UBE2B (BC001694) Human Untagged Clone

CAT#: SC123788

(untagged)-Homo sapiens, clone MGC:2299 IMAGE:2967519, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2B
• Synonyms: E2-17kDa; HHR6B; HR6B; RAD6B; UBC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC001694 edited
  GGCACGAGGGGAGTCGGGAGAGGGGTTCGCCCCAACACGGCTCCTCGGAGAAGACGGCGG
  GGCCGTGAAACAAAGCTGACTGGTGCTGCCGGAGAGGAACAAGCTCCCGCAGGAGCTCAC
  TTTGAGGGGACAAGAAGGGGGCGCCGCAGGGTGCAGGGGCGGGCGGATAATGTCTGATCG
  CCTGGGCGAGACGCCGGGCAGCTGGCCGAAATGGGCAGCAGGGAACCCTAGACCAGTCGA
  TCGACACCGTTTTACTTTTTGTGGGGAGGGGAAGCAGTATCAAAATTTCTTCGGGCGCCA
  GAACGAGCGGCAAAGCTTATGGGAGTAGGGCCGAGGCTAGGACCCGGCTTCCGCCAGTCC
  CGCTGGGCCCACAGCCGGCCCTCTGAGGGTCACAAAGGGGAAGGTGTCTGGGATCCACGC
  CCTGCAGCCCCGCAGCTTTGAGGGGCCGTCATCTAGGCGAAGGTGAAGGCCCTTACCGCT
  TGAAATCCCGCATGAGCCTCCTCCGGGCCGGGGTCGACATGCTCCGCAGCTGCCCCGCGG
  TCAGTCTGAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC001694 unedited
  GCACGAGGGGAGTCGGGAGAGGGGTTCGCCCCAACACGGCTCCTCGGAGAAGACGGCGGG
  GCCGTGAAACAAAGCTGACTGGTGCTGCCGGAGAGGAACAAGCTCCCGCAGGAGCTCACT
  TTGAGGGGACAAGAAGGGGGCGCCGCAGGGTGCAGGGGCGGGCGGATAATGTCTGATCGC
  CTGGGCGAGACGCCGGGCAGCTGGCCGAAATGGGCAGCAGGGAACCCTAGACCAGTCGAT
  CGACACCGTTTTACTTTTTGTGGGGAGGGGAAGCAGTATCAAAATTTCTTCGGGCGCCAG
  AACGAGCGGCAAAGCTTATGGGAGTAGGGCCGAGGCTAGGACCCGGCTTCCGCCAGTCCC
  GCTGGGCCCACAGCCGGCCCTCTGAGGGTCACAAAGGGGAAGGTGTCTGGGATCCACGCC
  CTGCAGCCCCGCAGCTTTGAGGGGCCGTCATCTAGGCGAAGGTGAAGGCCCTTACCGCTT
  GAAATCCCGCATGAGCCTCCTCCGGGCCGGGGTCGACATGCTCCGCAGCTGCCCCGCGGT
  CAGTCTGAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAGTT
  
• Restriction Sites: Please inquire
• ACCN: BC001694
• Insert Size: 574 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC001694.1, AAH01694.1
• RefSeq Size: 574 bp
• Locus ID: 7320
• Cytogenetics: 5q31.1
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]