SIRP alpha (SIRPA) (NM_080792) Human Untagged Clone

CAT#: SC120326

SIRPA (untagged)-Human signal-regulatory protein alpha (SIRPA), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIRP alpha
• Synonyms: BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS1; SIRP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_080792 edited
  GAATTCGGCACGAGGCCCTGGCTTTATTTCTCGCGCGCTTGGGGTCTCTCCCAGTCTCCG
  TCTCTCCATTTCTCCTGGGGGGCGGGGAGGGGGGGTCTCCAAAAACCGCGGCGGCGGCGG
  CGGCCGCTCCAGGCGCCCGTTCCGGAGTCGGGGGGAGGCCCAGCCGGGAGGGGGGAAGGG
  GGGGAGCCTTAGTCATTTCCCCGCTCCAGCCTGCTCCCGCCCGAGCGCGCACTCACGGCC
  GCTCTCCCTCCTCGCTCCGCAGCCGCGGCCCATGGAGCCCGCCGGCCCGGCCCCCGGCCG
  CCTCGGGCCGCTGCTCTGCCTGCTGCTCGCCGCGTCCTGCGCCTGGTCAGGAGTGGCGGG
  TGAGGAGGAGCTGCAGGTGATTCAGCCTGACAAGTCCGTGTTGGTTGCAGCTGGAGAGAC
  AGCCACTCTGCGCTGCACTGCGACCTCTCTGATCCCTGTGGGGCCCATCCAGTGGTTCAG
  AGGAGCTGGACCAGGCCGGGAATTAATCTACAATCAAAAAGAAGGCCACTTCCCCCGGGT
  AACAACTGTTTCAGACCTCACAAAGAGAAACAACATGGACTTTTCCATCCGCATCGGTAA
  CATCACCCCAGCAGATGCCGGCACCTACTACTGTGTGAAGTTCCGGAAAGGGAGCCCCGA
  TGACGTGGAGTTTAAGTCTGGAGCAGGCACTGAGCTGTCTGTGCGCGCCAAACCCTCTGC
  CCCCGTGGTATCGGGCCCTGCGGCGAGGGCCACACCTCAGCACACAGTGAGCTTCACCTG
  CGAGTCCCACGGCTTCTCACCCAGAGACATCACCCTGAAATGGTTCAAAAATGGGAATGA
  GCTCTCAGACTTCCAGACCAACGTGGACCCCGTAGGAGAGAGCGTGTCCTACAGCATCCA
  CAGCACAGCCAAGGTGGTGCTGACCCGCGAGGACGTTCACTCTCAAGTCATCTGCGAGGT
  GGCCCACGTCACCTTGCAGGGGGACCCTCTTCGTGGGACTGCCAACTTGTCTGAGACCAT
  CCGAGTTCCACCCACCTTGGAGGTTACTCAACAGCCCGTGAGGGCAGAGAACCAGGTGAA
  TGTCACCTGCCAGGTGAGGAAGTTCTACCCCCAGAGACTACAGCTGACCTGGTTGGAGAA
  TGGAAACGTGTCCCGGACAGAAACGGCCTCAACCGTTACAGAGAACAAGGATGGTACCTA
  CAACTGGATGAGCTGGCTCCTGGTGAATGTATCTGCCCACAGGGATGATGTGAAGCTCAC
  CTGCCAGGTGGAGCATGACGGGCAGCCAGCGGTCAGCAAAAGCCATGACCTGAAGGTCTC
  AGCCCACCCGAAGGAGCAGGGCTCAAATACCGCCGCTGAGAACACTGGATCTAATGAACG
  GAACATCTATATTGTGGTGGGTGTGGTGTGCACCTTGCTGGTGGCCCTACTGATGGCGGC
  CCTCTACCTCGTCCGAATCAGACAGAAGAAAGCCCAGGGCTCCACTTCTTCTACAAGGTT
  GCATGAGCCCGAGAAGAATGCCAGAGAAATAACACAGGACACAAATGATATCACATATGC
  AGACCTGAACCTGCCCAAGGGGAAGAAGCCTGCTCCCCAGGCTGCGGAGCCCAACAACCA
  CACGGAGTATGCCAGCATTCAGACCAGCCCGCAGCCCGCGTCGGAGGACACCCTCACCTA
  TGCTGACCTGGACATGGTCCACCTCAACCGGACCCCCAAGCAGCCGGCCCCCAAGCCTGA
  GCCGTCCTTCTCAGAGTACGCCAGCGTCCAGGTCCCGAGGAAGTGAATGGGACCGTGGTT
  TGCTCTAGCACCCATCTCTACGCGCTTTCTTGTCCCACAGGGAGCCGCCGTGATGAGCAC
  AGCCAACCCAGTTCCCGGAGGGCTGGGGCGGTGCAGGCTCTGGGACCCAGGGGCCAGGGT
  GGCTCTTCTCTCCCCACCCCTCCTTGGCTCTCCAGCACTTCCTGGGCAGCCACGGCCCCC
  TCCCCCCACATTGCCACATACCTGGAGGCTGACGTTGCCAAACCAGCCAGGGAACCAACC
