NGF (NM_002506) Human Untagged Clone

CAT#: SC123827

NGF (untagged)-Human nerve growth factor (beta polypeptide) (NGF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NGF
• Synonyms: Beta-NGF; HSAN5; NGFB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002506 edited
  CCACGCGTCCGCGGACGCGTGGGCGCTGGGAGCCGGAGGGGAGCGCAGCGAGTTTTGGCC
  AGTGGTCGTGCAGTCCAAGGGGCTGGATGGCATGCTGGACCCAAGCTCAGCTCAGCGTCC
  GGACCCAATAACAGTTTTACCAAGGGAGCAGCTTTCTATCCTGGCCACACTGAGGTGCAT
  AGCGTAATGTCCATGTTGTTCTACACTCTGATCACAGCTTTTCTGATCGGCATACAGGCG
  GAACCACACTCAGAGAGCAATGTCCCTGCAGGACACACCATCCCCCAAGTCCACTGGACT
  AAACTTCAGCATTCCCTTGACACTGCCCTTCGCAGAGCCCGCAGCGCCCCGGCAGCGGCG
  ATAGCTGCACGCGTGGCGGGGCAGACCCGCAACATTACTGTGGACCCCAGGCTGTTTAAA
  AAGCGGCGACTCCGTTCACCCCGTGTGCTGTTTAGCACCCAGCCTCCCCGTGAAGCTGCA
  GACACTCAGGATCTGGACTTCGAGGTCGGTGGTGCTGCCCCCTTCAACAGGACTCACAGG
  AGCAAGCGGTCATCATCCCATCCCATCTTCCACAGGGGCGAATTCTCGGTGTGTGACAGT
  GTCAGCGTGTGGGTTGGGGATAAGACCACCGCCACAGACATCAAGGGCAAGGAGGTGATG
  GTGTTGGGAGAGGTGAACATTAACAACAGTGTATTCAAACAGTACTTTTTTGAGACCAAG
  TGCCGGGACCCAAATCCCGTTGACAGCGGGTGCCGGGGCATTGACTCAAAGCACTGGAAC
  TCATATTGTACCACGACTCACACCTTTGTCAAGGCGCTGACCATGGATGGCAAGCAGGCT
  GCCTGGCGGTTTATCCGGATAGATACGGCCTGTGTGTGTGTGCTCAGCAGGAAGGCTGTG
  AGAAGAGCCTGACCTGCCGACACGCTCCCTCCCCCTGCCCCTTCTACACTCTCCTGGGCC
  CCTCCCTACCTCAACCTGTAAATTATTTTAAATTATAAGGACTGCATGGTAATTTATAGT
  TTATACAGTTTTAAAGAATCATTATTTATTAAATTTTTGGAAGCAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002506 unedited
  GGGGAGTTCACATTTGTATACGACTCATATAGGCGGCCGACGATTCATATCTGGTACCGG
  TCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCGGACGCGTGGGCGCTGGGAGCC
  GGAGGGGAGCGCAGCGAGTTTTGGCCAGTGGTCGTGCAGTCCAAGGGGCTGGATGGCATG
  CTGGACCCAAGCTCAGCTCAGCGTCCGGACCCAATAACAGTTTTACCAAGGGAGCAGCTT
  TCTATCCTGGCCACACTGAGGTGCATAGCGTAATGTCCATGTTGTTCTACACTCTGATCA
  CAGCTTTTCTGATCGGCATACAGGCGGAACCACACTCAGAGAGCAATGTCCCTGCAGGAC
  ACACCATCCCCCAAGTCCACTGGACTAAACTTCAGCATTCCCTTGACACTGCCCTTCGCA
  GAGCCCGCAGCGCCCCGGCAGCGGCGATAGCTGCACGCGTGGCGGGGCAGACCCGCAACA
  TTACTGTGGACCCCAGGCTGTTTAAAAAGCGGCGACTCCGTTCACCCCGTGTGCTGTTTA
  GCACCCAGCCTCCCCGTGAAGCTGCAGACACTCAGGATCTGGACTTCGAGGTCGGTGGTG
  CTGCCCCCTTCAACAGGACTCACAGGAGCAAGCGGTCATCATCCCATCCCATCTTCCACA
  GGGGCGAATTCTCGGTGTGTGACAGTGTCAGCGTGTGGGTTGGGGATAAGACCACCGCCA
  CAGACATCAAGGGCAAGGAGGTGATGGTGTTGGGAGAGGTGAACATTAACAACAGTGTAT
  TCAAACAGTACTTTTTTGAGACCAAGTGCCGGGACCCAAATCCCGTTGACAGCGGGTGCC
  GGNGCATTGACTCANACACTGGNACTCATATTGTACCACGACTCACACCTTTNGTCAGGC
  GCTGACCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002506 unedited
  CATAGTCATGGCATGGCACTTGCCAGGGCCAGGANAGCACTGGGNGAGGGTCACAGGGAT
  GCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCCCTTTTTTTTTTTTTTT
  GCTTCCAAAAATTTAATAAATAATGATTCTTTAAAACTGTATAAACTATAAATTACCATG
  CAGTCCTTATAATTTAAAATAATTTACAGGTTGAGGTAGGGAGGGGCCCAGGAGAGTGTA
  AAAGGGGCAGGGGGAGGGAGCGTGTCGGCAGGTCAGGCTCTTCTCACAGCCTTCCTGCTG
  AGCACACACACACAGGCCGTATCTATCCGGATAAACCGCCAGGCAGCCTGCTTGCCATCC
  ATGGTCAGCGCCTTGACAAAGGTGTGAGTCGTGGTACAATATGAGTTCCAGTGCTTTGAG
  TCAATGCCCCGGCACCCGCTGTCAACGGGATTTGGGTCCCGGCACTTGGTCTCAAAAAAG
  TACTGTTTGAATACACTGTTGTTAATGTTCACCTCTCCCAACACCATCACCTCCTTGCCC
  TTGATGTCTGTGGCGGTGGTCTTATCCCCAACCCACACGCTGACACTGTCACACACCGAG
  AATTCNCCCCTGTGGAAGATGGGATGGGATGATGACCGCTTGCTCCTGTGAGTCCTGTTG
  AAGGGGGCAGCACCACCGACCTCGAAGTCCAGATCCTGAGTGTCTGCAGCTTCACGGGGA
  GGCTGGGTGCTAAACAGCACACGGTGTGAACGGAGTCGCCGCTTTTTAAACAGCCTGGGG
  TCCACAGTAATGGTTGCGGGTCTGCCCCGCCACGCGTGCAACTTTCGCCGCTGCCGGGGC
  GCTCGGGCTCTTGAAAGGGCAGTGTCAAGGTATGCTTAAATTTAATCCGTGGACTGGGGG
  AGTGC
  
• Restriction Sites: Please inquire
• ACCN: NM_002506
• Insert Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002506.2, NP_002497.2
• RefSeq Size: 1052 bp
• RefSeq ORF: 726 bp
• Locus ID: 4803
• UniProt ID: P01138
• Cytogenetics: 1p13.2
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]