Cytochrome P450 4A (CYP4A11) (NM_000778) Human Untagged Clone

CAT#: SC120814

CYP4A11 (untagged)-Human cytochrome P450, family 4, subfamily A, polypeptide 11 (CYP4A11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytochrome P450 4A
• Synonyms: CP4Y; CYP4A2; CYP4AII; CYPIVA11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120814 sequence for NM_000778 edited (data generated by NextGen Sequencing)
  ATGAGTGTCTCTGTGCTGAGCCCCAGCAGACTCCTGGGTGATGTCTCTGGAATCCTCCAA
  GCGGCCTCCCTGCTCATTCTGCTTCTGCTGCTGATCAAGGCAGYTCAGCTCTACCTGCAY
  AGGCAGTGGCTGCTCAAAGCCCTCCAGCAGTTCCCGTGCCCTCCCTCCCACTGGCTCTTC
  GGGCACATCCAGGAGCTCCAACAGGACCAGGAGCTACAACGGATTCAGAAATGGGTGGAG
  ACATTCCCAAGTGCCTGTCCTYATTGGMTATGGGGAGGCAAAGTTCGTGTCCAGCTCTAT
  GACCCTGACTATATGAAGGTGATTCTGGGGAGATCAGACCCGAAATCCCATGGWTCCTAC
  ARATTCCTGGCTCCAYGGATTGGGTACGGCTTGCTCCTGTTGAATGGGCAGACATGGTTC
  CAGCATCGACGGATGCTGACCCCAGCCTTCCACTATGACATCCTGAAGCCCTATGTGGGG
  CTCATGGCAGACTCTGTACGAGTGATGCTGGACAAATGGGAAGAGCTCCTTGGCCAGGAT
  TCCCCTCTGGAGGTCTTTCAGCACGTCTCCTTGATGACCCTGGACACCATCATGAAGWGT
  GCCTTCAGCCATCAGGGCAGCATCCAGGTGGACAGGAATTCTCAGTCCTACATACAGGCC
  ATTAGTGACCTGAACAACCTGGTTTTTTCCCGTGTGAGGAATGCCTTTCACCAGAATGAC
  ACCATCTACAGCCTGACCTCTGCTGGCCGCTGGACACACCGCGCCTGCCAGCTGGCCCAT
  CAGCACACAGACCAAGTGATCCAACTGAGGAAGGCTCAACTACAGAAGGAGGGGGAGCTG
  GAGAAGATCAAGAGGAAGAGGCATTTGGATTTTCTGGATATCCTCCTCTTGGCCAAAATG
  GAGAATGGGAGCATCTTGTCAGACAAGGACCTCCGTGCTGAGGTGGACACGTTCATGTTT
  GAGGGCCATGACACCACAGCCAGTGGGATCTCCTGGATCCTCTATGCTCTGGCCACACAC
  CCCAAGCATCAGGAGAGGTGCCGGGAGGAGATCCACAGCCTCCTGGGTGATGGAGCCTCC
  ATCACCTGGAACCACCTGGACCAGATGCCCTACACCACCATGTGCATTAAGGAGGCACTG
  AGGCTCTACCCACCGGTGCCAGGCATTGGMAGAGAGCTCAGCACTCCCGTCACCTTCCCT
  GATGGGCGCTCCTTGCCCAAAGGTATCATGGTCCTCCTCTCCATTTATGGCCTTCACCAC
  AACCCAAAAGTGTGGCCCAACCYAGAGGTGTTTGACCCTTTCCGTTTTGCACCGGGTTCT
  GCTCAACACAGCCACGCTTTCCTGCCCTTCTCAGGAGGATCAAGGAACTGCATCGGGAAA
  CAATTTGCCATGAACSAGCTGAAGGTGGCCASGGCCCTGACCCTGCTCCGCTTTGAGCTG
  CTGCCTGATCCCACCAGGATCCCCATCCCCATKGCACGACTTGTGTTGAAATCCAAAAAT
  GGAATCCACCTGCGTCTCAGGAGGCTCCCTAACCCTTGTGAAGACAAGGACCAGCTTTGA
  
