PIP5K3 (PIKFYVE) (NM_015040) Human Untagged Clone

CAT#: SC121037

PIKFYVE (untagged)-Human phosphoinositide kinase, FYVE finger containing (PIKFYVE), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIP5K3
• Synonyms: CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC121037 sequence for NM_015040 edited (data generated by NextGen Sequencing)
  ATGGCCACAGATGATAAGACGTCCCCAACACTGGACTCTGCTAATGATTTGCCTCGATCT
  CCTACTAGTCCTTCTCATCTCACACACTTTAAACCTTTGACTCCTGATCAAGATGAGCCC
  CCTTTTAAATCAGCTTATAGTTCTTTTGTAAATCTCTTTCGTTTTAACAAAGAGAGAGCA
  GAAGGAGGCCAGGGAGAACAGCAGCCTTTGAGTGGAAGTTGGACCAGCCCTCAGCTCCCT
  TCGAGGACACAGTCTGTTAGGTCACCCACACCTTATAAAAAGCAGCTTAATGAGGAACTC
  CAGCGGCGCTCTTCAGCATTAGACACAAGAAGGAAAGCAGAACCTACCTTTGGAGGTCAT
  GACCCTCGTACAGCTGTTCAGCTTCGAAGCCTCAGCACAGTATTAAAACGCCTCAAGGAA
  ATCATGGAGGGGAAAAGCCAGGATAGTGACCTGAAACAATACTGGATGCCAGATAGCCAA
  TGTAAAGAGTGCTATGACTGTAGTGAGAAATTTACAACCTTTAGGCGCAGACACCATTGC
  CGACTATGTGGGCAGATTTTCTGCAGTCGTTGCTGTAATCAAGAAATCCCTGGAAAATTT
  ATGGGCTATACAGGAGACCTCCGAGCTTGCACATATTGTAGAAAAATAGCCTTAAGTTAT
  GCTCATTCCACAGACAGTAATTCTATTGGGGAAGACTTGAATGCTCTTTCAGATTCTGCT
  TGCTCTGTGTCTGTGCTTGATCCAAGTGAACCCCGAACACCTGTTGGGAGTAGGAAAGCC
  AGCCGTAACATATTTTTAGAGGATGATTTGGCCTGGCAAAGTTTGATTCATCCAGATTCC
  TCAAATACTCCTCTTTCAACAAGACTTGTATCTGTGCAAGAGGATGCTGGGAAATCTCCT
  GCTCGAAATAGATCAGCCAGCATTACTAACCTGTCACTGGATAGATCTGGTTCTCCTATG
  GTACCTTCATATGAGACATCTGTCAGTCCCCAGGCTAACCGAACATATGTTAGGACAGAG
  ACCACTGAGGATGAACGCAAAATTCTTCTGGACAGTGTGCAGTTAAAAGACCTGTGGAAA
  AAAATCTGCCATCACAGCAGTGGAATGGAGTTTCAGGATCACCGCTACTGGTTGAGAACG
  CATCCCAACTGCATTGTAGGAAAGGAATTAGTCAACTGGCTAATCCGAAATGGGCATATT
  GCCACAAGGGCACAAGCTATAGCAATTGGACAAGCAATGGTTGATGGACGTTGGCTGGAT
  TGTGTTAGTCATCACGACCAGCTTTTCAGAGATGAGTATGCGCTGTATAGACCACTGCAG
  AGTACAGAATTTTCTGAGACGCCTTCTCCCGACAGTGACTCAGTGAACTCCGTGGAAGGA
  CACTCTGAGCCATCCTGGTTTAAAGACATAAAGTTTGATGACAGTGACACAGAACAGATA
  GCTGAAGAAGGTGACGATAATTTGGCTAATTCTGCCAGTCCTAGCAAGCGCACATCAGTC
  AGCAGTTTCCAGTCCACAGTGGACAGTGACTCAGCCGCTTCTATCAGCCTGAACGTGGAG
  CTGGACAACGTGAACTTCCATATCAAGAAGCCCTCCAAGTACCCACATGTGCCCCCTCAC
  CCTGCTGACCAAAAAGAGTATTTGATTTCTGACACTGGAGGACAACAGCTCTCAATAAGT