  TGGGAAGTGGCCAGAACTTCCTGGGGTCCAAGAACTCTTGTGCCTCCGTCCATCACCATG
  TGGGTTTTGAAGACCCTCGACTGCCTCCCCGATGCTCCGAAGCCTGATCTTCCAGGGTGG
  GGAGGAGAAAATCCCACCTCCCCTGACCTCCACCACCTCCACCACCACCACCACCACCAC
  CACCACCACTACCACCACCACCCAACTGGGGCTAGAGTGGGGAAGATTTCCCCTTTAGAT
  CAAACTGCCCCTTCCATGGAAAAGCTGGAAAAAAACTCTGGAACCCATATCCAGGCTTGG
  TGAGGTTGCTGCCAACAGTCCTGGCCTCCCCCATCCCTAGGCTAAAGAGCCATGAGTCCT
  GGAGGAGGAGAGGACCCCTCCCAAAGGACTGGAGACAAAACCCTCTGCTTCCTTGGGTCC
  CTCCAAGACTCCCTGGGGCCCAACTGTGTTGCTCCACCCGGACCCATCTCTCCCTTCTAG
  ACCTGAGCTTGCCCCTCCAGCTAGCACTAAGCAACATCTCGCTGTGGACGCCTGTAAATT
  ACTGAGAAATGTGAAACGTGCAATCTTGAAACTGAGGTGTTAGAAAACTTGATCTGTGGT
  GTTTTGTTTTGTTTTTTTTCTTAAAACAACAGCAACGTGATCTTGGCTGTCTGTCATGTG
  TTGAAGTCCATGGTTGGGTCTTGTGAAGTCTGAGGTTTAACAGTTTGTTGTCCTGGAGGG
  ATTTTCTTACAGCGAAGACTTGAGTTCCTCCAAGTCCCAGAACCCCAAGAATGGGCAAGA
  AGGATCAGGTCAGCCACTCCCTGGAGACACAGCCTTCTGGCTGGGACTGACTTGGCCATG
  TTCTCAGCTGAGCCACGCGGCTGGTAGTGCAGCCTTCTGTGACCCCGCTGTGGTAAGTCC
  AGCCTGCCCAGGGCTGCTGAGGGCTGCCTCTTGACAGTGCAGTCTTATCGAGACCCAACG
  CCTCAGTCTGCTCATCCGTAAAGTGGGGATAGTGAAGATGACACCCCTCCCCACCACCTC
  TCATAAGCACTTTAGGAACACACAGAGGGTAGGGATAGTGGCCCTGGCCGTCTATCCTAC
  CCCTTTAGTGACCGCCCCCATCCCGGCTTTCTGAGCTGATCCTTGAAGAAGAAATCTTCC
  ATTTCTGCTCTCAAACCCTACTGGGATCAAACTGGAATAAATTGAAGACAGCCAGGGGGA
  TGGTGCAGCTGTGAAGCTCGGGCTGATTCCCCCTCTGTCCCAGAAGGTTGGCCAGAGGGT
  GTGACCCAGTTACCCTTTAACCCCCACCCTTCCAGTCGGGTGTGAGGGCCTGACCGGGCC
  CAGGGCAAGCAGATGTCGCAAGCCCTATTTATTCAGTCTTCACTATAACTCTTAGAGTTG
  AGACGCTAATGTTCATGACTCCTGGCCTTGGGATGCCCAAGGGATTTCTGGCTCAGGCTG
  TAAAAGTAGCTGAGCCATCCTGCCCATTCCTGGAGGTCCTACAGGTGAAACTGCAGGAGC
  TCAGCATAGACCCAGCTCTCTGGGGGATGGTCACCTGGTGATTTCAATGATGGCATCCAG
  GAATTAGCTGAGCCAACAGACCATGTGGACAGCTTTGGCCAGAGCTCCCGTGTGGCATCT
  GGGAGCCACAGTGACCCAGCCACCTGGCTCAGGCTAGTTCCAAATTCCAAAAGATTGGCT
  TGTAAACCTTCGTCTCCCTCTCTTTTACCCAGAGACAGCACATACGTGTGCACACGCATG
  CACACACACATTCAGTATTTTAAAAGAATGTTTTCTTGGTGCCATTTTCATTTTATTTTA
  TTTTTTAATTCTTGGAGGGGGAAATAAGGGAATAAGGCCAAGGAAGATGTATAGCTTTAG
  CTTTAGCCTGGCAACCTGGAGAATCCACATACCTTGTGTATTGAACCCCAGGAAAAGGAA
  GAGGTCGAACCAACCCTGCGGAAGGAGCATGGTTTCAGGAGTTTATTTTAAGACTGCTGG
  GAAGGAAACAGGCCCCATTTTGTATATAGTTGCAACTTAAACTTTTTGGCTTGCAAAATA
  TTTTTGTAATAAAGATTTCTGGGTAATAATGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080792 unedited
  AACTTTTGGATTTTGTAACACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCC
  TGGCTTTATTTCTCGCGCGCTTGGGGTCTCTCCCAGTCTCCGTCTCTCCATTTCTCCTGG
  GGGGCGGGGAGGGGGGGTCTCCAAAAACCGCGGCGGCGGCGGCGGCCGCTCCAGGCGCCC
