TBC1D32 (NM_152730) Human Untagged Clone

CAT#: SC121738

C6orf170 (untagged)-ORIGENE UNIQUE VARIANT 1 of Human chromosome 6 open reading frame 170 (C6orf170)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBC1D32
• Synonyms: BROMI; C6orf170; C6orf171
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_152730, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCATTTCTCCAGCGAGGACCAGGCGATGCTGCAGGCGATGCTGAGGCGGTTGTTCCAGAGCGTGA
  AGGAGAAAATCACGGGTGCCCCTTCCCTGGAGTGTGCCGAAGAGATTCTTTTACATCTGGAGGAAACTGA
  TGAAAATTTTCACAACTATGAATTTGTGAAATACCTCAGGCAGCATATAGGCAACACTTTGGGTTCTATG
  ATTGAAGAAGAAATGGAAAAATGCACATCTGATCGGAATCAGGGTGAAGAATGCGGCTATGATACAGTTG
  TACAGCAGGTCACTAAAAGAACTCAAGAATCTAAAGAGTACAAAGAAATGATGCATTACCTGAAGAACAT
  TATGATAGCTGTGGTCGAGTCTATGATTAACAAGTTTGAAGAAGATGAGACACGAAATCAAGAAAGGCAG
  AAAAAAATCCAAAAGGAGAAAAGCCATAGTTACCGCACAGACAATTGCTCTGATAGTGATTCATCATTGA
  ATCAGAGTTACAAATTTTGTCAAGGAAAATTACAATTGATTTTAGACCAGTTGGATCCTGGGCAACCTAA
  AGAGGTGAGATATGAAGCCTTGCAAACATTATGTTCAGCTCCTCCATCTGATGTCCTCAACTGTGAAAAT
  TGGACTACTCTCTGCGAAAAACTGACCGTGTCTCTTTCAGATCCTGATCCTGTGTTTAGTGACCGGATTT
  TAAAATTCTGTGCACAGACATTTTTGCTTTCTCCATTACATATGACCAAGGAAATTTATACAAGCTTAGC
  TAAGTATTTGGAGTCATACTTTCTTTCTAGGGAAAATCATATTCCTACTCTTTCAGCTGGTGTAGATATA
  ACTAATCCTAATATGACTCGCTTACTTAAAAAGGTTCGTCTTCTAAATGAATATCAGAAAGAAGCTCCAT
  CTTTCTGGATTCGTCATCCAGAGAAGTATATGGAAGAAATTGTGGAGAGTACTTTGTCCTTGTTAACAGT
  TAAACATAATCAAAGCCATGTTGTCTCACAAAAGATTTTGGATCCGATCTACTTTTTTGCATTAGTTGAT
  ACCAAGGCTGTGTGGTTCAAAAAGTGGATGCATGCACATTATAGCAGAACTACAGTATTAAGACTTCTTG
  AAACGAAATATAAGTCTCTGGTAACTACAGCCATTCAACAGTGTGTTCAGTACTTTGAAATGTGTAAGAC
  TAGGAAAGCTGATGAAACTTTGGGACATTCAAAGCATTGCAGAAACAAGCAGAAAACTTTCTACTACTTA
  GGACAAGAATTACAATATATTTATTTCATTCACTCACTGTGCCTTTTAGGAAGATTATTGATCTATAAAC
  AAGGCAGAAAACTATTTCCTATTAAGCTGAAGAATAAAAAAGGTTTGGTATCCCTCATAGATCTGCTTGT
  TCTTTTTACCCAACTTATCTATTACTCACCAAGTTGTCCAAAGATGACATCAGCTGCCCATTCAGAGAAT
  TACTCTCCTGCAAGTATGGTGACTGAAGTTCTGTGGATACTCAGTGATCAAAAAGAATGTGCAGTGGAAT
  GCTTATATAACAACATTGTAATAGAGACACTTCTTCAGCCTATTCACAATTTAATGAAAGGAAATGAGGC
  ATCTCCAAATTGCTCTGAGACAGCTTTAATTCATATAGCTGGTATTTTGGCAAGAATTGCATCTGTAGAA
  GAAGGGCTTATTTTACTCCTTTATGGAGCAAATATGAACTCTTCTGAAGAAAGTCCTACAGGTGCTCATA
  TAATTGCCCAGTTTTCGAAAAAACTTCTCGATGAAGATATTTCTATATTTTCTGGATCAGAAATGTTGCC
  TGTGGTTAAAGGAGCTTTTATTTCTGTGTGTCGTCACATATATAGTACATGTGAAGGTTTGCAGGTGTTA