  GACGCTTTCATCAAAGAATCCTTATTTAATCGCCGAGTAGAGGAAAAATCCAAAGAGCTG
  CCTTTCACACCTTTGGGCTGGCATCATAACAACCTGGAGCTCCTGAGGGAGGAGAATGGG
  GAGAAACAAGCCATGGAGAGGTTGCTTTCAGCTAATCATAACCACATGATGGCACTACTC
  CAGCAGTTGCTCCATAGTGACTCACTGTCATCATCTTGGAGGGACATCATCGTGTCATTG
  GTCTGCCAGGTTGTTCAGACAGTCCGACCTGATGTCAAGAACCAGGATGATGACATGGAT
  ATCCGTCAGTTTGTCCACATCAAAAAAATCCCAGGTGGAAAGAAGTTTGATTCTGTGGTT
  GTCAATGGCTTTGTTTGTACCAAGAACATTGCACATAAAAAGATGAATTCTTGTATTAAA
  AACCCTAAAATTCTTCTGTTGAAGTGTTCCATTGAGTATCTCTACAGAGAAGAAACTAAG
  TTTACTTGCATTGATCCTATTGTGCTTCAGGAAAGGGAATTCTTGAAGAATTATGTCCAG
  CGAATAGTTGATGTTCGACCCACCTTGGTTCTTGTTGAGAAAACAGTGTCTCGGATTGCC
  CAGGACATGTTATTGGAACATGGCATTACTTTGGTCATTAATGTAAAGTCACAAGTTTTG
  GAACGAATCAGTCGAATGACCCAAGGTGATTTAGTGATGTCAATGGACCAGCTGCTTACG
  AAACCACACCTGGGCACTTGTCACAAATTTTATATGCAGATATTTCAGTTGCCTAATGAA
  CAAACCAAGACACTGATGTTTTTTGAAGGTTGTCCACAGCACCTAGGCTGTACAATCAAG
  CTAAGAGGAGGCTCTGATTATGAGCTGGCTCGAGTTAAGGAGATCCTAATATTTATGATC
  TGTGTTGCTTATCATTCTCAACTAGAAATATCCTTTCTCATGGATGAATTTGCTATGCCT
  CCCACATTAATGCAAAACCCTTCATTCCATTCCCTGATTGAGGGACGAGGGCATGAGGGG
  GCTGTCCAAGAGCAGTACGGTGGAGGTTCCATCCCCTGGGATCCTGACATCCCTCCTGAG
  TCTCTGCCCTGTGATGATAGCAGTTTGCTGGAATCGAGGATTGTGTTTGAGAAGGGTGAG
  CAGGAAAATAAAAATCTTCCGCAGGCTGTTGCCTCTGTGAAGCATCAAGAACATAGCACA
  ACAGCTTGCCCGGCGGGTCTCCCTTGTGCTTTCTTTGCACCTGTACCGGAATCATTGTTG
  CCACTCCCTGTGGATGACCAACAAGATGCTTTAGGCAGCGAGCTGCCAGAGAGTTTGCAG
  CAAACAGTTGTGCTGCAGGATCCCAAAAGCCAGATAAGAGCCTTTAGAGACCCTCTACAG
  GATGACACTGGATTATATGTTACTGAGGAAGTCACCTCCTCTGAAGATAAACGAAAGACT
  TATTCTTTGGCCTTTAAGCAGGAATTAAAAGATGTGATCCTCTGTATCTCCCCAGTAATC
  ACATTCCGAGAACCCTTTCTTTTAACTGAAAAGGGGATGAGATGCTCTACCCGAGATTAT
  TTTGCAGAGCAGGTTTACTGGTCTCCTCTCCTCAATAAAGAATTCAAAGAAATGGAGAAC
  AGGAGGAAGAAACAGCTGCTCAGGGATCTCTCTGGACTTCAGGGCATGAATGGAAGTATT
  CAGGCCAAGTCTATTCAAGTCTTACCCTCACATGAGCTAGTGAGCACTAGAATTGCTGAG
  CATCTGGGCGATAGCCAGAGCTTGGGTAGAATGCTGGCCGATTATCGAGCCAGAGGAGGA
  AGAATTCAGCCCAAAAATTCAGACCCTTTTGCTCATTCAAAGGATGCATCAAGTACTTCA
  AGTGGCAAATCAGGAAGCAAAAACGAGGGTGATGAAGAGAGAGGGCTTATTCTGAGTGAT
  GCTGTGTGGTCAACAAAGGTGGACTGTCTGAATCCCATTAATCACCAGAGACTTTGTGTG
  CTCTTCAGCAGCTCTTCTGCCCAGTCCAGCAATGCTCCTAGTGCCTGTGTCAGTCCTTGG
