WDR79 (WRAP53) (NM_018081) Human Untagged Clone

CAT#: SC122873

WRAP53 (untagged)-Human WD repeat containing, antisense to TP53 (WRAP53), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WDR79
• Synonyms: DKCB3; TCAB1; WDR79
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018081 edited
  AAAGAATTGGCGTCCGCTGTTCGCCTCTCCTCCCGGGAGTCTTCTCCTACTCCCAGAAGA
  GGAGGGAAGCACAGTATGAAGACTTTGGAGACTCAACCGTTAGCTCCGGACTGCTGTCCT
  TCAGACCAGGACCCAGCTCCAGCCCATCCTTCTCCCCACGCTTCCCCGATGAATAAAAAT
  GCGGACTCTGAACTGATGCCACCGCCTCCCGAAAGGGGGGATCCGCCCCGGTTGTCCCCA
  GATCCTGTGGCTGGCTCAGCTGTGTCCCAGGAGCTACGGGAGGGGGACCCAGTTTCTCTC
  TCCACTCCCCTGGAAACAGAGTTTGGTTCCCCTAGTGAGTTGAGTCCTCGAATCGAGGAG
  CAAGAACTTTCTGAAAATACAAGCCTTCCTGCAGAAGAAGCAAACGGGAGCCTTTCTGAA
  GAAGAAGCGAACGGGCCAGAGTTGGGGTCTGGAAAAGCCATGGAAGATACCTCTGGGGAA
  CCCGCTGCAGAGGACGAGGGAGACACCGCTTGGAACTACAGCTTCTCCCAGCTGCCTCGA
  TTTCTCAGTGGTTCCTGGTCAGAGTTCAGCACCCAACCTGAGAACTTCTTGAAAGGCTGT
  AAGTGGGCTCCTGACGGTTCCTGCATCTTGACCAATAGTGCTGATAACATCTTGCGAATT
  TATAACCTGCCCCCAGAGCTGTACCATGAGGGGGAGCAGGTGGAATATGCAGAAATGGTC
  CCTGTCCTTCGAATGGTGGAAGGTGATACCATCTATGATTACTGCTGGTATTCTCTGATG
  TCCTCAGCCCAGCCAGACACCTCCTACGTGGCCAGCAGCAGCCGGGAGAACCCGATTCAT
  ATCTGGGACGCATTCACTGGAGAGCTCCGGGCTTCCTTTCGCGCCTACAACCACCTGGAT
  GAGCTGACGGCAGCCCATTCGCTCTGCTTCTCCCCGGATGGCTCCCAGCTCTTCTGTGGC
  TTCAACCGGACTGTGCGTGTTTTTTCCACGGCCCGGCCTGGCCGAGACTGCGAGGTCCGA
  GCCACATTTGCAAAAAAGCAGGGCCAGAGCGGCATCATCTCCTGCATAGCCTTCAGCCCA
  GCCCAGCCCCTCTATGCCTGTGGCTCCTACGGCCGCTCCCTGGGTCTGTATGCCTGGGAT
  GATGGCTCCCCTCTCGCCTTGCTGGGAGGGCACCAAGGGGGCATCACCCACCTCTGCTTT
  CATCCCGATGGCAACCGCTTCTTCTCAGGAGCCCGCAAGGATGCTGAGCTCCTGTGCTGG
  GATCTCCGGCAGTCTGGTTACCCACTGTGGTCCCTGGGTCGAGAGGTGACCACCAATCAG
  CGCATCTACTTCGATCTGGACCCGACCGGGCAGTTCCTAGTGAGTGGCAGCACGAGCGGG
  GCTGTCTCTGTGTGGGACACGGACGGGCCTGGCAATGATGGGAAGCCGGAGCCCGTGTTG
  AGTTTTCTGCCCCAGAAGGACTGCACCAATGGCGTGAGCCTGCACCCTAGCCTGCCTCTC
  CTGGCCACTGCCTCCGGTCAGCGTGTGTTTCCTGAGCCCACAGAGAGTGGGGACGAAGGA
  GAGGAGCTGGGCCTTCCCTTGCTCTCCACGCGCCACGTCCACCTTGAATGTCGGCTTCAG
  CTCTGGTGGTGTGGGGGGGCGCCAGACTCCAGCATCCCTGATGATCACCAGGGCGAGAAA
  GGGCAGGGAGGAACGGAGGGAGGTGTGGGTGAGCTGATATAAAAAGGTTTTTATGATAAA
  AAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018081 unedited
  NCCCCGTTTGGGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGAAAG
  AATGGCGTCCGCTGTTCGCCTCTCCTCCCGGGAGTCTTCTCCTACTCCCAGAAGAGGAGG
  GAAGCACAGTATGAAGACTTTGGAGACTCAACCGTTAGCTCCGGACTGCTGTCCTTCAGA
  CCAGGACCCAGCTCCAGCCCATCCTTCTCCCCACGCTTCCCCGATGAATAAAAATGCGGA
  CTCTGAACTGATGCCACCGCCTCCCGAAGGGGGGATCCGCCCCGGTTGTCCCCAGATCCT
  GTGGCTGGCTCAGCTGTGTCCCAGGAGCTACGGGAGGGGGACCCAGTTTCTCTCTCCACT
  CCCCTGGAAACAGAGTTTGGTTCCCCTAGTGAGTTGAGTCCTCGAATCGAGGAGCAAGAA
  CTTTCTGAAAATACAAGCCTTCCTGCAGAAGAAGCAAACGGGAGCCTTTCTGAAGAAGAA
  GCGAACGGGCCAGAGTTGGGGTCTGGAAAAGCCATGGAAGATACCTCTGGGGAACCCGCT
  GCAGAGGACGAGGGAGACACCGCTTGGAACTACAGCTTCTCCCAGCTGCCTCGATTTCTC
  AGTGGTTCCTGGTCAGAGTTCAGCACCCAACCTGAGAACTTCTTGAAAGGCTGTAAGTGG
  GCTCCTGACGGTTCCTGCATCTTGACCAATAGTGCTGATAACATCTTGCGAATTTATAAC
  CTGCCCCCAGAGCTGTACCATGAGGGGGAGCAGGTGGAATATGCAGAAATGGTCCCTGTC
  CTTCGAATGGTGGAAGGTGATACCATCTATGATTACTGCTGGTATTCTCTGATGTCCTCA
  GCCCAGCCAGACACCTCCTACGTGGCCAGCAGCAGCCGGGAGAACCCGATTCATATCTGG
  GACGCATTCACTGGAGAGCTCCGGGCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_018081
• Insert Size: 1766 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018081.1, NP_060551.1
• RefSeq Size: 1834 bp
• RefSeq ORF: 1647 bp
• Locus ID: 55135
• UniProt ID: Q9BUR4
• Cytogenetics: 17p13.1
• Gene Summary: This gene encodes an essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies, nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin, TERT and TERC, other components of active telomerase, and with small Cajal body RNAs (scaRNAs), which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript, that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene. [provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1-4 encode the same protein.