RUNX2 (NM_004348) Human Untagged Clone

CAT#: SC123971

RUNX2 (untagged)-Human runt-related transcription factor 2 (RUNX2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RUNX2
• Synonyms: AML3; CBFA1; CCD; CCD1; OSF-2; OSF2; PEA2aA; PEBP2A1; PEBP2A2; PEBP2aA; PEBP2aA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC123971 sequence for NM_004348 edited (data generated by NextGen Sequencing)
  ATGCGTATTCCCGTAGATCCGAGCACCAGCCGGCGCTTCAGCCCCCCCTCCAGCAGCCTG
  CAGCCCGGCAAAATGAGCGACGTGAGCCCGGTGGTGGCTGCGCAACAGCAGCAGCAACAG
  CAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGGAGGCGGCG
  GCGGCGGCTGCGGCGGCGGCGGCGGCTGCGGCGGCGGCAGCTGCAGTGCCCCGGTTGCGG
  CCGCCCCACGACAACCGCACCATGGTGGAGATCATCGCCGACCACCCGGCCGAACTCGTC
  CGCACCGACAGCCCCAACTTCCTGTGCTCGGTGCTGCCCTCGCACTGGCGCTGCAACAAG
  ACCCTGCCCGTGGCCTTCAAGGTGGTAGCCCTCGGAGAGGTACCAGATGGGACTGTGGTT
  ACTGTCATGGCGGGTAACGATGAAAATTATTCTGCTGAGCTCCGGAATGCCTCTGCTGTT
  ATGAAAAACCAAGTAGCAAGGTTCAACGATCTGAGATTTGTGGGCCGGAGTGGACGAGGC
  AAGAGTTTCACCTTGACCATAACCGTCTTCACAAATCCTCCCCAAGTAGCTACCTATCAC
  AGAGCAATTAAAGTTACAGTAGATGGACCTCGGGAACCCAGAAGGCACAGACAGAAGCTT
  GATGACTCTAAACCTAGTTTGTTCTCTGACCGCCTCAGTGATTTAGGGCGCATTCCTCAT
  CCCAGTATGAGAGTAGGTGTCCCGCCTCAGAACCCACGGCCCTCCCTGAACTCTGCACCA
  AGTCCTTTTAATCCACAAGGACAGAGTCAGATTACAGACCCCAGGCAGGCACAGTCTTCC
  CCGCCGTGGTCCTATGACCAGTCTTACCCCTCCTACCTGAGCCAGATGACGTCCCCGTCC
  ATCCACTCTACCACCCCGCTGTCTTCCACACGGGGCACTGGGCTTCCTGCCATCACCGAT
  GTGCCTAGGCGCATTTCAGATGATGACACTGCCACCTCTGACTTCTGCCTCTGGCCTTCC
  ACTCTCAGTAAGAAGAGCCAGGCAGGTGCTTCAGAACTGGGCCCTTTTTCAGACCCCAGG
  CAGTTCCCAAGCATTTCATCCCTCACTGAGAGCCGCTTCTCCAACCCACGAATGCACTAT
  CCAGCCACCTTTACTTACACCCCGCCAGTCACCTCAGGCATGTCCCTCGGTATGTCCGCC
  ACCACTCACTACCACACCTACCTGCCACCACCCTACCCCGGCTCTTCCCAAAGCCAGAGT
  GGACCCTTCCAGACCAGCAGCACTCCATATCTCTACTATGGCACTTCGTCAGGATCCTAT
  CAGTTTCCCATGGTGCCGGGGGGAGACCGGTCTCCTTCCAGAATGCTTCCGCCATGCACC
  ACCACCTCGAATGGCAGCACGCTATTAAATCCAAATTTGCCTAACCAGAATGATGGTGTT
  GACGCTGATGGAAGCCACAGCAGTTCCCCAACTGTTTTGAATTCTAGTGGCAGAATGGAT
  GAATCTGTTTGGCGACCATATTGA
  