  ATTGTAACAATGGAATTTTATGGAAAGAATGATCTTACATTAGGAATATTTTTAGAGAGA
  TACTGTTTCAGGCCTTCTTATCAGTGTCCAAGCATGTTCTGTGATACCCCCATGGTACAT
  CATATTCGGCGCTTTGTTCATGGCCAAGGCTGTGTGCAGATAATCCTGAAGGAGTTGGAT
  TCTCCAGTACCTGGATATCAGCATACAATTCTTACATATTCCTGGTGTAGAATCTGCAAA
  CAGGTAACACCAGTTGTTGCTCTTTCCAATGAGTCCTGGTCTATGTCATTTGCAAAATAC
  CTTGAACTTAGGTTTTATGGGCACCAGTATACTCGCAGAGCCAACGCTGAGCCCTGTGGT
  CACTCCATCCATCATGATTATCACCAGTATTTCTCCTATAACCAGATGGTGGCGTCTTTC
  AGTTATTCTCCCATTCGGCTTCTTGAAGTATGTGTTCCACTCCCCAAAATATTCATTAAG
  CGTCAGGCCCCATTAAAAGTGTCCCTTCTTCAGGATCTGAAGGACTTCTTTCAAAAAGTT
  TCACAGGTATATGTTGCCATTGATGAAAGACTTGCATCTTTGAAAACTGATACATTTAGT
  AAAACAAGAGAGGAAAAAATGGAAGATATTTTTGCACAGAAAGAGATGGAAGAAGGTGAG
  TTCAAGAACTGGATTGAGAAGATGCAAGCAAGGCTCATGTCTTCCTCTGTAGATACCCCT
  CAGCAACTGCAGTCGGTCTTTGAGTCACTCATTGCCAAGAAACAAAGTCTCTGTGAAGTG
  CTGCAAGCTTGGAATAACAGGTTGCAGGACCTTTTCCAACAGGAAAAGGGTAGAAAGAGA
  CCTTCAGTTCCTCCAAGTCCTGGAAGACTGAGACAAGGGGAAGAAAGCAAGATAAGTGCG
  ATGGATGCATCTCCACGGAATATTTCTCCAGGACTTCAGAATGGAGAAAAAGAGGATCGC
  TTCTTAACAACTTTGTCCAGCCAGAGCTCCACCAGTTCTACTCATCTCCAATTGCCTACG
  CCACCTGAAGTCATGTCTGAACAGTCAGTGGGAGGGCCCCCTGAGCTAGATACAGCCAGC
  AGTTCCGAAGATGTGTTTGATGGGCATTTGCTGGGATCCACAGACAGCCAAGTGAAGGAA
  AAGTCAACCATGAAAGCCATCTTTGCAAATTTGCTTCCAGGAAATAGCTATAATCCTATT
  CCATTTCCTTTTGATCCAGATAAACACTACTTAATGTATGAACATGAACGAGTGCCCATT
  GCAGTCTGCGAGAAGGAACCCAGCTCCATCATTGCTTTTGCTCTCAGTTGTAAAGAATAC
  CGAAATGCCTTAGAGGAATTGTCTAAAGCGACTCAGTGGAACAGTGCCGAAGAAGGGCTT
  CCAACAAATAGTACTTCAGATAGCAGACCAAAGAGTAGCAGCCCTATCAGATTACCTGAA
  ATGAGTGGAGGACAGACAAATCGTACAACAGAAACAGAACCACAACCAACCAAAAAGGCT
  TCTGGAATGTTGTCCTTCTTCAGAGGGACAGCAGGGAAAAGCCCCGATCTCTCTTCCCAG
  AAGAGAGAGACCTTACGTGGAGCAGATAGTGCTTACTACCAGGTTGGGCAGACAGGCAAG
  GAGGGGACCGAGAATCAAGGCGTTGAGCCTCAAGATGAAGTAGATGGAGGAGATACGCAA
  AAGAAGCAACTCATAAATCCTCATGTGGAACTTCAATTTTCAGATGCTAATGCCAAGTTT
  TACTGTCGGCTCTACTATGCGGGAGAGTTTCATAAGATGCGTGAAGTGATTCTGGACAGC
  AGTGAGGAAGATTTCATTCGTTCCCTCTCCCACTCATCACCCTGGCAGGCCCGGGGAGGC
  AAATCAGGAGCTGCCTTCTATGCAACTGAGGATGATAGATTTATTTTGAAGCAAATGCCT
  CGTCTGGAAGTCCAGTCCTTCCTCGACTTTGCACCACATTACTTCAATTATATTACAAAT
  GCTGTTCAACAAAAGAGGCCCACGGCGTTGGCCAAAATTCTTGGAGTTTACAGAATTGGT
