CLCA1 (NM_001285) Human Untagged Clone

CAT#: SC123906

CLCA1 (untagged)-Human chloride channel accessory 1 (CLCA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLCA1
• Synonyms: CACC; CaCC-1; CACC1; CLCRG1; GOB5; hCaCC-1; hCLCA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC123906 sequence for NM_001285 edited (data generated by NextGen Sequencing)
  ATGGGGCCATTTAAGAGTTCTGTGTTCATCTTGATTCTTCACCTTCTAGAAGGGGCCCTG
  AGTAATTCACTCATTCAGCTGAACAACAATGGCTATGAAGGCATTGTCGTTGCAATCGAC
  CCCAATGTGCCAGAAGATGAAACACTCATTCAACAAATAAAGGACATGGTGACCCAGGCA
  TCTCTGTATCTGTTTGAAGCTACAGGAAAGCGATTTTATTTCAAAAATGTTGCCATTTTG
  ATTCCTGAAACATGGAAGACAAAGGCTGACTATGTGAGACCAAAACTTGAGACCTACAAA
  AATGCTGATGTTCTGGTTGCTGAGTCTACTCCTCCAGGTAATGATGAACCCTACACTGAG
  CAGATGGGCAACTGTGGAGAGAAGGGTGAAAGGATCCACCTCACTCCTGATTTCATTGCA
  GGAAAAAAGTTAGCTGAATATGGACCACAAGGTAGGGCATTTGTCCATGAGTGGGCTCAT
  CTACGATGGGGAGTATTTGACGAGTACAATAATGATGAGAAATTCTACTTATCCAATGGA
  AGAATACAAGCAGTAAGATGTTCAGCAGGTATTACTGGTACAAATGTAGTAAAGAAGTGT
  CAGGGAGGCAGCTGTTACACCAAAAGATGCACATTCAATAAAGTTACAGGACTCTATGAA
  AAAGGATGTGAGTTTGTTCTCCAATCCCGCCAGACGGAGAAGGCTTCTATAATGTTTGCA
  CAACATGTTGATTCTATAGTTGAATTCTGTACAGAACAAAACCACAACAAAGAAGCTCCA
  AACAAGCAAAATCAAAAATGCAATCTCCGAAGCACATGGGAAGTGATCCGTGATTCTGAG
  GACTTTAAGAAAACCACTCCTATGACAACACAGCCACCAAATCCCACCTTCTCATTGCTG
  CAGATTGGACAAAGAATTGTGTGTTTAGTCCTTGACAAATCTGGAAGCATGGCGACTGGT
  AACCGCCTCAATCGACTGAATCAAGCAGGCCAGCTTTTCCTGCTGCAGACAGTTGAGCTG
  GGGTCCTGGGTTGGGATGGTGACATTTGACAGTGCTGCCCATGTACAAAGTGAACTCATA
  CAGATAAACAGTGGCAGTGACAGGGACACACTCGCCAAAAGATTACCTGCAGCAGCTTCA
  GGAGGGACGTCCATCTGCAGCGGGCTTCGATCGGCATTTACTGTGATTAGGAAGAAATAT
  CCAACTGATGGATCTGAAATTGTGCTGCTGACGGATGGGGAAGACAACACTATAAGTGGG
  TGCTTTAACGAGGTCAAACAAAGTGGTGCCATCATCCACACAGTCGCTTTGGGGCCCTCT
  GCAGCTCAAGAACTAGAGGAGCTGTCCAAAATGACAGGAGGTTTACAGACATATGCTTCA
  GATCAAGTTCAGAACAATGGCCTCATTGATGCTTTTGGGGCCCTTTCATCAGGAAATGGA
  GCTGTCTCTCAGCGCTCCATCCAGCTTGAGAGTAAGGGATTAACCCTCCAGAACAGCCAG
  TGGATGAATGGCACAGTGATCGTGGACAGCACCGTGGGAAAGGACACTTTGTTTCTTATC
  ACCTGGACAACGCAGCCTCCCCAAATCCTTCTCTGGGATCCCAGTGGACAGAAGCAAGGT
  GGCTTTGTAGTGGACAAAAACACCAAAATGGCCTACCTCCAAATCCCAGGCATTGCTAAG
  GTTGGCACTTGGAAATACAGTCTGCAAGCAAGCTCACAAACCTTGACCCTGACTGTCACG
  TCCCGTGCGTCCAATGCTACCCTGCCTCCAATTACAGTGACTTCCAAAACGAACAAGGAC
  ACCAGCAAATTCCCCAGCCCTCTGGTAGTTTATGCAAATATTCGCCAAGGAGCCTCCCCA
  ATTCTCAGGGCCAGTGTCACAGCCCTGATTGAATCAGTGAATGGAAAAACAGTTACCTTG
  GAACTACTGGATAATGGAGCAGGTGCTGATGCTACTAAGGATGACGGTGTCTACTCAAGG
  TATTTCACAACTTATGACACGAATGGTAGATACAGTGTAAAAGTGCGGGCTCTGGGAGGA
  GTTAACGCAGCCAGACGGAGAGTGATACCCCAGCAGAGTGGAGCACTGTACATACCTGGC
  TGGATTGAGAATGATGAAATACAATGGAATCCACCAAGACCTGAAATTAATAAGGATGAT
  GTTCAACACAAGCAAGTGTGTTTCAGCAGAACATCCTCGGGAGGCTCATTTGTGGCTTCT
  GATGTCCCAAATGCTCCCATACCTGATCTCTTCCCACCTGGCCAAATCACCGACCTGAAG
  GCGGAAATTCACGGGGGCAGTCTCATTAATCTGACTTGGACAGCTCCTGGGGATGATTAT
  GACCATGGAACAGCTCACAAGTATATCATTCGAATAAGTACAAGTATTCTTGATCTCAGA
  GACAAGTTCAATGAATCTCTTCAAGTGAATACTACCGCTCTCATCCCAAAGGAAGCCAAC
  TCTGAGGAAGTCTTTTTGTTTAAACCAGAAAACATTACTTTTGAAAATGGCACAGATCTT
  TTCATTGCTATTTAGGCTGTTGATAAGGTCGATCTGAAATCAGAAATATCCAACATTGCA
  CGAGTATCTTTGTTTATTCCTCCACAGACTCCGCCAGAGACACCTAGTCCTGATGAAACG
  TCTGCTCCTTGTCCTAATATTCATATCAACAGCACCATTCCTGGCATTCACATTTTAAAA
  ATTATGTGGAAGTGGATAGGAGAACTGCAGCTGTCAATAGCCTAG
  
