAGAACTGAATCGCTATCGAGACACTCTTAGGCGAGCGATCCTTGTTA
  TGAATCCTGCTACAGCCAAATCCTTCATTACTGAGGTTGTGGAGACTAATTTGGTTGCTTTTGACTGTCA
  CTGGATCATTATAAATGAGGAAATAAACGATGTGGACGTACAGGAACTTGTAAGAAGGTCAATTGGAAGG
  TTAACGATTATTCGGCAGACATTTCCAGTTCCCCAGAACATAAGTCAGCGGTGTTTCCGTGGCAACCATC
  GAATATCTTCAACATTGTGTGATCCAAAGGATCCATTTGCTCAGAATATGGAGATTTCCAACCTTTACAT
  ATATGACACGGTGCTTCTGCTTGCTAATGCTTTTCATAAGAAGCTGGAGGACCGAAAGTGGCACAGCATG
  GCAAGTCTGTCATGTATCAGAAAGAACTCAAAGCCCTGGCAGGGTGGGCGCTCCATGTTGGAGACCATCA
  AGAAGGGTGGAGTTAGTGGGTTGACTGGAGAGCTAGAATTTGGAGAAAATGGAGGCAATCCCAATGTCCA
  CTTTGAAATCCTTGGAACCAACTATGGAGAAGAGCTTGGCAGAGGTGTTCGAAAACTTGGTTGCTGGAAT
  CCTGTCACAGGTCTGAATGGGTCACTGACTGACAAGAAATTGGAGAATAACATGCGTGGAGTGGTTCTAC
  GTGTAGTAACTGTTCTGGAAGAACCTTTTGTGATGGTCTCTGAAAATGTCTTGGGTAAGCCGAAGAAATA
  CCAGGGCTTCTCCATTGATGTTTTGGATGCCTTATCTAACTACCTGGGTTTTAACTACGAAATTTACGTA
  GCACCGGATCACAAATACGGAAGCCCACAAGAAGATGGGACATGGAATGGCTTGGTAGGAGAACTTGTCT
  TTAAGAGAGCCGACATAGGGATTTCTGCTTTAACCATCACTCCAGATCGTGAAAATGTGGTGGACTTTAC
  GACACGTTACATGGACTACTCAGTGGGGGTACTACTTCGAAGGGCTGAAAAGACAGTGGATATGTTTGCC
  TGTCTTGCACCATTTGATCTCTCTCTATGGGCTTGCATTGCTGGCACAGTCCTTCTGGTGGGTCTACTGG
  TCTACCTCTTGAACTGGCTTAATCCCCCACGATTACAAATGGGATCAATGACGTCTACTACTCTCTACAA
  CTCCATGTGGTTTGTGTATGGATCTTTTGTACAACAAGGCGGGGAAGTCCCGTACACGACTCTGGCTACC
  CGAATGATGATGGGGGCTTGGTGGCTATTTGCTTTGATTGTTATCTCATCTTACACGGCAAACCTCGCTG
  CTTTCCTCACTATTACACGCATTGAAAGTTCCATCCAGTCTCTCCAGGACCTTTCCAAGCAAACAGAAAT
  CCCTTATGGCACAGTCCTAGACTCTGCGGTATATGAGCATGTCCGCATGAAAGGACTGAATCCTTTTGAG
  AGGGACAGCATGTATTCCCAAATGTGGCGGATGATCAACCGAAGCAATGGATCGGAGAACAATGTTCTGG
  AGTCCCAGGCAGGCATTCAAAAGGTAAAATATGGAAATTATGCTTTCGTATGGGATGCAGCTGTATTGGA
  ATATGTGGCTATCAATGACCCAGATTGTTCCTTTTACACCATTGGAAATACTGTTGCTGATCGGGGATAT
  GGAATTGCATTACAACATGGCAGTCCTTACCGAGATGTTTTTTCACAAAGGATCCTGGAGCTTCAGCAGA
  ATGGTGACATGGACATCCTGAAGCACAAATGGTGGCCTAAGAATGGCCAGTGTGACCTGTACTCGTCAGT
  GGACACAAAGCAGAAAGGAGGCGCCCTGGACATAAAGAGCTTTGCAGGGGTCTTTTGTATCCTGGCTGCT
  GGAATTGTCCTCTCCTGCTTCATAGCCATGCTGGAGACGTGGTGGAACAAGAGGAAAGGCTCCCGGGTTC
  CATCAAAAGAGGATGACAAGGAAATTGACCTGGAGCACCTCCATAGACGTGTAAATAGCTTGTGCACAGA
  TGACGACAGCCCCCATAAACAGTTTTCCACCTCGTCAATTGATTTGACCCCTCTGGACATTGACACTTTG
  CCAACACGACAAGCACTGGAGCAAATCAGTGATTTCAGGAACACTCATATTACCACAACAACCTTTATCC
