PTPRN2 (NM_002847) Human Untagged Clone

CAT#: SC120495

PTPRN2 (untagged)-Human protein tyrosine phosphatase, receptor type, N polypeptide 2 (PTPRN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPRN2
• Synonyms: IA-2beta; IAR; ICAAR; PTPRP; R-PTP-N2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120495 sequence for NM_002847 edited (data generated by NextGen Sequencing)
  ATGGGGCCGCCGCTCCCGCTGCTGCTGCTGCTACTGCTGCTGCTGCCGCCACGCGTCCTG
  CCTGCCGCCCCTTCGTCCGTCCCCCGCGGCCGGCAGCTCCCGGGGCGTCTGGGCTGCCTG
  CTCGAGGAGGGCCTCTGCGGAGCGTCCGAGGCCTGTGTGAACGATGGAGTGTTTGGAAGG
  TGCCAGAAGGTTCCGGCAATGGACTTTTACCGCTACGAGGTGTCGCCCGTGGCCCTGCAG
  CGCCTGCGCGTGGCGTTGCAGAAGCTTTCCGGCACAGGTTTCACGTGGCAGGATGACTAT
  ACTCAGTATGTGATGGACCAGGAACTTGCAGACCTCCCGAAAACCTACCTGAGGCGTCCT
  GAAGCATCCAGCCCAGCCAGGCCCTCAAAACACAGCGTTGGCAGCGAGAGGAGGTACAGT
  CGGGAGGGCGGTGCTGCCCTGGCCAACGCCCTCCGACGCCACCTGCCCTTCCTGGAGGCC
  CTGTCCCAGGCCCCAGCCTCAGACGTGCTCGCCAGGACCCATACGGCGCAGGACAGACCC
  CCCGCTGAGGGTGATGACCGCTTCTCCGAGAGCATCCTGACCTATGTGGCCCACACGTCT
  GCGCTGACCTACCCTCCCGGGCCCCGGACCCAGCTCCGCGAGGACCTCCTGCCGCGGACC
  CTCGGCCAGCTCCAGCCAGATGAGCTCAGCCCTAAGGTGGACAGTGGTGTGGACAGACAC
  CATCTGATGGCGGCCCTCAGTGCCTATGCTGCCCAGAGGCCCCCAGCTCCCCCCGGGGAG
  GGCAGCCTGGAGCCACAGTACCTTCTGCGTGCACCCTCAAGAATGCCCAGGCCTTTGCTG
  GCACCAGCCGCCCCCCAGAAGTGGCCTTCACCTCTGGGAGATTCCGAAGACCCCTCTAGC
  ACAGGCGATGGAGCACGGATTCATACCCTCCTGAAGGACCTGCAGAGGCAGCCGGCTGAG
  GTGAGGGGCCTGAATGGCCTGGAGCTGGACGGCATGGCCGAGCTGATGGCTGGCCTGATG
  CAAGGCGTGGACCATGGAGTAGCTCGAGGCAGCCCTGGGAGAGCGGCCCTGGGAGAGTCT
  GGAGAACAGGCGGATGGCCCCAAGGCCACCCTCCGTGGAGACAGCTTTCCAGATGACGGA
  GTGCAGGACGACGATGATAGACTTTACCAAGAGGTCCATCGTCTGAGTGCCACACTCGGG
  GGCCTCCTGCAGGACCACGGGTCTCGACTCTTACCTGGAGCCCTCCCCTTTGCAAGGCCC
  CTCGACATGGAGAGGAAGAAGTCCGAGCACCCTGAGTCTTCCCTGTCTTCAGAAGAGGAG
  ACTGCCGGAGTGGAGAACGTCAAGAGCCAGACGTATTCCAAAGATCTGCTGGGGCAGCAG
  CCGCATTCGGAGCCCGGGGCCGCTGCGTTTGGGGAGCTCCAAAACCAGATGCCTGGGCCC
  TCGAAGGAGGAGCAGAGCCTTCCAGCGGGTGCTCAGGAGGCCCTCAGCGACGGCCTGCAA
  TTGGAGGTCCAGCCTTCCGAGGAAGAGGCGCGGGGCTACATCGTGACAGACAGAGACCCC
  CTGCGCCCCGAGGAAGGAAGGCGGCTGGTGGAGGACGTCGCCCGCCTCCTGCAGGTGCCC
  AGCAGTGCGTTCGCTGACGTGGAGGTTCTCGGACCAGCAGTGACCTTCAAAGTGAGCGCC
  AATGTCCAAAACGTGACCACTGAGGATGTGGAGAAGGCCACAGTTGACAACAAAGACAAA
  CTGGAGGAAACCTCTGGACTGAAAATTCTTCAAACCGGAGTCGGGTCGAAAAGCAAACTC
  AAGTTCCTGCCTCCTCAGGCGGAGCAAGAAGACTCCACCAAGTTCATCGCGCTCACCCTG