  ATCACTTATAATTTGCATGAATCTATAGCAAAGGCATGGAAAAAGACAAGTTTGCTATCAGAAAGAATTC
  CTACTCCAGTAGAGGGTTCTGATTCTGTTTCTTCAGTAAGCCAGGAATCCCAAAACATTATGGCTTGGGA
  AGATAATTTGTTAGATGATTTACTACATTTTGCTGCCACCCCCAAAGGATTACTACTTCTTCAAAGAACA
  GGTGCTATCAATGAATGTGTGACATTTATATTCAATCGATATGCAAAAAAATTACAGGTCAGCAGGCATA
  AAAAATTTGGCTATGGAGTTTTGGTTACACGAGTGGCATCAACAGCAGCAGGTGGCATTGCACTAAAAAA
  GTCAGGGTTTATTAATGAACTTATAACTGAATTATGGTCCAATCTGGAATATGGAAGAGATGATGTTAGG
  GTAACCCATCCCAGAACTACTCCAGTGGATCCTATTGACCGAAGCTGTCAAAAGTCTTTTTTAGCACTGG
  TGAACTTGTTATCCTATCCTGCTATTTATGAGCTTGTAAGGAATCAAGATCTTCCTAATAAAACAGAATA
  TTCTCTTCGTGAAGTCCCAACATGTGTTATTGATATTATTGATAGACTTATAATTTTGAATTCTGAAGCT
  AAGATTCGTTCTTTATTCAACTATGAACAATCACATATCTTTGGTCTAAGGGACTTTATAATTGATGGCT
  TATCAGTGGAGAGAAATCATGTTCTTGTTAGAATAAATCTTGTTGGTGGGCCATTGGAACGGATTTTGCC
  TCCGAGGTTACTCGAAAAGAGTGATAATCCATATCCTTGGCCAATGTTTTCATCATATCCATTGCCAAAC
  TGCTATCTGTCAGACATTACAAGAAATGCTGGTATAAAACAAGACAATGATCTTGACAAGCTTTTATTAT
  GCCTCAAAATATCTGATAAACAAACTGAATGGATAGAAAACTGCCAAAGACAATTTTGCAAAATGATGAA
  AGCCAAACCTGATATAATCAGTGGAGAGGCCTTAATAGAATTACTTGAAAAATTTGTGCTTCATCTCACT
  GAAAGCCCATCTGAATGCTACTTCCCTTCAGTGGAGTATACAGCTACTGATGCAAATGTGAAGAATGAAA
  GTCTTTCATCTGTGCAGCAGCTTGGCATTAAAATGACTGTCAGGTATGGCAAATTCCTCAGTCTCTTAAA
  AGATGGTGCAGAAAATGATCTTACCTGGGTTTTAAAGCATTGTGAGAGATTCCTGAAACAGCAGCAAACT
  TCCATAAAATCTTCTCTTCTCTGCCTGCAAGGGAATTATGCTGGCCATGACTGGTTTGTATCTTCTCTGT
  TCATGATAATGTTGGGAGACAAAGAAAAAACATTCCAATTTCTTCATCAATTCTCCAGGCTTCTGACTTC
  TGCTTTTCTTTGGTTGCCAAGGCTACATATTTCTAGTTACCTTCCTAATGACACTGTAGAATCTGGCATC
  CATCCAGTATATTTTTGCAGCACCCATTATATTGAAATGCTACTGAAGGCTGAGTTGCCTCTTGTGTTTT
  CAGCTTTTCACATGTCTGGTTTTGCACCATCACAGATTTGCCTGCAATGGATAACCCAGTGTTTTTGGAA
  TTACTTAGATTGGATAGAAATCTGCCATTATATTGCTACTTGTGTTTTCCTTGGTCCTGATTATCAAGTG
  TATATCTGTATAGCTGTATTCAAACATTTACAGCAAGACATTCTACAGCACACTCAGACTCAAGATCTGC
  AAGTTTTCCTAAAAGAAGAAGCACTGCATGGGTTTCGAGTGAGTGATTATTTTGAATACATGGAAATTTT
  GGAACAAAACTACCGAACAGTGCTGCTGAGAGACATGCGGAACATTAGACTGCAGAGCACATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152730 unedited
  AAAGCGTTCANCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTGG
  ACGTTTGATTCCAACAGATGGCCCATTTCTCCAGCGAGGACCAGGCGATGCTGCAGGCGA
  TGCTGAGGCGGTTGTTCCAGAGCGTGAAGGAGAAAATCACGGGTGCCCCTTCCCTGGAGT
  GTGCCGAAGAGATTCTTTTACATCTGGAGGAAACTGATGAAAATTTTCACAACTATGAAT
  TTGTGAAATACCTCAGGCAGCATATAGGCAACACTTTGGGTTCTATGATTGAAGAAGAAA
