IL5RA (NM_175728) Human Untagged Clone

CAT#: SC123343

IL5RA (untagged)-Human interleukin 5 receptor, alpha (IL5RA), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL5RA
• Synonyms: CD125; CDw125; HSIL5R3; IL5R
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_175728 edited
  ACGGTTGCTCTGTACAAATCCTCCTCCATATTTGCTTAGAGAAAACGTGTTGCCATCCCA
  TCATGAAGGAAGCTGCCTGAGAGTTTTTAACCATTACAGCCGTGATGATGAAAGAGTGAA
  GAACCGCCTCTAAGTTAAAAAGTGCACCCAGAGATAAGGTTCGTTCTCAATGCTCTGCCG
  CTGCTTCTCATCGCATGGCCACCGCATTTCTCAGGCCAGGCACATTGAGCATTGGTCCTG
  TGCCTGACGCTATGCTAGATGCTGGGGTTGCAGCCACGAGCATAGACACGACAGACACGG
  TCCTCGCCATCTTCTGTTGAGTACTGGTCGGAACAAGAGGATCGTCTGTAGACAGGATAT
  GATCATCGTGGCGCATGTATTACTCATCCTTTTGGGGGCCACTGAGATACTGCAAGCTGA
  CTTACTTCCTGATGAAAAGATTTCACTTCTCCCGCCTGTCAATTTCACCATTAAAGTTAC
  TGGTTTGGCTCAAGTTCTTTTACAATGGAAACCAAATCCTGATCAAGAGCAAAGGAATGT
  TAATCTAGAATATCAAGTGAAAATAAACGCTCCAAAAGAAGATGACTATGAAACCAGAAT
  CACTGAAAGCAAATGTGTAACCATCCTCCACAAGGGCTTTTCAGCAAGTGTGCGGACCAT
  CCTGCAGAACGACCACTCACTACTGGCCAGCAGCTGGGCTTCTGCTGAACTTCATGCCCC
  ACCAGGGTCTCCTGGAACCTCAATTGTGAATTTAACTTGCACCACAAACACTACAGAAGA
  CAATTATTCACGTTTAAGGTCATACCAAGTTTCCCTTCACTGCACCTGGCTTGTTGGCAC
  AGATGCCCCTGAGGACACGCAGTATTTTCTCTACTATAGGTATGGCTCTTGGACTGAAGA
  ATGCCAAGAATACAGCAAAGACACACTGGGGAGAAATATCGCATGCTGGTTTCCCAGGAC
  TTTTATCCTCAGCAAAGGGCGTGACTGGCTTGCGGTGCTTGTTGACGGCTCCAGCAAGCA
  CTCTGCTATCAGGCCCTTTGATCAGCTGTTTGCCCTTCACGCCATTGATCAAATAAATCC
  TCCACTGAATGTCACAGCAGAGATTGAAGGAACTCGTCTCTCTATCCAATGGGAGAAACC
  AGTGTCTGCTTTTCCAATCCATTGCTTTGATTATGAAGTAAAAATACACAATACAAGGAA
  TGGATATTTGCAGATAGAAAAATTGATGACCAATGCATTCATCTCAATAATTGATGATCT
  TTCTAAGTACGATGTTCAAGTGAGAGCAGCAGTGAGCTCCATGTGCAGAGAGGCAGGGCT
  CTGGAGTGAGTGGAGCCAACCTATTTATGTGGGGTTCTCAAGATAAAGGAGATAACATCC
  AGCTTTCCTGCCCCACACCGTATCTGAAATAAAAACAACAGCAGGAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175728 unedited
  CCTGTTAGCATTTGTAAACGACTTATATAGGCGGCCGCATAACTTCGTATAGCATACATT
  ATATGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGACGGTT
  GCTCTGTACAAATCCTCCTCCATATTTGCTTAGAGAAAACGTGTTGCCATCCCATCATGA
  AGGAAGCTGCCTGAGAGTTTTTAACCATTACAGCCGTGATGATGAAAGAGTGAAGAACCG
  CCTCTGAGTTAAAAAGTGCACCCAGAGATAAGGTTCGTTCTCAATGCTCTGCCGCTGCTT
  CTCATCGCATGGCCACCGCATTTCTCAGGCCAGGCACATTGAGCATTGGTCCTGTGCCTG
  ACGCTATGCTAGATGCTGGGGTTGCAGCCACGAGCATAGACACGACAGACACGGTCCTCG
  CCATCTTCTGTTGAGTACTGGTCGGAACAAGAGGATCGTCTGTAGACAGGATATGATCAT
  CGTGGCGCATGTATTACTCATCCTTTTGGGGGCCACTGAGATACTGCAAGCTGACTTACT
  TCCTGATGAAAAGATTTCACTTCTCCCGCCTGTCAATTTCACCATTAAAGTTACTGGTTT
  GGCTCAAGTTCTTTTACAATGGAAACCAAATCCTGATCAAGAGCAAAGGAATGTTAATCT
  AGAATATCAAGTGAAAATAAACGCTCCAAAAGAAGATGACTATGANACCAGAATCACTGA
  NAGCAAATGTGTAACCATCCTCCACAAGGGCTTTTCAGCAAGTGTGCGGACCATCCTGCA
  GAACGACCACTCACTACTGGCCAGCAGCTGGGCTTCTGCTGAACTTCATGCCCCACCAGG
  GTCTCCTGGAACCTCATTGTGAATTTAACTGCACCACAACACTACAGAAGACATTATTCC
  GTTTAAGTCATACCN
  
• Restriction Sites: Please inquire
• ACCN: NM_175728
• Insert Size: 1445 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175728.1, NP_783855.1
• RefSeq Size: 1626 bp
• RefSeq ORF: 1008 bp
• Locus ID: 3568
• UniProt ID: Q01344
• Cytogenetics: 3p26.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
• Gene Summary: The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]
  Transcript Variant: This variant (6) lacks an internal segment within the 5' UTR, and differs in the 3' end region, when compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus, as compared to isoform 1.