RNF183 (NM_145051) Human Untagged Clone

CAT#: SC123208

RNF183 (untagged)-Human ring finger protein 183 (RNF183)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF183
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_145051 edited
  CGATTCAGGGGAGGGAGCAACTGGAGCCTCAGGCCCTCCAGAGTAGTCTGCCTGACCACC
  CTGGAGCCCACAGAAGCCCAGGACGTCTCCCGCGAGGCCTCCCCGTGTGTGGCTGAGGAT
  GGCTGAGCAGCAGGGCCGGGAGCTTGAGGCTGAGTGCCCCGTCTGCTGGAACCCCTTCAA
  CAACACGTTCCATACCCCCAAAATGCTGGATTGCTGCCACTCCTTCTGCGTGGAATGTCT
  GGCCCACCTCAGCCTTGTGACTCCAGCCCGGCGCCGCCTGCTGTGCCCACTCTGTCGCCA
  GCCCACAGTGCTGGCCTCAGGGCAGCCTGTCACTGACTTGCCCACGGACACTGCCATGCT
  CACCCTGCTCCGCCTGGAGCCCCACCATGTCATCCTGGAAGGCCATCAGCTGTGCCTCAA
  GGACCAGCCCAAGAGCCGCTACTTCCTGCGCCAGCCTCGAGTCTACACGCTGGACCTTGG
  CCCCCAGCCTGGGGGCCAGACTGGGCCGCCCCCAGACACGGCCTCTGCCACCGTGTCTAC
  GCCCATCCTCATCCCCAGCCACCACTCTTTGAGGGAGTGTTTCCGCAACCCTCAGTTCCG
  CATCTTTGCCTACCTGATGGCCGTCATCCTCAGTGTCACTCTGTTGCTCATATTCTCCAT
  CTTTTGGACCAAGCAGTTCCTTTGGGGTGTGGGGTGAGTGCTGTTCCCAGACAAGAAACC
  AAACCTTTTTCGGTTGCTGCTGGGTATGGTGACTACGGAGCCTCATTTGGTATTGTCTTC
  CTTTGTAGTGTTGTTTATTTTACAATCCAGGGATTGTTCAGGCCATGTGTTTGCTTCTGG
  GAACAATTTAAAAAAAAAACAAAAAAACGAAAAGCTTGAAGGACTGGGAGATGTGGAGCG
  ACCTCCGGGTGTGAGTGTGGCGTCATGGAAGGGCAGAGAAGCGGTTCTGACCACAGAGCT
  CCACAGCAAGTTGTGCCAAAGGGCTGCACAGTGGTATCCAGGAACCTGACTAGCCCAAAT
  AGCAAGTTGCATTTCTCACTGGAGCTGCTTCAAAATCAGTGCATATTTTTTTGAGTTGCT
  CTTTTACTATGGGTTGCTAAAAAAAAAAAAAAAAAAATTGGGAAGTGAGCTTCAATTCTG
  TGGGTAAATGTGTGTTTGTTTCTCTTTGAATGTCTTGCCACTGGTTGCAGTAAAAGTGTT
  CTGTATTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145051 unedited
  GGGGTCANAATTTGTATACGACTCACTATAGGGCCGGCGCGCGCAATTCGGCACGAGGCG
  ATTCAGGGAGGGAGCAACTGGAGCCTCAGGCCCTCCAGAGTAGTCTGCCTGACCACCCTG
  GAGCCCACAGAAGCCCAGGACGTCTCCCGCGAGGCCTCCCCGTGTGTGGCTGAGGATGGC
  TGAGCAGCAGGGCCGGGAGCTTGAGGCTGAGTGCCCCGTCTGCTGGAACCCCTTCAACAA
  CACGTTCCATACCCCCAAAATGCTGGATTGCTGCCACTCCTTCTGCGTGGAATGTCTGGC
  CCACCTCAGCCTTGTGACTCCAGCCCGGCGCCGCCTGCTGTGCCCACTCTGTCGCCAGCC
  CACAGTGCTGGCCTCAGGGCAGCCTGTCACTGACTTGCCCACGGACACTGCCATGCTCAC
  CCTGCTCCGCCTGGAGCCCCACCATGTCATCCTGGAAGGCCATCAGCTGTGCCTCAAGGA
  CCAGCCCAAGAGCCGCTACTTCCTGCGCCAGCCTCGAGTCTACACGCTGGACCTTGGCCC
  CCAGCCTGGGGGCCAGACTGGGCCGCCCCCAGACACGGCCTCTGCCACCGTGTCTACGCC
  CATCCTCATCCCCAGCCACCACTCATTGAGGGAGTGTTTCCGCAACCCTCAGTTCCGCAT
  CTTTGCCTACCTGATGGCCGTCATCCTCAGTGTCACTCTGTTGCTCATATTCTCCATCTT
  TTGGACCAAGCAGTTCCTTTGGGGTGTGGGGTGAGTGCTGTTCCCAGACAAGAAACCAAA
  CCTTTTTCGGTTGCTGCTGGGTATGGTGACTACNGAGCCTCATTTGGGATTGNCTTCCTT
  TGGAGGGNTGGTTATTTTACAATCCAGGNATTGTTCAGGCCATGTGTTTGCTTCTGGGAA
  CCATTTAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_145051
• Insert Size: 1274 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145051.2, NP_659488.1
• RefSeq Size: 1265 bp
• RefSeq ORF: 579 bp
• Locus ID: 138065
• UniProt ID: Q96D59
• Cytogenetics: 9q32
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Acts as a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:29507230). Triggers apoptosis in response to prolonged ER stress by mediating the polyubiquitination and subsequent proteasomal degradation of BCL2L1 (PubMed:29507230). May collaborate with FATE1 to restrain BIK protein levels thus regulating apoptotic signaling (PubMed:26567849).[UniProtKB/Swiss-Prot Function]