p15 INK4b (CDKN2B) (NM_078487) Human Untagged Clone

CAT#: SC120261

CDKN2B (untagged)-Human cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: p15 INK4b
• Synonyms: CDK4I; INK4B; MTS2; P15; p15INK4b; TP15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_078487, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGCGAGGAGAACAAGGGCATGCCCAGTGGGGGCGGCAGCGATGAGGGTCTGGCCAGCGCCGCGGCGC
  GGGGACTAGTGGAGAAGGTGCGACAGCTCCTGGAAGCCGGCGCGGATCCCAACGGAGTCAACCGTTTCGG
  GAGGCGCGCGATCCAGGTAGCTGGGGCCCCAGGGCCTCGCCGGCAGGGGGCGCGCGAACGCGGGGCGCGG
  CCTCGGCGGATCGGGGCTGGAACCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_078487 unedited
  CAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCCGGCTGGCTCC
  CCACTCTGCCAGAGCGAGGCGGGGCAGTGAGGACTCCGCGACGCGTCCGCACCCTGCGGC
  CAGAGCGGCTTTGAGCTCGGCTGCGTCCGCGCTAGGCGCTTTTTCCCAGAAGCAATCCAG
  GCGCGCCCGCTGGTTCTTGAGCGCCAGGAAAAGCCCGGAGCTAACGACCGGCCGCTCGGC
  CACTGCACGGGGCCCCAAGCCGCAGAAGGACGACGGGAGGGTAATGAAGCTGAGCCCAGG
  TCTCCTAGGAAGGAGAGAGTGCGCCGGAGCAGCGTGGGAAAGAAGGGAAGAGTGTCGTTA
  AGTTTACGGCCAACGGTGGATTATCCGGGCCGCTGCGCGTCTGGGGGCTGCGGAATGCGC
  GAGGAGAACAAGGGCATGCCCAGTGGGGGCGGCAGCGATGAGGGTCTGGCCAGCGCCGCG
  GCGCGGGGACTAGTGGAGAAGGTGCGACAGCTCCTGGAAGCCGGCGCGGATCCCAACGGA
  GTCAACCGTTTCGGGAGGCGCGCGATCCAGGTCATGATGATGGGCAGCGCCCGCGTGGCG
  GAGCTGCTGCTGCTCCACGGCGCGGAGCCCAACTGCGCAGACCCTGCCACTCTCACCCGA
  CCGGTGCATGATGCTGCCCGGGAGGGCTTCCTGGACACGCTGGTGGTGCTGCACCGGGCC
  GGGGCGCGGCTGGACGTGCGCGATGCCTGGGGTCGTCTGCCCGTGGACTTGGCCGAGGAG
  CGGGGCCACCGCGACCTTGCAGGTTACCTGCGCCAGCCACGGGGGACTGACGCCAAGTTT
  CCAAGCGCCACACGACTTTATTTTTTACCCATTTCCACCCCCACCACTTATTCATGAGGC
  TGCCACCGGGAGCGGCGGAAGCCTGTAGACTGCAACCTGTTTGGACTACAGAAGGGAGAG
  CGCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_078487 unedited
  AATACTGTAGTANCGCGGCCGCAATCGAGGGATCCGTTTTTTTTCTTTTTTTTTTTTTAA
  TGTTATTTTATTTATAAATTTATTTAACTTGCCCGATCTCTTGGAATAAATGTCAGGTAG
  TAGAATTTGTACAAACCTTGGTAATGTCTTAGGTTTAGATACGAACTTAACAGGTATATT
  TAATTTTCTGTATTCCACAATGGAGCTAGAAGCAGGACTCATGAAAATAGTAACTATATG
  TTTTGCAGGTATCCTTCAAAGTGAAATCCTTAACAAATTTAACAGTATATATTAGGCTGC
  TGATGAAACAGCTAAACCTGTCTGCCAGGTGGCTTCGAAAATGGAATTAATTTTTACATG
  GCATTGATAAGTTACTATTTCAATGCCACCGACTGGTGATTGATACCCAATAATGTTTGA
  GGTTTGCTTTACGGAAGAGAAACTGACAAAACAATCACAAAGAAATGTAATGTAGTACTG
  GGGGATCCAGAAAACATATTCTTTAGATTACCCGAAAAACAAGTGCCGTTAAGAAGAGTT
  CCAACATTTAATTAACACAAAAATAATGACATGTTCCAATATAGTTGATGGGAAAACATT
  CATGGCCCGGTTAGAGCGGACGGCACAGGAGGGGAACGGCCCGGCGCGGAAAAGCATACA
  CAATCAAGGAGGATATATAAACGTGGAACCTATTTGACAGTAGCAACGAATTAATTTTAT
  CACGATGGTCCCGCATGGTGCGAGGTACGGGGAGGCTGAATAGGGATGCTTGCGTGGTCA
  GGTACGGCGAGAGTGAAAGAATCGGCCCCCATGCTCTCCTGCTAATGCGCCAACGGTCGC
  GGGTATTTTGCCGCTTGAGCGTCTATCCATTGAGGGTCGCGCACCCGCCCGACGGCGACC
  ACTCCGCGCCGGGCCGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_078487
• Insert Size: 3890 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_078487.2, NP_511042.1
• RefSeq Size: 4001 bp
• RefSeq ORF: 237 bp
• Locus ID: 1030
• UniProt ID: P42772
• Cytogenetics: 9p21.3
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway
• Gene Summary: This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses a different splice site, which leads to a translation frame shift, when compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus when compared to isoform 1.