  Clone variation with respect to NM_000778.3
  104 t=>y;120 c=>y;262 c=>y;268 c=>m;354 t=>w;362 g=>r;376 t=>y;598 t=>w;969 c=>t;1170 c=>m;1283 c=>y;1396 g=>s;1412 c=>s;1473 t=>k
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000778 unedited
  AGGAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCACTCAGA
  GATCCAGCAGGTGCTGCACCATGAGTGTCTCTGTGCTGAGCCCCAGCAGACTCCTGGGTG
  ATGTCTCTGGAATCCTCCAAGCGGCCTCCCTGCTCATTCTGCTTCTGCTGCTGATCAAGG
  CAGTTCAGCTCTACCTGCACAGGCAGTGGCTGCTCAAAGCCCTCCAGCAGTTCCCGTGCC
  CTCCCTCCCACTGGCTCTTCGGGCACATCCAGGAGCTCCAACAGGACCAGGAGCTACAAC
  GGATTCAGAAATGGGTGGAGACATTCCCAAGTGCCTGTCCTCATTGGCTATGGGGAGGCA
  AAGTTCGTGTCCAGCTCTATGACCCTGACTATATGAAGGTGATTCTGGGGAGATCAGACC
  CGAAATCCCATGGTTCCTACAGATTCCTGGCTCCATGGATTGGGTACGGCTTGCTCCTGT
  TGAATGGGCAGACATGGTTCCAGCATCGACGGATGCTGACCCCAGCCTTCCACTATGACA
  TCCTGAAGCCCTATGTGGGGCTCATGGCAGACTCTGTACGAGTGATGCTGGACAAATGGG
  AAGAGCTCCTTGGCCAGGATTCCCCTCTGGAGGTCTTTCAGCACGTCTCCTTGATGACCC
  TGGACACCATCATGAAGTGTGCCTTCAGCCATCAGGGCAGCATCCAGGTGGACAGGAATT
  CTCAGTCCTACATACAGGCCATTAGTGACCTGAACAACCTGNTTNNTTTCCCGTGTGAGG
  AATGCCTTTCACCAGAATGACACCATCTACAGCCTGACCTCTGCTGGCCGCTGGACACAC
  CGCGCCTGCCAGCTGGNCCATCAGCCACAGAACCAAGTGATCCCACTGAGGAAGCTCCCT
  TTACAGGAGGAGGGGGAGCTGNAGAAAATCAAAAGNANNAGCATNGNATTTCTGAATNCC
  TCTCTGCCAAAGNAGAATGGACATCTGTCGATAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000778 unedited
  CGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCT
  ATGATTTTGGCCGCAATCTAGAGTCGAGTTTTTTTTTGGGGTTTTTTTTTTTTTCTGGAA
  GTGCAGATTATTTATTGGTGATCAAACAAAGAAACAGGTGGTGAGAATGTGGGGGTCGAA
  AGGGCATGTTGCATTAACCACATGATTTACAGCTGTGACGGTTTAGCATCTGCTCTGCTA
  CTTGAGATAATGGAGAGCAGGTTCTTTTAACTCAAGATACAATTGATCCTGGGAGAGCAA
  GGAGCGAGGAGGCAGCAAGTCCAGACACATTCCAGAGCCACGAGCCCTGGATTCTATCCA
  AGCCACGAGGGGTTTTATGCCCCGGGCTTAGATTATGGTGCGTCAGGGTAGCCTTCCACC
  CTTCAGCACAGAGCTTGGTGTTCCAAAGGCCACAAGGGGTTTTAGACCCTGGATCCTGGA
  CATGATTCAAGACTCTTTTACATTATGTCAGACATGCAAGCTCGGCCTCAGCTTCTCCCA
  ACACTCAGCTTTTCTCCCAACAAGAGATACAGGTGGGTAGGAGACAGGTAGACAAGCAGG
  TAGGGAGCCTGGANAAAAGTGAGAGAGAGAAGGGCANGCAAACTGNNGGACAGCAGGCAG
  GTGGGAAGAGGGAAGGTGGGCAGACAGAAAACAGGATATGGGCAGACAGGAAGGGGACAG
  AAAGCGGGGTCAGGAAGACAGGACGGCANGTGGAGGCCCTCAAAGCTTGTCCTTGTCTTC
  ACAGGGGTTAGGGAGCCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000778
• Insert Size: 2200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000778.2, NP_000769.1
• RefSeq Size: 2815 bp
• RefSeq ORF: 1560 bp
• Locus ID: 1579
• UniProt ID: Q02928
• Cytogenetics: 1p33
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway, Retinol metabolism, Vascular smooth muscle contraction
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates medium-chain fatty acids such as laurate and myristate. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).