  TATAAGAACTCTCAGAACAACACTGAGAAGAAGTTAGATCTCCTTGTCATGGAAAATCTT
  TTCTACGGGAGAAAGATGGCACAGGTTTTTGATTTGAAGGGCTCTCTTAGGAATCGGAAT
  GTAAAAACTGACACTGGAAAAGAGAGTTGTGATGTGGTCCTGCTAGATGAAAATCTCCTA
  AAGATGGTTCGAGACAACCCTCTATATATTCGTTCTCATTCCAAAGCTGTGCTGAGAACC
  TCGATCCATAGTGACTCCCATTTCCTTTCTAGCCACCTCATTATAGATTATTCTTTGCTG
  GTTGGGCGAGATGATACTAGCAATGAGCTAGTAGTTGGAATTATAGATTATATTCGAACA
  TTTACATGGGACAAAAAGCTTGAGATGGTTGTGAAATCAACAGGAATTTTAGGTGGACAA
  GGTAAAATGCCAACAGTGGTGTCTCCGGAGTTGTACAGGACTAGGTTTTGTGAGGCAATG
  GACAAGTATTTCCTAATGGTACCAGACCACTGGACAGGCTTGGGTCTGAATTGCTGA
  
  Clone variation with respect to NM_015040.3
  2087 g=>a;2106 c=>t;2795 t=>c;2984 a=>t;2993 c=>g;3547 c=>a;3564 t=>c;5334 g=>a;5397 a=>g;5526 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015040 unedited
  GGGTGCAAAATTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGCTT
  CCTGCCGCAACCGTCCGCGGCCTGAGGAGCCCACCGCCGCTCTCGGGGGCCGACTTCCGG
  GGGCTGAGCCGTTGAAGCGGAGGCTGGGGCGGGGGGCAGCCGGCGCGGCCGGGGCAGGAG
  GCGCAGACTCATGAAATGGCCACAGATGATAAGACGTCCCCAACACTGGACTCTGCTAAT
  GATTTGCCTCGATCTCCTACTAGTCCTTCTCATCTCACACACTTTAAACCTTTGACTCCT
  GATCAAGATGAGCCCCCTTTTAAATCAGCTTATAGTTCTTTTGTAAATCTCTTTCGTTTT
  AACAAAGAGAGAGCAGAAGGAGGCCAGGGAGAACAGCAGCCTTTGAGTGGAAGTTGGACC
  AGCCCTCAGCTCCCTTCGAGGACACAGTCTGTTAGGTCACCCACACCTTATAAAAAGCAG
  CTTAATGAGGAACTCCAGCGGCGCTCTTCAGCATTAGACACAAGAAGGAAAGCAGAACCT
  ACCTTTGGAGGTCATGACCCTCGTACAGCTGTTCAGCTTCGAAGCCTCAGCACAGTATTA
  AAACGCCTCAAGGAAATCATGGAGGGGAAAAGCCAGGATAGTGACCTGAAACAAACTGGA
  TGCCAGATAGCCAATGTAAAGAGTGCTATGACTGTAGTGAGAAATTACAACCTTTTAGCG
  CAGACACCATTGCCGACTATGTGGGCAGATTNTCTGCAGTCGTTGCTGTAATCAAGAAAT
  CCCCTGGAAAATTATGGGCTATACANGAGACCTNCGAGCTGCACATATTGTAGAAAATAG
  CCTTAGTATGCTCATTCCACAGACAGTATCTATGGGGGAAGACTGATGCTCTTCAGATCT
  GCTGCTCTNGGCTGGCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015040
• Insert Size: 6300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015040.2, NP_055855.1
• RefSeq Size: 9908 bp
• RefSeq ORF: 6297 bp
• Locus ID: 200576
• UniProt ID: Q9Y2I7
• Cytogenetics: 2q34
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• Gene Summary: Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).