  GTTCCGGAGTCGGGGGGAGGCCCAGCCGGGAGGGGGGAATGGGGGGAGCCTTATTCATTT
  CCCCGCTCCAGCCTGCTCCCGCCCGAGCGCGCACTCACGGCCGCTCTCCCTCCTCGCTCC
  GCAGCCGCGGCCCATGGAGCCCGCCGGCCCGGCCCCCGGCCGCCTCGGGCCGCTGCTCTG
  CCTGCTGCTCGCCGCGTCCTGCGCCTGGTCAGGAGTGGCGGGTGAGGAGGAGCTGCAGGT
  GATTCAGCCTGACAAGTCCGTGTTGGTTGCAGCTGGAGAGACAGCCACTCTGCGCTGCAC
  TGCGACCTCTCTGATCCCTGTGGGGCCCATCCAGTGGTTCAGAGGAGCTGGACCAGGCCG
  GGAATTAATCTACAATCAAAAAGAAAGCCACTTCCCCCGGGTAACAACTGTTTCAGACCT
  CACAAAGAGAAACAACATGGACTTTTCCATCCGCATCGGTAACATCACCCCAGCAGATGC
  CGGCACCTACTACTGTGTGAAGTTCCGGATAGGGAGCCCCGATGACGTGGAGTTTAAGTC
  TGGAGCAGGCACTGAGCTGTCTGTGCGCGCCAAACCCTCTGCCCCCGTGGTATCGGGCCC
  TGCGGCGAGGGCCACACCTCAGCACACAGTGAGCTTCACCTGCGAGTCCCACGGCTTCTC
  ACCAGAGACTCACCCTGAAATGGTTCANAAATGGGGATGAGCTCTCAGACTTNCAGACAA
  CGTGGACCCCCGTAGAGAGAGCGTGTCCTACAGCATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080792 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATTATTACCCAAAAAT
  CTTTATTACAAAAATATTTTGCAAGCCAAAAGTTTAAGTTGCAACTATATACAAAATGGG
  GCCTGTTTCCTTCCCAGCAGTCTTAAAATAAACTCCTGAAACCATGCTCCTTCCGCAGGG
  TTGGTTCGACCTCTTCCTTTTCCTGGGGTTCAATACACAAGGTATGTGGATTCTCCAGGT
  TGCCAGGCTAAAGCTAAAGCTATACATCTTCCTTGGCCTTATTCCCTTATTTCCCCCTCC
  AAGAATTAAAAAATAAAATAAAATGAAAATGGCACCAAGAAAACATTCTTTTAAAATACT
  GAATGTGTGTGTGCATGCGTGTGCACACGTATGTGCTGTCTCTGGGTAAAAGAGAGGGAG
  ACGAAGGTTTACAAGCCAATCTTTTGGAATTTGGAACTAGCCTGAGCCAGGTGGCTGGGT
  CACTGTGGCTCCCAGATGCCACACGGGAGCTCTGGCCAAAGCTGTCCACATGGTCTGTTG
  GCTCAGCTAATTCCTGGATGCCATCATTGAAATCACCAGGTGACCATCCCCCAGAGAGCT
  GGGTCTATGCTGAGCTCCTGCAGTTTCACCTGTAGGACCTCCAGGAATGGGCAGGATGGC
  TCAGCTACTTTTACAGCCTGAGCCAGAAATCCCTTGGGCATCCCAAGGCCAGGAGTCATG
  AACATTAGCGTCTCAACTCTAAGAGTATAGTGAAGACTGAAAAATAGGGCTTGCGACATC
  TGCTTGCCCTGGGCCCNGTCAGGCCCTCACACCCGACTGGAGGGTGGGGGGNTAAGGGGT
  ACTGGGTCACACCCTTGGCCACCTTCTGGACAGAGGGGAAATANCCGAGCTTACACTGCA
  CATCCCCTGGCTGNCTCAATTATTCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080792
• Insert Size: 3860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080792.1, NP_542970.1
• RefSeq Size: 3872 bp
• RefSeq ORF: 1515 bp
• Locus ID: 140885
• UniProt ID: P78324
• Cytogenetics: 20p13
• Domains: ig, IGv, IGc1, IG
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Gene Summary: The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate, in-frame, splice donor site in the 3' coding region compared to variant 4. This variant encodes a shorter protein (isoform 1) compared to isoform 2. Variants 1, 2, and 3 encode the same protein (isoform 1).