TFPI (BC015514) Human Untagged Clone

CAT#: SC123622

TFPI (untagged)-Human tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) (cDNA clone MGC:9251 IMAGE:3902987), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TFPI
• Synonyms: EPI; LACI; TFI; TFPI1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC015514 edited
  AACAGACAGCAGCGACTTTAGGCTGGATAATAGTCAAATTCTTACCTCGCTCTTTCACTG
  CTAGTAAGATCAGATTGCGTTCTTTCAGTTACTCTTCAATCGCCAGTTTCTTGATCTGCT
  TCTAAAAGAAGAAGTAGAGAAGATAAATCCTGTCTTCAATACCTGGAAGGAAAAACAAAA
  TAACCTCAACTCCGTTTTGAAAAAAACATTCCAAGAACTTTCATCAGAGATTTTACTTAG
  ATGATTTACACAATGAAGAAAGTACATGCACTTTGGGCTTCTGTATGCCTGCTGCTTAAT
  CTTGCCCCTGCCCCTCTTAATGCTGATTCTGAGGAAGATGAAGAACACACAATTATCACA
  GATACGGAGTTGCCACCACTGAAACTTATGCATTCATTTTGTGCATTCAAGGCGGATGAT
  GGCCCATGTAAAGCAATCATGAAAAGATTTTTCTTCAATATTTTCACTCGACAGTGCGAA
  GAATTTATATATGGGGGATGTGAAGGAAATCAGAATCGATTTGAAAGTCTGGAAGAGTGC
  AAAAAAATGTGTACAAGAGATAATGCAAACAGGATTATAAAGACAACATTGCAACAAGAA
  AAGCCAGATTTCTGCTTTTTGGAAGAAGATCCTGGAATATGTCGAGGTTATATTACCAGG
  TATTTTTATAACAATCAGACAAAACAGTGTGAACGTTTCAAGTATGGTGGATGCCTGGGC
  AATATGAACAATTTTGAGACACTGGAAGAATGCAAGAACATTTGTGAAGATGGTCCGAAT
  GGTTTCCAGGTGGATAATTATGGAACCCAGCTCAATGCTGTGAATAACTCCCTGACTCCG
  CAATCAACCAAGGTTCCCAGCCTTTTTGTTACAAAAGAAGGAACAAATGATGGTTGGAAG
  AATGCGGCTCATATTTACCAAGTCTTTCTGAACGCCTTCTGCATTCATGCATCCATGTTC
  TTTCTAGGATTGGATAGCATTTCATGCCTATGTTAATATTTGTGCTTTTGGCATTTCCTT
  AATATTTATATGTATACGTGATGCCTTTGATAGCATACTGCTAATAAAGTTTTAATATTT
  ACATGCAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC015514 unedited
  GTTCAGATTTGTATACGACTCACTATAGGCGGCCGCGATTCCCGGGNAATCGTCGACCCA
  CGCGTCCGAACANACAGCAGCGACTTTAGGCTGGATAATAGTCAAATTCTTACCTCGCTC
  TTTCACTGCTAGTAAGATCAGATTGCGTTCTTTCAGTTACTCTTCAATCGCCAGTTTCTT
  GATCTGCTTCTAAAAGAAGAAGTAGAGAAGATAAATCCTGTCTTCAATACCTGGAAGGAA
  AAACAAAATAACCTCAACTCCGTTTTGAAAAAAACATTCCAAGAACTTTCATCAGAGATT
  TTACTTAGATGATTTACACAATGAAGAAAGTACATGCACTTTGGGCTTCTGTATGCCTGC
  TGCTTAATCTTGCCCCTGCCCCTCTTAATGCTGATTCTGAGGAAGATGAAGAACACACAA
  TTATCACAGATACGGAGTTGCCACCACTGAAACTTATGCATTCATTTTGTGCATTCAAGG
  CGGATGATGGCCCATGTAAAGCAATCATGAAAAGATTTTTCTTCAATATTTTCACTCGAC
  AGTGCGAAGAATTTATATATGGGGGATGTGAAGGAAATCAGAATCGATTTGAAAGTCTGG
  AAGAGTGCAAAAAAATGTGTACAAGAGATAATGCAAACAGGATTATAAAGACAACATTGC
  AACAAGAAAAGCCAGATTTCTGCTTTTTGGAAGAAGATCCTGGAATATGTCGAGGTTATA
  TTACCAGGTATTTTTATAACAATCAGACAAAACAGTGTGAACGTTTNCAAGTATGGTGGA
  TGCCTGGGCAATATGAACAATTTTGAGACACTGGAAGAATGCAAGAACATTTGTGAAGAT
  GGTCCGAATGGTTTCCAGGTGGATAATTATGGAACCCAGCTCAATGCTGTGAATAACTCC
  CTGACTCCGCATCAACCAGGTTCCNAGNCTTTTTGTTACAAAAGAG
  
• Restriction Sites: Please inquire
• ACCN: BC015514
• Insert Size: 1108 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015514.1, AAH15514.1
• RefSeq Size: 1108 bp
• Locus ID: 7035
• Cytogenetics: 2q32.1
• Protein Families: Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016]