Ribonuclease Inhibitor (RNH1) (NM_203386) Human Untagged Clone

CAT#: SC121083

RNH1 (untagged)-Human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNH1
• Synonyms: RAI; RNH
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_203386, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCTGGACATCCAGAGCCTGGACATCCAGTGTGAGGAGCTGAGCGACGCTAGATGGGCCGAGCTCC
  TCCCTCTGCTCCAGCAGTGCCAAGTGGTCAGGCTGGACGACTGTGGCCTCACGGAAGCACGGTGCAAGGA
  CATCAGCTCTGCACTTCGAGTCAACCCTGCACTGGCAGAGCTCAACCTGCGCAGCAACGAGCTGGGCGAT
  GTCGGCGTGCATTGCGTGCTCCAGGGCCTGCAGACCCCCTCCTGCAAGATCCAGAAGCTGAGCCTCCAGA
  ACTGCTGCCTGACGGGGGCCGGCTGCGGGGTCCTGTCCAGCACACTACGCACCCTGCCCACCCTGCAGGA
  GCTGCACCTCAGCGACAACCTCTTGGGGGATGCGGGCCTGCAGCTGCTCTGCGAAGGACTCCTGGACCCC
  CAGTGCCGCCTGGAAAAGCTGCAGCTGGAGTATTGCAGCCTCTCGGCTGCCAGCTGCGAGCCCCTGGCCT
  CCGTGCTCAGGGCCAAGCCGGACTTCAAGGAGCTCACGGTTAGCAACAACGACATCAATGAGGCTGGCGT
  CCGTGTGCTGTGCCAGGGCCTGAAGGACTCCCCCTGCCAGCTGGAGGCGCTCAAGCTGGAGAGCTGCGGT
  GTGACATCAGACAACTGCCGGGACCTGTGCGGCATTGTGGCCTCCAAGGCCTCGCTGCGGGAGCTGGCCC
  TGGGCAGCAACAAGCTGGGTGATGTGGGCATGGCGGAGCTGTGCCCAGGGCTGCTCCACCCCAGCTCCAG
  GCTCAGGACCCTGTGGATCTGGGAGTGTGGCATCACTGCCAAGGGCTGCGGGGATCTGTGCCGTGTCCTC
  AGGGCCAAGGAGAGCCTGAAGGAGCTCAGCCTGGCCGGCAACGAGCTGGGGGATGAGGGTGCCCGACTGC
  TGTGTGAGACCCTGCTGGAACCTGGCTGCCAGCTGGAGTCGCTGTGGGTGAAGTCCTGCAGCTTCACAGC
  CGCCTGCTGCTCCCACTTCAGCTCAGTGCTGGCCCAGAACAGGTTTCTCCTGGAGCTACAGATAAGCAAC
  AACAGGCTGGAGGATGCGGGCGTGCGGGAGCTGTGCCAGGGCCTGGGCCAGCCTGGCTCTGTGCTGCGGG
  TGCTCTGGTTGGCCGACTGCGATGTGAGTGACAGCAGCTGCAGCAGCCTCGCCGCAACCCTGTTGGCCAA
  CCACAGCCTGCGTGAGCTGGACCTCAGCAACAACTGCCTGGGGGACGCCGGCATCCTGCAGCTGGTGGAG
  AGCGTCCGGCAGCCGGGCTGCCTCCTGGAGCAGCTGGTCCTGTACGACATTTACTGGTCTGAGGAGATGG
  AGGACCGGCTGCAGGCCCTGGAGAAGGACAAGCCATCCCTGAGGGTCATCTCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_203386 unedited
  GGAATTTCGGAATTTGTAATACGACTCACTATAGGGCGCGCCGCGAATCGGCACCANATC
  GTCAACCTTTTCCACCTTCTCCAGTCTGGGGGCAAAAGGGGTGTCTCAGGCCACTCTTCA
  CCTCCACCATGAGCCTGGACATCCAGAGCCTGGACATCCAGTGTGAGGAGCTGAGCGACG
  CTAGATGGGCCGAGCTCCTCCCTCTGCTCCAGCAGTGCCAAGTGGTCAGGCTGGACGACT
  GTGGGCTCACGGAAGCACGGTGCAAGGACATCAGCTCTGCACTTCGAGTCAACCCTGCAC
  TGGCAGAGCTCAACCTGCGCAGCAACGAGCTGGGCGATGTCGGCGTGCATTGCGTGCTCC
  AGGGCCTGCAGACCCCCTCCTGCAAGATCCAGAAGCTGAGCCTCCAGAACTGCTGCCTGA
  CGGGGGCCGGCTGCGGGGTCCTGTCCAGCACACTACGCACCCTGCCCACCCTGCAGGAGC
  TGCACCTCAGCGACAACCTCTTGGGGGATGCGGGCCTGCAGCTGCTCTGCGAAGGACTCC
  TGGACCCCCAGTGCCGCCTGGAAAAGCTGCAGCTGGAGTATTGCAGCCCTCTCGGCTGCC
  AGCTGCGAGCCCCTGGCCTCCGTGCTCAGGGCCAAGCCGGACTTCAAGGAGCTCACGGTT
  AGCAACAACGACATCAATGAGGCTGGCGTCCGTGTGCTGTGCCAGGGCCTGAAGGACTCC
  CCCTGCCAGCTGGAAGCGCTCAAGCTGGAGAGCTGCGGTGTGACATTAGACAACTGCCCG
  GACCTGTGTGGCATTGCGGCCTCCACGCCCTCCTGCGGGAGCTGGCCCTGGGCCCAACAA
  GACTGGTGATGTGGGCATGGCGGACCTGTGCCAGGGTTGGTCCCCCCAGTCCAGGCTAAG
  ACCCGTGGATCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_203386
• Insert Size: 1550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203386.1, NP_976320.1
• RefSeq Size: 1937 bp
• RefSeq ORF: 1386 bp
• Locus ID: 6050
• UniProt ID: P13489
• Cytogenetics: 11p15.5
• Gene Summary: Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]
  Transcript Variant: This variant (5) contains a different exon and uses a different splice site in its 5' UTR, compared to variant 1. All eight variants of this gene encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.