  CAGAGCAGATCCAGACTCTTAGCCGCACACTGTCAGCTAAAGCTGCTTCTGGTTTCACTTTTGGCAACGT
  GCCTGAGCACCGAACTGGCCCTTTTAGGCACAGGGCACCTAATGGGGGCTTTTTCAGGAGTCCTATAAAA
  ACAATGTCATCTATTCCTTATCAACCAACTCCTACCCTGGGGCTCAATCTGGGTAATGATCCAGACCGAG
  GCACCTCCATATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001510 unedited
  ATTTTTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCAAACTCTTTGG
  ACTGTTTGAAAAAAAAAAAAATTGGAAGAAAATCCATCCTCCAAGAGAATCGGCGTAGGA
  GGAGATGGAAGTTTTCCCCTTTCTCTTGGTTTTGTCCGTCTGGTGGTCTCGAACCTGGGA
  CTCGGCGAATGCGGATTCGATCATTCACATCGGAGCAATTTTTGATGAATCTGCCAAAAA
  GGATGATGAGGTATTTCGCACTGCGGTTGGTGACCTTAACCAGAATGAGGAGATCTTACA
  GACTGAGAAAATCACATTTTCAGTGACGTTTGTTGATGGCAACAACCCTTTCCAAGCAGT
  TCAAGAAGCCTGTGAACTTATGAATCAAGGCATCTTGGCCCTGGTCAGCTCCATTGGCTG
  CACGTCAGCAGGATCCCTCCAGTCTTTGGCAGACGCCATGCATATCCCCCACCTCTTCAT
  TCAGCGCTCAACAGCTGGGACCCCAAGGAGTGGCTGTGGACTCACCCGGAGCAACAGGAA
  TGATGACTACACTCTCTCAGTTCGCCCACCTGTCTACTTGCATGATGTTATCCTAAGAGT
  GGTCACAGAGTATGCCTGGCAGAAATTCATTATATTCTATGATAGTGAATACGATATCCG
  TGGAATACAGGAGTTCTGGNACAAAGTCTCTCCAGGAACTTTTCCAGCANACAGAANATC
  CTTATTGCACAGTCCTAGACTCTGCGGTATATGAGCATGTCCGCTGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001510 unedited
  CATGGCACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTGGGGTT
  TTTTTTTTTTTTTTAAAAAACATTTTTATTATACAAATTATTTAAACAAATTATACCCAG
  AAAGCTCTGTCACTCATAACTGAGGCCTCCAAAAATGTTTTGTGTGTTTGTAAATAGGCG
  ATACATCAAAATAAGCCTATTTTAAATATTGTACATGGTTAACAGTTTGTTGATAGCTAA
  AATCACTGCAACATCTGGTATGTCCCTATATAGCTTGGTGTAAAGATTTTTTTGTTTTAA
  AATCAAAGTTGTAAATGAGATCATGAATGTTACAAGGGAAAACAAGTTGTGCATGCACTT
  AGTTACTTGTTACCCTGGAAACAAAATGGTTTTTGCATGATAAGAAATTTTTCTGCCTTA
  CACAGTAGACAATATTTGAAGATTTAGTACAAAACAGTGACAATACAAGATGCTCCCCAG
  AACAGAATTAGCATCAAAATATAATATATCTTAAGAATATACCTTTTAATTTCTCCTAAA
  GCAAATCACATAGGTTGATTCCTATATCAATCCTAACCACAGAATGAGTTAGAATAAAGT
  AAGTGTATGGCTGCCTATAAACACAAATNAATTCTTGCTTCTACTTACCACATTTAATTT
  TAGGATAACAACNAAGTTCATTCACATTTTCTAAAAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001510
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001510.1, NP_001501.1
• RefSeq Size: 5354 bp
• RefSeq ORF: 3024 bp
• Locus ID: 2895
• UniProt ID: O43424
• Cytogenetics: 4q22.1-q22.2
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Long-term depression, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.