  TGGAAAAATGCACATCTGATCGGAATCAGGGTGAAGAATGCGGCTATGATACAGTTGTAC
  AGCAGGTCACTAAAAGAACTCAAGAATCTAAAGAGTACAAAGAAATGATGCATTACCTGA
  AGAACATTATGATAGCTGTGGTCGAGTCTATGATTAACAAGTTTGAAGAAGATGAGACAC
  GAAATCAAGAAAGGCAGAAAAAAATCCAAAAGGAGAAAAGCCATAGTTACCGCACAGACA
  ATTGCTCTGATAGTGATTCATCATTGAATCAGAGTTACAAATTTTGTCAAGGAAAATTAC
  AATTGATTTTAGACCAGTTGGATCCTGGGCAACCTAAAGAGGTGAGATATGAAGCCTTGC
  AAACATTATGTTCAGCTCCTCCATCTGATGTCCTCAACTGTGAAAATTGGACTACTCTCT
  GTGAAAAACTGACCGTGTCTCTTTCAGATCCTGATCCTGTGTTTAGTGACCGGATTTTAA
  ATTCTGTGCACAGACATTTTTGCTTTCTCCATTACATATGACCAAGGGAAATTATACAAG
  CTTAGCTAAGTATTTGGAGTCATACTTTCTTTTNCTAGGAAATATATTCCTACTCTTTCA
  GCTGGNG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152730 unedited
  NNACTGGGGNAGGGGCTCACAGGGNATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGA
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTCTCAAAACTCAGTTTTATTGACTTCCACCATTTCTTGTAATGTCTGACAAATAGCATT
  TTGGCAATGGATATGATGAAAACATTGGCCAAGGATAGGGATTACCACCCTTTTCGAGTA
  ACCCCGGAGGCAAAATCCGTTCCAAGGGCCCACCAACAAGATTTATTCTAACAAAAACAT
  GATTTCCCCCCACTGATAACCCATCAATTATAAAGCCCCTGGGGCCCCCAAAAATCCCCA
  AAATATTTTCTTCTTGAGCATTTAATAACATTTCAAATACCTGATACTGACCCTCTAACA
  GGAGAAAAGTGTCCAGATCACAGCATATCACACTTAATAACCTTAAACCAAAGATATGTG
  ATTGTTCAAAGTTGAAAAAAAAACCAATCTTACCTTCAAAATTCAAAATTATAAGTCTAT
  CAATAATATCAATAACCCATGTTGGGACTTCCCGAAGAAAATATTCTGTTTTATTAGGAA
  GATCCTTATTCCTTACCACCCCAAAAATACCGGGGAAGGAAAACAAGTTCCCCCACTGCC
  AAAAAAAAACTTTTGCACCGCTTCGGCCAAAGGCCCCCATGGGGAAATTTCCGGGGGTGG
  GTTACCCCCAACAGCCCCTTCACCAAAATCCCACAAAGCGCCCCAGACTTCACGTGAAAA
  ATCCATCAGAGAGCCCCGAACCCCCTTTCATCGCCACGCCCACCCGGCCCGCGGCCGGGA
  CCCCCCCGCCCAGAACCCGAACAACGCCAACAAAATTCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152730
• Insert Size: 3000 bp
• OTI Disclaimer: The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
• OTI Annotation: This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152730.3, NP_689943.3
• RefSeq Size: 3431 bp
• RefSeq ORF: 2934 bp
• Locus ID: 221322
• UniProt ID: Q96NH3
• Cytogenetics: 6q22.31
• Gene Summary: This gene encodes a TBC-domain containing protein. Studies of a similar protein in mouse and zebrafish suggest that the encoded protein is involved in sonic hedgehog signaling, and that it interacts with and stabilizes cell cycle-related kinase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the supported protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.