  GTCTCCCTCGCCTGCATCCTGGGCGTCCTCCTGGCCTCTGGCCTCATCTACTGCCTCCGC
  CATAGCTCTCAGCACAGGCTGAAGGAGAAGCTCTCGGGACTAGGGGGCGACCCAGGTGCA
  GATGCCACTGCCGCCTACCAGGAGCTGTGCCGCCAGCGTATGGCCACGCGGCCACCAGAC
  CGACCTGAGGGCCCGCACACGTCACGCATCAGCAGCGTCTCATCCCAGTTCAGCGACGGG
  CCGATCCCCAGCCCCTCCGCACGCAGCAGCGCCTCATCCTGGTCCGAGGAGCCTGTGCAG
  TCCAACATGGACATCTCCACCGGCCACATGATCCTGTCCTACATGGAGGACCACCTGAAG
  AACAAGAACCGGCTGGAGAAGGAGTGGGAAGCGCTGTGCGCCTACCAGGCGGAGCCCAAC
  AGCTCGTTCGTGGCCCAGAGGGAGGAGAACGTGCCCAAGAACCGCTCCCTGGCCGTGCTG
  ACCTATGACCACTCCCGGGTCCTGCTGAAGGCGGAGAACAGCCACAGCCACTCAGACTAC
  ATCAACGCTAGCCCCATCATGGATCACGACCCGAGGAACCCCGCGTACATCGCCACCCAG
  GGACCGCTGCCCGCCACCGTGGCTGACTTTTGGCAGATGGTGTGGGAGAGCGGCTGCGTG
  GTGATCGTCATGCTGACACCCCTCGCGGAGAACGGCGTCCGGCAGTGCTACCACTACTGG
  CCGGATGAAGGCTCCAATCTCTACCACATCTATGAGGTGAACCTGGTCTCCGAGCACATC
  TGGTGTGAGGACTTCCTGGTGAGGAGCTTCTATCTGAAGAACCTGCAGACCAACGAGACG
  CGCACCGTGACGCAGTTCCACTTCCTGAGTTGGTATGACCGAGGAGTCCCTTCCTCCTCA
  AGGTCCCTCCTGGACTTCCGCAGAAAAGTAAACAAGTGCTACAGGGGCCGTTCTTGTCCA
  ATAATTGTTCATTGCAGTGACGGTGCAGGCCGGAGCGGCACCTACGTCCTGATCGACATG
  GTTCTCAACAAGATGGCCAAAGGTGCTAAAGAGATTGATATCGCAGCGACCCTGGAGCAC
  TTGAGGGACCAGAGACCCGGCATGGTCCAGACGAAGGAGCAGTTTGAGTTCGCGCTGACA
  GCCGTGGCTGAGGAGGTGAACGCCATCCTCAAGGCCCTTCCCCAGTGA
  
  Clone variation with respect to NM_002847.3
  622 t=>c;897 c=>t;974 g=>a;999 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002847 unedited
  GCCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTC
  ATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGG
  CACGAGGGAGGAGCCGGCGGCGCGCGCCTCCCTGGGCCCGGACTCGGCCGCCTCCCGCCG
  CCTCCCGCGCGGCCATGGACTGAGCGCCGCCGGCCAGGCCGCGGGGATGGGGCCGCCGCT
  CCCGCTGCTGCTGCTGCTACTGCTGCTGCTGCCGCCACGCGTCCTGCCTGCCGCCCCTTC
  GTCCGTCCCCCGCGGCCGGCAGCTCCCGGGGCGTCTGGGCTGCCTGCTCGAGGAGGGCCT
  CTGCGGAGCGTCCGAGGCCTGTGTGAACGATGGAGTGTTTGGAAGGTGCCAGAAGGTTCC
  GGCAATGGACTTTTACCGCTACGAGGTGTCGCCCGTGGCCCTGCAGCGCCTGCGCGTGGC
  GTTGCAGAAGCTTTCCGGCACAGGTTTCACGTGGCAGGATGACTATACTCAGTATGTGAT
  GGACCAGGAACTTGCAGACCTCCCGAAAACCTACCTGAGGCGTCCTGAAGCATCCAGCCC
  AGCCAGGCCCTCAAACACAGCGTTGGCAGCGAGAGGAGGTACAGTCGGGAGGGCGGTGCT
  GNCCTGGNNCAACGCCTNCGACGCCACCTGGCCTTTCTGGGAGCCCTGTNCCAGGCCCCA
  GCCTCAGACGTGCTCGCCAGGACCATACNGNGCANGACAGACCCCCGCCTGAGGTGATGA
  CCGCTCCTNCGAGAGCATCCTGACCTATGTGGNCCACACGTNTGCGCTGACCTACCCCTC