  Clone variation with respect to NM_001285.3
  645 a=>t;1070 a=>g;1571 t=>c;2436 t=>c;2533 c=>t
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001285 unedited
  TCCGGGCCAGGATAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTGGGGGTTTTTTTTTTTT
  TTTTTTTTTTCCCCAGTTGTTTAGCATTTTATTTTATCGCCCCCTACAGGAAGTCTAAAA
  TACATTTAGTATATCGCCCCCTACAGGAATACATTTAGTATAAATGTACTATATACATTT
  AGTATATCGCCCCCTACAGGAAGTCTAAAATACATTTTAGAAAATTTTATAATCAAAAAA
  AAGGATGAATGATTTATTTTATTTATCTGACAAAAATTCAGCCCTAGGCTATTGACAGCT
  GCAGTTCTCCTATCCACTTCCACATAATTTTTAAAATGTGAATGCCAGGAATGGTGCTGT
  TGATATGAATATTAGGACAAGGAGCAGACGTTTCATCAGGACTAGGTGTCTCTGGCGGAG
  TCTGTGGAGGAATAAACAAAGATACTCGTGCAATGTTGGATATTTCTGATTTCAGATCGA
  CCTTATCAACAGCCTAAATAGCAATGAAAAGATCTGTGCCATTTTCAAAAGTAATGTTTT
  CTGGTTTAAACAAAAAGACTTCCTCAGAGTTGGCTTCCTTTGGGATGAGAGCGGTAGTAT
  TCACTTGAAGAGATTCATTGAACTTGTCTCTGAGATCAAGAATACTTGTACTTATTCGAA
  TGATATACTTGTGAGCTGTTCCATGGCATAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001285
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285.1, NP_001276.1
• RefSeq Size: 3311 bp
• RefSeq ORF: 3123 bp
• Locus ID: 1179
• UniProt ID: A8K7I4
• Cytogenetics: 1p22.3
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Olfactory transduction
• Gene Summary: This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq, Jul 2008]