PPP2R1B (NM_181699) Human Untagged Clone

CAT#: SC123371

PPP2R1B (untagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R1B
• Synonyms: PP2A-Abeta; PR65B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_181699 edited
  AGGAGCAGGAGGAGAAAGAACATGGCGGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCA
  GCGGGTGGAGATGGAGATGATTCGCTATACCCGATCGCGGTTTTAATCGACGAGCTCCGC
  AATGAAGACGTGCAGCTCCGACTCAACAGTATTAAGAAGTTATCAACAATTGCCCTAGCA
  CTTGGAGTAGAAAGGACCCGAAGTGAATTGTTGCCATTTCTTACAGATACAATTTATGAT
  GAAGATGAGGTACTATTAGCTCTTGCTGAGCAGCTGGGAAATTTCACTGGCCTAGTGGGA
  GGTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAATCTGGCAACTGTGGAAGAG
  ACTGTTGTTCGTGACAAGGCTGTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCT
  GTTGCTCTGGAAGCTTATTTTGTACCTCTGGTGAAACGCTTAGCAAGTGGGGATTGGTTC
  ACCTCTCGCACATCTGCATGTGGTTTGTTCAGCGTTTGCTATCCCAGGGCATCAAATGCT
  GTTAAAGCAGAAATCAGACAGCAATTCCGTTCCTTGTGCTCAGATGACACACCAATGGTA
  CGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTAGACAGTGTG
  AAAAGTGAAATTGTTCCACTGTTCACTAGTCTAGCTTCAGATGAACAGGATTCAGTGCGC
  CTCCTTGCTGTGGAAGCTTGTGTCAGTATTGCCCAGTTATTGTCTCAGGATGACCTTGAG
  ACTTTGGTGATGCCTACACTTCGACAAGCAGCAGAAGATAAATCTTGGCGCGTTCGCTAT
  ATGGTGGCTGACAGATTTTCAGAGCTCCAGAAAGCCATGGGTCCTAAAATCACCCTAAAT
  GACCTCATCCCCGCCTTTCAGAACCTACTTAAAGACTGTGAAGCTGAAGTCCGGGCTGCT
  GCTGCCCACAAAGTAAAAGAACTTGGTGAGAACTTGCCCATTGAAGATAGAGAGACCATA
  ATTATGAATCAAATTCTGCCTTATATAAAGGAATTAGTATCCGATACCAATCAACATGTC
  AAATCGGCTCTAGCTTCTGTAATTATGGGATTGTCTACTATTTTGGGCAAAGAAAATACC
  ATTGAACATCTTCTACCTCTTTTCTTAGCTCAGTTAAAGGATGAGTGTCCTGACGTTCGT
  TTGAATATCATCTCCAATTTGGATTGTGTAAATGAAGTGATTGGAATCCGTCAGCTCTCT
  CAGTCTCTCCTTCCTGCCATAGTGGAGCTGGCAGAAGATGCCAAATGGAGGGTCCGCCTG
  GCCATCATTGAGTATATGCCGCTGCTGGCAGGCCAGCTGGGTGTGGAATTCTTTGATGAA
  AAGCTGAATTCTTTATGTATGGCTTGGCTCGTGGACCATGTATACGCCATCCGAGAAGCT
  GCCACCAACAACCTCATGAAACTAGTTCAGAAGTTTGGTACAGAGTGGGCCCAAAATACT
  ATTGTTCCCAAAGTGTTAGTAATGGCAAATGATCCTAATTACTTGCATAGAATGACCACT
  TTATTCTGCATTAATGCACTGTCTGAGGCCTGTGGTCAGGAAATAACTACTAAGCAAATG
  CTGCCCATCGTATTAAAAATGGCAGGAGACCAAGTAGCAAATGTTCGCTTCAATGTGGCC
  AAATCTCTACAAAAGATTGGACCAATTCTAGATACCAATGCTTTACAGGGAGAAGTGAAG
  CCAGTACTACAGAAGTTAGGTCAAGATGAAGACATGGATGTCAAATACTTTGCACAGGAA
  GCTATAAGTGTGGTGGCACAAAGGCTGAGGAAGCTAGAATTTCCTGTGAAGGACAGTGGA
  GAACCCAGTGTCCCTCGGGCTGACAAGAACCACTTCCCAAGACCCACAGTGCCTGGAGAG
  GACATGGGGAAGGGACCAGTGTATCAGTTGCGTGGAGATACTAGAGACACACTTGCCCAG
  CTGGGAATTGCAGAGCTAGTGCATTTCTCCCAAAGCACAGACTAATACCCCAGATTTAAA
  AAAAGAACATAAAAAATAAAGTACCACACATATTACAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181699 unedited
  GGCCGAATACACTTCCTATAGCATACATTATACAATANTTATGGTAGTCAGGCCAAGGGG
  GGCGCGCGGCGGGGATTCATAAAAAGCGGAGGAGCAGGAGGAGAAAGAACATGGCGGGCG
  CATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTGGAGATGGAGATGATTCGCTATACC
  CGATCGCGGTTTTTATTTACGAGCTCCGCAATGAAGACGTGGGGGGGCGACTCAACAGTA
  TTAAGAAGTTATCAACAATTGCCCTAGCACTTGGAGTAGAAAGGACCCGAAGTGAATTGT
  TGCCATTTCTTACAGATACAATTTATGATGAAGATGAGGTACTATTAGCTCTTGCTGAGC
  AGCTGGGAAATTTCACTGGCCTAGTGGGAGGTCCTGACTTTGCCCACTGTCTGCTGCCTC
  CTTTGGAAAATCTGGCAACTGTGGAAGAGACTGTTGTTCGTGACAAGGCTGTGGAGTCCC
  TGAGACAGATCTCCCAGGAGCATACTCCTGTTGCTCTGGAAGCTTATTTTGTACCTCTGG
  TGAAACGCTTAGCAAGTGGGGATTGGTTCACCTCTCGCACATCTGCGTGTGGTTTGGTCA
  GCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAAGCAGAAATCAGACAGCAATTCCGTT
  CCTTGTGCTCAGATGACACACCAATGGTACGACGTGCTGCTGCTTCCAAATTGGGTGAAT
  TGCAAAAGTGTTGGAATTAGACAGTGTGAAAAGGGAATTGTTGCCCTGTTCGCTAGTCTA
  GCTCCAGATGAACGGGATTCAGTGCGCCTCCTTGGTGGGAGAACTTGGGTCATTATTGCC
  CAATTTTGGCTCAGGGGAACCTTGAAACTTGGGGGAGCGCACCTTCCAACAGCGGGN
  
• Restriction Sites: Please inquire
• ACCN: NM_181699
• Insert Size: 2097 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181699.1, NP_859050.1
• RefSeq Size: 2139 bp
• RefSeq ORF: 2004 bp
• Locus ID: 5519
• UniProt ID: P30154
• Cytogenetics: 11q23.1
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• Gene Summary: This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (2) differs in the 3' UTR and the 3' coding region, compared to variant 1. The resulting isoform (b) contains a longer and distinct C-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.