  CGGNCCCCGGACCAGCTCCGCGAGGACCCTCTGGCGGGNACCCTCGCCAGCTNCAGCCAG
  ATGAGCTCAGCCCTAGGTGGAACGGGGNNTGGGGACAGAACCCCATCTGATGGCGGCCCT
  CAGTGGCTATGCTGGCCAGAGGCCCCAGCTCCCGCGGGGGGAGGGCAAGCTGGAGGCCAC
  GAACCTNNTGGGTGCACCTNAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_002847 unedited
  ACTATGGTACCGCGGCCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGTGAAATC
  AGAGTTTAATATGACACAATTAAATATATTTGTATATCTCACACCGGAGGTTTCTCTTCA
  AACATAAGGAGTTAGAAATTACAAGTAGGCATATGCTTCCTATATTCAGATAAATTCATT
  TCGATTAATTAAATTCCAGATAGAGAGAAGTAATTTTGGAAAAGAAATGATAGCTATATT
  AAAGCAGATATTCATTACAATACCATGTAGAGACATAAGCAATATTTTGGCATCATTCTG
  TCCGCTCAGTAGGCCGTGTTCCCTCTGGTAGGGCCTTTGGAGAGTACCATCTATCTAAGA
  TGGAGGAATGCTGTGGGAAGGGCGGGATGGAGGTGCGTTTTCTACGCTGAACCCCACACA
  GGAAATCTGCAGCCCACACAGCTGCCTCTGCGCCGCCTTCCATGTGATCATCCTGGTCAA
  TGAAGTGAATTGTCCTATTTCTGGGGTAGGGGGTGATGGCGAATCGGATACTCAAATGTT
  ATTTTGTCAAATCCGAATCATCTCTACGTGCTTAGGTTTACATGATGTCAAAACACGTGG
  TTCATTAAGTGATCCCGGGAAGAGGGGCAGGGCCGGTCGGCTGGTTTCTCACTTCCCTTC
  CAGGCTCTACGCAGTGCGTGCAGGTCGCCCTGATCTCTCCCGGGAGGGGAATGCTCGCAG
  CCCTCTCCGAGNTGCCCTGATCTCCTTCTCTCCCAGAGGGAAATGGTCCCTCTCTGGGGC
  CCCACAGTCCCGGACGTGCCTGGAGAGCCAGCGGGGCCTGGCAGGGNTCGTGTGCTCCCG
  GGGACCCTCTAGCTTGCTCAAGCCCAGATTGTTCTTCCTGTTGTGGTATCTCACAGGTAG
  CTTCACGGGGCATCGTTTTCATACGGAAAAACATTATTTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002847
• Insert Size: 5500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002847.2, NP_002838.1
• RefSeq Size: 4767 bp
• RefSeq ORF: 3048 bp
• Locus ID: 5799
• UniProt ID: Q92932
• Cytogenetics: 7q36.3
• Domains: Y_phosphatase, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Type I diabetes mellitus
• Gene Summary: This gene encodes a protein with sequence similarity to receptor-like protein tyrosine phosphatases. However, tyrosine phosphatase activity has not been experimentally validated for this protein. Studies of the rat ortholog suggest that the encoded protein may instead function as a phosphatidylinositol phosphatase with the ability to dephosphorylate phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-diphosphate, and this function may be involved in the regulation of insulin secretion. This protein has been identified as an autoantigen in insulin-dependent diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (1) encodes isoform 1.