POLE (BC021559) Human Untagged Clone

CAT#: SC122290

POLE (untagged)-Homo sapiens, polymerase (DNA directed), epsilon, clone MGC:31829 IMAGE:5014786, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLE
• Synonyms: DKFZp434F222; DNA polymerase epsilon catalytic subunit; FLJ21434; POLE1; polymerase (DNA directed), epsilon
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC021559 edited
  CTCTCTGCTTCATGACCCTGCCCTGCACCGCACACTCCACAACATGATGAAGAAGCTCTT
  CCTGCAGCTCATCGCTGAGTTCAAGCGCCTGGGGTCATCAGTCATCTACGCCAACTTCAA
  CCGCATCATCCTCTGTACAAAGAAGCGCCGTGTGGAAGATGCCATCGCTTACGTGGAGTA
  CATCACCAGCAGCATCCATTCAAAGGAGACCTTCCATTCTCTGACAATTTCTTTCTCTCG
  ATGCTGGGAATTTCTTCTCTGGATGGATCCATCTAACTATGGCGGAATCAAAGGAAAAGT
  TTCATCTCGTATTCACTGTGGACTGCAAGACTCCCAGAAAGCAGGGGGAGCAGAGGATGA
  GCAGGAAAATGAGGACGATGAGGAGGAAAGAGATGGGGAGGAGGAGGAAGAGGCGGAGGA
  ATCCAACGTGGAGGATTTACTGGAAAACAACTGGAACATTTTGCAGTTTTTGCCACAGGC
  AGCCTCCTGCCAGAACTACTTCCTCATGATTGTTTCAGCGTACATCGTGGCCGTGTACCA
  CTGCATGAAGGACGGGCTGAGGCGCAGTGCTCCAGGGAGCACCCCCGTGAGGAGGAGGGG
  GGCCAGCCAGCTCTCCCAGGAGGCCGAGGGGGCGGTCGGAGCCCTTCCCGGAATGATCAC
  CTTCTCTCAGGATTATGTCGCAAATGAGCTCACTCAGAGCTTCTTCACCATCACTCAGAA
  GATTCAGAAGAAAGTCACAGGCTCTCGGAACTCCACTGAGCTCTCAGAGATGTTTCCTGT
  CCTCCCCGGTTCCCACTTGCTGCTCAATAACCCTGCCCTGGAGTTCATCAAATACGTGTG
  CAAGGTGCTGTCCCTGGACACCAACATCACAAACCAGGTGAATAAGCTGAACCGAGACCT
  GCTTCGCCTGGTGGATGTCGGCGAGTTCTCCGAGGAGGCCCAGTTCCGAGACCCCTGCCG
  CTCCTACGTGCTTCCTGAGGTCATCTGCCGCAGCTGTAACTTCTGCCGCGACCTGGACCT
  GTGTAAAGACTCTTCCTTCTCAGAGGATGGGGCGGTCCTGCCTCAGTGGCTCTGCTCCAA
  CTGTCAGGCGCCCTACGACTCCTCTGCCATCGAGATGACGCTGGTGGAAGTTCTACAGAA
  GAAGCTGATGGCCTTCACCCTGCAGGACCTGGTCTGCCTGAAGTGCCGCGGGGTGAAGGA
  GACCAGCATGCCTGTGTACTGCAGCTGCGCGGGAGACTTCGCCCTCACCATCCACACCCA
  GGTCTTCATGGAACAGATCGGAATATTCCGGAACATTGCCCAGCACTACGGCATGTCGTA
  CCTCCTGGAGACCCTGGAGTGGCTGCTGCAGAAGAACCCACAGCTGGGCCATTAGCCAGC
  CCCGGGCCCCGGGTGCCTCTGCGTCCGTGCCAGGCCTCCTGATGCCAAGGCCACATCCCC
  GTGCTTCCAGTGACCAGACCACTGACCACCCTGACTGTCCAAACCTGTGACCCCAGGCCA
  GGGAACGGGGAGGAAACCAAAGAAAACCATTTTCAGGGAGCTCAGACGTCACAGGAGGGA
