ERCC8 (BC009793) Human Untagged Clone

CAT#: SC123696

ERCC8 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 8, transcript variant 3 (cDNA clone MGC:13580 IMAGE:3683434), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERCC8
• Synonyms: CKN1; Cockayne syndrome WD-repeat protein CSA; CSA; DNA excision repair protein ERCC-8; excision repair cross-complementing rodent repair deficiency, complementation group 8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC009793 edited
  GGGGGTCATGGCGACGTCCAGTGCTCCAGCCGGTGTGAGGACACGATATGCTGGGGTTTT
  TGTCCGCACGCCAAACGGGTTTGGAGGACCCTCTTCGCCTTCGGAGAGCAGAGTCAACAC
  GGAGAGTTTTGGGACTGGAATTAAATAAAGACAGAGATGTTGAAAGAATCCACGGCGGTG
  GAATTAACACCCTTGACATTGAACCTGTTGAAGGGAGATACATGTTATCAGGTGGTTCAG
  ATGGTGTGATTGTACTTTATGACCTTGAGAACTCCAGCAGACAATCTTATTACACATGTA
  AAGCAGTGTGTTCCATTGGCAGAGATCATCCTGATGTTCACAGATACAGTGTGGAGACTG
  TACAGTGGTATCCTCATGACACTGGCATGTTCACATCAAGCTCATTTGATAAAACTCTGA
  AAGTATGGGATACAAATACATTACAAACTGCAGATGTATTTAATTTTGAGGAAACAGTTT
  ACAGTCATCATATGTCTCCAGTCTCCACCAAGCACTGTTTGGTAGCAGTTGGTACTAGAG
  GACCCAAAGTACAACTTTGTGACTTGAAGTCTGGATCCTGTTCTCACATTCTACAGGGTA
  TTTTTATTTTATTTCAAACGGCAACTACTTTGAGTAAACGATTCAATAAAAAGAAACGTT
  ACTAACAGTGTATTCTTTGTAAGTGACATGACTAATGTACTTTGTGCTGGTTGTTGAGAC
  TCAGCAGGGAAATAAAGATCCTTCTGTGCATTATTCTTATAAAACTGAACCATTGAAAAC
  ACATTTATATGAACAATGATCATTGGGGAAGCTCAAACAATATGAAAAATAGTTCTAACT
  CAAAACCTTGTGTTATTTCTACATTAAAAAAACTAATGCAACTCAGAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC009793 unedited
  ATAGGCGGCCGCGNAATTCGCCATTACGGCCGGGGGTCATGGCGACGTCCAGTGCTCCAG
  CCGGTGTGAGGACACGATATGCTGGGGTTTTTGTCCGCACGCCAAACGGGTTTGGAGGAC
  CCTCTTCGCCTTCGGAGAGCAGAGTCAACACGGAGAGTTTTGGGACTGGAATTAAATAAA
  GACAGAGATGTTGAAAGAATCCACGGCGGTGGAATTAACACCCTTGACATTGAACCTGTT
  GAAGGGAGATACATGTTATCAGGTGGTTCAGATGGTGTGATTGTACTTTATGACCTTGAG
  AACTCCAGCAGACAATCTTATTACACATGTAAAGCAGTGTGTTCCATTGGCAGAGATCAT
  CCTGATGTTCACAGATACAGTGTGGAGACTGTACAGTGGTATCCTCATGACACTGGCATG
  TTCACATCAAGCTCATTTGATAAAACTCTGAAAGTATGGGATACAAATACATTACAAACT
  GCAGATGTATTTAATTTTGAGGAAACAGTTTACAGTCATCATATGTCTCCAGTCTCCACC
  AAGCACTGTTTGGTAGCAGTTGGTACTAGAGGACCCAAAGTACAACTTTGTGACTTGAAG
  TCTGGATCCTGTTCTCACATTCTACAGGGTATTTTTATTTTATTTCAAACGGCAACTACT
  TTGAGTAAACGATTCAATAAAAAGAAACGTTACTAACAGTGTATTCTTTGTAAGTGACAT
  GACTAATGTACTTTGTGCTGGTTGTTGAGACTCAGCAGGGAAATAAAGATCCTTCTGTGC
  ATTATTTCTTATAAACTGAACCCTTGAAAACACATTTATATGAACAATGATCATTGG
  
• Restriction Sites: Please inquire
• ACCN: BC009793
• Insert Size: 916 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009793.1, AAH09793.1
• RefSeq Size: 916 bp
• Locus ID: 1161
• Cytogenetics: 5q12.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]