GNB3 (NM_002075) Human Untagged Clone

CAT#: SC122616

GNB3 (untagged)-Human guanine nucleotide binding protein (G protein), beta polypeptide 3 (GNB3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GNB3
• Synonyms: CSNB1H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002075 edited
  TGGGCAGGTGACGGGCGGGCGCGGGCGTCGCAGCTGAGGGAGTAAGGAGGCTCCCAGGAA
  CCGGAGCTGGAAACCCGGCCGAGGTCCAGCCAGAGCCCAAGAGCCAGAGTGACCCCTCGA
  CCTGTCAGCCATGGGGGAGATGGAGCAACTGCGTCAGGAAGCGGAGCAGCTCAAGAAGCA
  GATTGCAGATGCCAGGAAAGCCTGTGCTGACGTTACTCTGGCAGAGCTGGTGTCTGGCCT
  AGAGGTGGTGGGACGAGTCCAGATGCGGACGCGGCGGACGTTAAGGGGACACCTGGCCAA
  GATTTACGCCATGCACTGGGCCACTGATTCTAAGCTGCTGGTAAGTGCCTCGCAAGATGG
  GAAGCTGATCGTGTGGGACAGCTACACCACCAACAAGGTGCACGCCATCCCACTGCGCTC
  CTCCTGGGTCATGACCTGTGCCTATGCCCCATCAGGGAACTTTGTGGCATGTGGGGGGCT
  GGACAACATGTGTTCCATCTACAACCTCAAATCCCGTGAGGGCAATGTCAAGGTCAGCCG
  GGAGCTTTCTGCTCACACAGGTTATCTCTCCTGCTGCCGCTTCCTGGATGACAACAATAT
  TGTGACCAGCTCGGGGGACACCACGTGTGCCTTGTGGGACATTGAGACTGGGCAGCAGAA
  GACTGTATTTGTGGGACACACGGGTGACTGCATGAGCCTGGCTGTGTCTCCTGACTTCAA
  TCTCTTCATTTCGGGGGCCTGTGATGCCAGTGCCAAGCTCTGGGATGTGCGAGAGGGGAC
  CTGCCGTCAGACTTTCACTGGCCACGAGTCGGACATCAACGCCATCTGTTTCTTCCCCAA
  TGGAGAGGCCATCTGCACGGGCTCGGATGACGCTTCCTGCCGCTTGTTTGACCTGCGGGC
  AGACCAGGAGCTGATCTGCTTCTCCCACGAGAGCATCATCTGCAGCATCACGTCCGTGGC
  CTTCTCCCTCAGTGGCCGCCTACTATTCGCTGGCTACGACGACTTCAACTGCAATGTCTG
  GGACTCCATGAAGTCTGAGCGTGTGGGCATCCTCTCTGGCCACGATAACAGGGTGAGCTG
  CCTGGGAGTCACAGCTGACGGGATGGCTGTGGCCACAGGTTCCTGGGACAGCTTCCTCAA
  AATCTGGAACTGAGGAGGCTGGAGAAAGGGAAGTGGAAGGCAGTGAACACACTCAGCAGC
  CCCCTGCCCGACCCCATCTCATTCAGGTGTTCTCTTCTATATTCCGGGTGCCATTCCCAC
  TAAGCTTTCTCCTTTGAGGGCAGTGGGGAGCATGGGACTGTGCCTTTGGGAGGCAGCATC
  AGGGACACAGGGGCAAAGAACTGCCCCATCTCCTCCCATGGCCTTCCCTCCCCACAGTCC
  TCACAGCCTCTCCCTTAATGAGCAAGGACAACCTGCCCCTCCCCAGCCCTTTGCAGGCCC
  AGCAGACTTGAGTCTGAGGCCCCAGGCCCTAGGATTCCTCCCCCAGAGCCACTACCTTTG
  TCCAGGCCTGGGTGGTATAGGGCGTTTGGCCCTGTGACTATGGCTCTGGCACCACTAGGG
  TCCTGGCCCTCTTCTTATTCATGCTTTCTCCTTTTTCTACCTTTTTTTCTCTCCTAAGAC
  ACCTGCAATAAAGTGTAGCACCCTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002075 unedited
  NGGGAATGCGGTACGGAATTTGTNATACGACTCATATAGGCGGCCGCGNAATTCGCACGA
  GGTGGGCAGGTGACGGGCGGGCGCGGGCGTCGCAGCTGAGGGAGTAAGGAGGCTCCCAGG
  AACCGGAGCTGGAAACCCGGCCGAGGTCCAGCCAGAGCCCAAGAGCCAGAGTGACCCCTC
  GACCTGTCAGCCATGGGGGAGATGGAGCAACTGCGTCAGGAAGCGGAGCAGCTCAAGAAG
  CAGATTGCAGATGCCAGGAAAGCCTGTGCTGACGTTACTCTGGCAGAGCTGGTGTCTGGC
  CTAGAGGTGGTGGGACGAGTCCAGATGCGGACGCGGCGGACGTTAAGGGGACACCTGGCC
  AAGATTTACGCCATGCACTGGGCCACTGATTCTAAGCTGCTGGTAAGTGCCTCGCAAGAT
  GGGAAGCTGATCGTGTGGGACAGCTACACCACCAACAAGGTGCACGCCATCCCACTGCGC
  TCCTCCTGGGTCATGACCTGTGCCTATGCCCCATCAGGGAACTTTGTGGCATGTGGGGGG
  CTGGACAACATGTGTTCCATCTACAACCTCAAATCCCGTGAGGGCAATGTCAAGGTCAGC
  CGGGAGCTTTCTGCTCACACAGGTTATCTCTCCTGCTGCCGCTTCCTGGATGACAACAAT
  ATTGTGACCAGCTCGGGGGACACCACGTGTGCCTTGTGGGACATTGAGACTGGGCAGCAG
  AAGACTGTATTTGTGGGACACACGGGTGACTGCATGAGCCTGGCTGTGTCTCCTGACTTC
  AATCTCTTCATTTTCGGGGGCCTGTGATGCCAGTGCCAAGCTCTGGGATGTGCGAGAGGG
  GACCTGCCGTCAGACTTTCACTGGCCACGAGTCCGACATCAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002075
• Insert Size: 1724 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002075.2, NP_002066.1
• RefSeq Size: 1923 bp
• RefSeq ORF: 1023 bp
• Locus ID: 2784
• UniProt ID: P16520
• Cytogenetics: 12p13.31
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Taste transduction
• Gene Summary: Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).