PLA2G1B (BC005386) Human Untagged Clone

CAT#: SC122227

PLA2G1B (untagged)-Homo sapiens, Similar to phospholipase A2, group IB (pancreas), clone MGC:12509 IMAGE:3950450, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLA2G1B
• Synonyms: MGC119834; MGC119835; phospholipase A2, group IB; phospholipase A2, group IB (pancreas); PLA2; PLA2A; PPLA2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC005386 edited
  AGTTCTTTTCTCACCTTGACTGCAAGATGAAACTCCTTGTGCTAGCTGTGCTGCTCACAG
  TGGCCGCCGCCGACAGCGGCATCAGCCCTCGGGCCGTGTGGCAGTTCCGCAAAATGATCA
  AGTGCGTGATCCCGGGGAGTGACCCCTTCTTGGAATACAACAACTACGGCTGCTACTGTG
  GCTTGGGGGGCTCAGGCACCCCCGTGGATGAACTGGACAAGCAAAAACAAAGAGTGTGAG
  GCCTTCATTTGCAACTGCGACCGCAACGCTGCCATCTGCTTTTCAAAAGCTCCATATAAC
  AAGGCACACAAGAACCTGGACACCAAGAAGTATTGTCAGAGTTGAATATCACCTCTCAAA
  AGCATCACCTCTATCTGCCTCATCTCACACTGTACTCTCCAATAAAGCACCTTGTTGAAA
  GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC005386 unedited
  NNGGTTTGAGTCAAATTTGTATACGACTCATATAGGCGGCACGCGAATCGGCCATTACGG
  CCGGGGAGTTCTTTTCTCACCTTGACTGCAAAATGAAACTCCTTGTGCTAGCTGTGCTGC
  TCACAGTGGCCGCCGCCGACAGCGGCATCAGCCCTCGGGCCGTGTGGCAGTTCCGCAAAA
  TGATCAAGTGCGTGATCCCGGGGAGTGACCCCTTCTTGGAATACAACAACTACGGCTGCT
  ACTGTGGCTTGGGGGGCTCATGCACCCCCGTGGATGAACTGGACAAGCAAAAACAAAGAG
  TGTGAGGCCTTCATTTGCAACTGCGACCGCAACGCTGCCATCTGCTTTTCAAAAGCTCCA
  TATAACAAGGCACACAAGAACCTGGACACCAAGAAGTATTGTCAGAGTTGAATATCACCT
  CTCAAAAGCATCACCTCTATCTGCCTCATCTCACACTGTACTCTCCAATAAAGCACCTTG
  TTGAAAGACAAANNANAAAAAAAAAAAAAAAAAAAAAAACATGTCGGCCGCCTCGGCCCT
  CGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCC
  TGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGC
  CTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTA
  TGGGGTGGAGGGGGNTGGGTATTGGAGCAAGGGGCAAGTTGGGAAAGACACCTGTAGGGC
  CTGCGGGGTCTATTGGGAACCAGCTGGATGCAGTGCNCAATCTTGGCTACTGCAATCTCG
  CCTCCTGGGTTCAGCGATT
  
• Restriction Sites: Please inquire
• ACCN: BC005386
• Insert Size: 453 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005386.1, AAH05386.1
• RefSeq Size: 453 bp
• Locus ID: 5319
• Cytogenetics: 12q24.31
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]