  GCGGGAGCAGGATGTGGCCCTGGCCTCGCCAGAGCACCTGAAGAAGCAGGCCGTGAGCGA
  GGCTGCGAGTGCCCTGGGCGCCGTTTCTCACGCAGTGAATGCTTTTCCAGGCCTCTGTTG
  CTTCCTGCACCACACCTGGTGGGGTGGGAGCGTCCTCTAGGTGCCCCTAGTTCTTTGTCC
  TGCCTCCCAGAGGGAGGAAAAGCCCCTGGGGGCTTCTGGCTCCCTGAGATTGGGCTCTGA
  GACGAGACGGGTTCCCAAGGCCCTGGTGGGGCTGGAGTCTCACCTGTTTGCATGGAGAAA
  TGGGCTGGCCCCACAGCCTCACAGGAGCAGTTTGTGGGCTGGTTTCCCCAGGAATCCAGA
  CCCTAACCCGTGAGAATCTGGATTTTGGCTTGTGAGCCCTGCTTATTTGGAGCCGGGTCT
  AGAGGGAACCCTCTATCAGCCTCAGGAAAACAAGACCTCTGTGCACCTCACTTTTGGCTC
  ACTGCAGCCCTTGTCCTTCACCTCCACACAGGACCAGCTGGAAGCAGAAAGAAGAAAGGC
  CAATTTCACAGGGCACCAAACAAGTATGAAATGTAAATCAGAAATGCAGACACCCCAGAC
  GAGAGCCTCACAGGAGGGAGGGGGCCCCACAGGCTCCCCAGGAGGCTCGTGTCTTTGGCC
  CAGAGCCAGCCTTAGTTTGTCCCTGCCATCTACTGTCTGAGGCCATCGCTGCTACACTTT
  GTTTTTATTTGTATTTCATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC021559 unedited
  GGGGTTCATATTTTGTAATACGACTCACTATAGGGCGGGCCGCGCAATTCGCACGAGGCT
  CTCTGCTTCATGACCCTGCCCTGCACCGCACACTCCACAACATGATGAAGAAGCTCTTCC
  TGCAGCTCATCGCTGAGTTCAAGCGCCTGGGGTCATCAGTCATCTACGCCAACTTCAACC
  GCATCATCCTCTGTACAAAGAAGCGCCGTGTGGAAGATGCCATCGCTTACGTGGAGTACA
  TCACCAGCAGCATCCATTCAAAGGAGACCTTCCATTCTCTGACAATTTCTTTCTCTCGAT
  GCTGGGAATTTCTTCTCTGGATGGATCCATCTAACTATGGCGGAATCAAAGGAAAAGTTT
  CATCTCGTATTCACTGTGGACTGCAAGACTCCCAGAAAGCAGGGGGAGCAGAGGATGAGC
  AGGAAAATGAGGACGATGAGGAGGAAAGAGATGGGGAGGAGGAGGAAGAGGCGGAGGAAT
  CCAACGTGGAGGATTTACTGGAAAACAACTGGAACATTTTGCAGTTTTTGCCACAGGCAG
  CCTCCTGCCAGAACTACTTCCTCATGATTGTTTCAGCGTACATCGTGGCCGTGTACCACT
  GCATGAAGGACGGGCTGAGGCGCAGTGCTCCAGGGAGCACCCCCGTGAGGAGGAGGGGGG
  CCAGCCAGCTCTCCCAGGAGGCCGAGGGGGCGGTCGGAGCCCTTCCCGGATGATCACCTT
  CTCTCAGGATTATGTCGCANATGAGCTCACTCAGAGCTTCTTCACCATCACTCAGAAGAT
  TCAGAAGAAAGTCACAGGCTCTCGGGACTCCACTGAGCTCTCAGAGATGTTTCCTGTCCT
  CCCGGTTCCCACTTGCTGCTCAATAACCCTGCCCTGGAGTTCATCAAATACGTGTGCAAG
  GTGCTGTCCA
  
• Restriction Sites: Please inquire
• ACCN: BC021559
• Insert Size: 2334 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021559.1, AAH21559.1
• RefSeq Size: 2334 bp
• Locus ID: 5426
• Cytogenetics: 12q24.33
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]