  Clone variation with respect to NM_004348.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004348 unedited
  ACTGCAGTGCACTTNCGAGAGNATATGGCGAACGCGAACTGGGGGCCAGGTCTCGCCTTC
  ACCCCCCCAATTTCCTCCTTGCCCTTTATTTCCACCCTCCTCCCCCTCCCCCGGCGGGTT
  CGCTAACTTGTGGCTGTGGGTGATGCGTATTCCCGTAGATCCGAGCACCAGCCGGCGCTT
  CAGCCCCCCCTCCAGCAGCCTGCAGCCCGGCAAAATGAGCGACGTGAGCCCGGTGGTGGC
  TGCGCAACAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCA
  GCAGCAGCAGCAGGAGGCGGCGGCGGCGGCTGCGGCGGCGGCGGCGGCTGCGGCGGCGGC
  AGCTGCAGTGCCCCGGTTGCGGCCGCCCCACGACAACCGCACCATGGTGGAGATCATCGC
  CGACCACCCGGCCGAACTCGTCCGCACCGACAGCCCCAACTTCCTGTGCTCGGTGCTGCC
  CTCGCACTGGCGCTGCAACAAGACCCTGCCCGTGGCCTTCAAGGTGGTAGCCCTCGGAGA
  GGTACCAGATGGGACTGTGGTTACTGTCATGGCGGGTAACGATGAAAATTATTCTGCTGA
  GCTCCGGAATGCCTCTGCTGTTATGAAAAACCAAGTAGCAAGGTTCAACGATCTGAGATT
  TGTGGGCCGGAGTGGACGAGGCAAGAGTTTCACCTTGACCATAACCGTCTTCACAATCCT
  CCCCAGTAGCTACCTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004348 unedited
  GTATTCTTTGTACCCGCCTGACAGTTACCACAGTCCCATCTGGTAGCTCTCCGAGGGCTA
  CCACCTTGAAGGCCACGGGCAGGGTTTTGTTGCAGCGCCAGTGCGAGGGCAGCACCGAGC
  ACAGGAAGTTGGGGCTGTCGGTGCGGACGAGTTCGGCCGGGTGGTCGGCGATGATCTCCA
  CCATGGTGCGGTTGTCGTGGGGCGGCCGCAACCGGGGCACTGCAGCTGCCGCCGCCGCAG
  CCGCCGCCGCCGCCGCAGCCGCCGCCGCCGCCTCCTGCTGCTGCTGCTGCTGTTGCTGCT
  GCTGCTGCTGCTGTTGCTGCTGCTGCTGTTGCTGCTGCTGTTGCGCAGCCACCACCGGGC
  TCACGTCGCTCATTTTGCCGGGCTGCAGGCTGCTGGAGGGGGGGCTGAAGCGCCGGCTGG
  TGCTCGGATCTACGGGAATACGCATCACAACAGCCACAAGTTAGCGAAGTGGCCGGGGGA
  GGGGGAGGAGGGTGGAAATGAGGGGCAAGGAGGAAATTGGGGGGGTGAANGCNANNACNN
  GNTGCNGAANGNNNGANNNGCCCNAGAGTGAATCGAGATTACAAGAATTTTGGACAGGAT
  TTACTAAATGCGACGCTTCGCTTATAGAACAAGTAGCCTACAACACCATGTCTCGACTGG
  CATATCGGTGACCATTTTTCACGTCCTCTACCGCGGGCCCTGGTGTATTAATCTGAACGA
  GACTGTGTTATGGGAGAGAGGACGACCTCGCCTTGACGTTGTCGAAGACGCGCCGCAGAT
  GCCAATATGAGGTCACTGATGTTGGAATCGTCGAGTAATTGGGACGATTGCAGACAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004348
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004348.3, NP_004339.3
• RefSeq Size: 5720 bp
• RefSeq ORF: 1524 bp
• Locus ID: 860
• Cytogenetics: 6p21.1
• Domains: Runt
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (3) is transcribed from an alternate promoter (P2) and contains a different segment for the 5' UTR and 5' coding region than variant 1. It encodes a protein (isoform c) with a shorter and distinct N-terminus when it is compared to isoform a.