EDARADD (NM_080738) Human Untagged Clone

CAT#: SC120378

EDARADD (untagged)-Human EDAR-associated death domain (EDARADD), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDARADD
• Synonyms: ECTD11A; ECTD11B; ED3; EDA3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120378 sequence for NM_080738 edited (data generated by NextGen Sequencing)
  ATGGCTTCACCGGACGACCCTCTGCGCGCAGATCATATGGTAAAGGAACCAGTGGAAGAC
  ACAGACCCTAGCACTTTATCCTTTAATATGTCAGACAAATATCCCATTCAAGATACGGAA
  CTCCCTAAAGCTGAAGAATGTGATACAATTACTTTGAACTGCCCACGAAATTCAGATATG
  AAAAATCAGGGAGAAGAAAATGGCTTTCCAGATAGCACTGGAGATCCTCTTCCAGAGATC
  AGCAAGGACAACTCCTGCAAAGAAAACTGTACTTGTTCCTCCTGCTTGCTCCGGGCCCCC
  ACCATAAGTGACTTGCTCAATGATCAGGACTTACTAGATGTGATCAGGATAAAGCTGGAT
  CCGTGTCACCCAACGGTGAAAAACTGGAGGAATTTTGCAAGCAAATGGGGGATGTCCTAT
  GACGAATTGTGCTTCCTGGAGCAGAGGCCACAGAGCCCCACCTTGGAGTTCTTGCTCCGG
  AACAGTCAGAGGACGGTGGGCCAGCTGATGGAGCTCTGCAGGCTCTACCACAGGGCCGAC
  GTGGAGAAGGTTCTGCGCAGGTGGGTGGACGAGGAGTGGCCCAAGCGGGAGCGTGGAGAC
  CCCTCCAGGCACTTCTAG
  
  Clone variation with respect to NM_080738.3
  339 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080738 unedited
  TAGGCGGCCGCGACATCGGCACGACCCGGGACACTCGGGGCCCCGCCTTGCGTCCCAGCC
  CCGGGTCGCGGCACCCCGGCTCCCGCCCCGCGCCTCTGGAGGGAGGTACCCGAGGGACCG
  CGCAGCGAAGGGGCTTTGCTAATTGCCAAAGCAGGAAGTGAGCTCGTGGAAGAAGCGAAG
  GAGGAGAAGAAGAAAGGAGGGGAAGGCGGAGGAGGGCAGGGGCGCGCAGAGCCACGGTTT
  GCTCCAGGCGCGTCGGAACCGCAGGACTTTTCATCCCCGTGGTCCCACGGTCCTCCCGCG
  CCCCGGAGGCCTGCCAGCCCCGCTCGGACGCTCGTTTGCCCCTAACCCGCCGCCATGGCT
  TCACCGGACGACCCTCTGCGCGCAGATCATATGGTAAAGGAACCAGTGGAAGACACAGAC
  CCTAGCACTTTATCCTTTAATATGTCAGACAAATATCCCATTCAAGATACGGAACTCCCT
  AAAGCTGAAGAATGTGATACAATTACTTTGAACTGCCCACGAAATTCAGATATGAAAAAT
  CAGGGAGAAGAAAATGGCTTTCCAGATAGCACTGGAGATCCTCTTCCAGAGATCANNGCA
  GGACACTCCTGNCAAGAAACTGTACTNTGTCCTCCTGCTTTGCTCNGGCCCCCACCATAA
  GTGACTTGCTCAATGATCANGACTTACTAGATGTGATCANGATAAAGCTGGATCCGTGTC
  ACCNCACGGTGAAAAACTGGNAGAATTTTTGCAGCAATGGGGGATGTCCTATGACGAATT
  GTGCTTCCTGNAGCAGAGGCACAGACCCCCACTTGGGAGTTCTTTGCTCGGACAATCANA
  GGACGGTGGCCCAGCTGATGGAGCTCTGCAGGCTCTACACAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080738 unedited
  NNNNNGGGTACATCTAAGNAACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTTTT
  TTTTTCTGGGACGGACTCTCACTCTGTCTCCAGAACTGGTGTGCAGTGGCGCAATCTCGG
  CTCACTGCAACCTGCTTCTGTAGAAGTTCTAAGGAAGAGGTGCGAACTCCACGGCGGTGG
  GCAAGGGTAGCTAACTAGCAGCGGCCCCTACAAATGTTGCTCGGGGGTCTTGAGCTGCCG
  GAGTTGACACAAGGGGAGGGGTCTAATTAGCCACCAGGTGACTGAAGGGCTTGCCTGCCC
  ACAGCTTACTTGGCTGGGGGGTTTCTGAAGTTCCTGCCGGCAAACTTAGCCTTGCTGCCC
  AGCTCCTCTTCAATTCTGAGGAGCTGGTTGTACTTGGCCAAGCGCTCAGATCAGCAAGGG
  GCACCAGTCTTGAGCTGCCCAGGTCACAGCCCCACCACCAGGTCAGTGATGAAGGTATTT
  TCAGTCTCCCCAGAATGATGAGGCACCATGACACACCAACCATTGGCCTGGGCCAGCTTG
  CACGCCTGAAGGGACTCAGTCACAGAGCGAATCTGGTTCACTTTGAGCAGGAGGCAGTTG
  CACTTCTTCTCATTCACGGCCGAGGCTGTCCTCTTTGGGTTGGTCACTCTGAGATCATCC
  TCCACTACCTGGATTCCTGCACTGGCCGTGAACTTCTGCCAAGCTCCCCAGTCATCCTGG
  TCAAAGGGATCTTCAGTAGACACCACTGGGTAGTTTTTGATGAAGGACTTGTACAGGTCA
  GCCAGACCGTCAGGTGAGATGTACCTGGTGGGGGTCGTCGAGAAACTTGAATTCCCAGTC
  ATACTTTCCAGACCTGAAGAACTCGGAGGCCTCTACGTCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080738
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080738.2, NP_542776.1
• RefSeq Size: 2891 bp
• RefSeq ORF: 618 bp
• Locus ID: 128178
• UniProt ID: Q8WWZ3
• Cytogenetics: 1q42.3-q43
• Domains: enolase
• Protein Families: Druggable Genome
• Gene Summary: This gene was identified by its association with ectodermal dysplasia, a genetic disorder characterized by defective development of hair, teeth, and eccrine sweat glands. The protein encoded by this gene is a death domain-containing protein, and is found to interact with EDAR, a death domain receptor known to be required for the development of hair, teeth and other ectodermal derivatives. This protein and EDAR are coexpressed in epithelial cells during the formation of hair follicles and teeth. Through its interaction with EDAR, this protein acts as an adaptor, and links the receptor to downstream signaling pathways. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (B) differs in the 5' region compared to variant A. It encodes an isoform (B) that contains a distinct and shorter N-terminus, as compared to isoform (A).