EXOSC6 (NM_058219) Human Untagged Clone

CAT#: SC120246

EXOSC6 (untagged)-Human exosome component 6 (EXOSC6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EXOSC6
• Synonyms: EAP4; hMtr3p; MTR3; Mtr3p; p11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_058219, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTGGGGATCACCGCCGCATCCGCGGCCCTGAAGAATCGCAGCCGCCGCAGCTGTACGCGGCCGACG
  AGGAGGAGGCGCCCGGCACCCGCGACCCAACGCGGCTACGGCCCGTGTACGCGCGCGCCGGGCTGCTGAG
  CCAGGCCAAGGGCTCGGCCTACCTGGAGGCGGGAGGCACCAAGGTGCTGTGTGCCGTGTCGGGCCCGCGA
  CAGGCCGAGGGCGGCGAGCGCGGCGGCGGCCCGGCCGGAGCAGGCGGCGAGGCCCCGGCCGCGCTGCGCG
  GTCGCCTGCTCTGCGACTTCCGCCGCGCACCCTTCGCGGGCCGCCGGCGCCGCGCTCCCCCGGGCGGCTG
  CGAGGAGCGTGAGCTGGCGCTGGCGCTGCAGGAGGCGCTGGAGCCGGCTGTGCGCCTGGGCCGCTACCCG
  CGCGCGCAGCTCGAGGTGTCGGCGCTGCTGCTGGAGGACGGTGGCTCGGCCCTGGCCGCCGCGCTCACCG
  CCGCCGCGCTCGCCCTGGCCGACGCGGGCGTGGAGATGTACGACCTGGTGGTGGGCTGCGGCCTCAGCCT
  CGCGCCGGGGCCCGCGCCCACCTGGCTCCTGGACCCCACGCGGCTCGAGGAAGAGCGCGCCGCCGCCGGC
  CTCACCGTGGCGCTCATGCCTGTGCTGAATCAGGTGGCCGGGCTGCTGGGCAGCGGCGAGGGCGGCCTGA
  CAGAGAGCTGGGCGGAGGCCGTACGCCTGGGCCTCGAGGGCTGCCAGCGCCTCTACCCCGTGCTGCAGCA
  GAGCCTGGTGCGGGCCGCCCGCCGCAGGGGCGCCGCCGCCCAGCCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_058219 unedited
  GGATTTTGTATACGACTTACTATAGGCGGCCGCGCATTCGGCACCANATGCCTGGGGATC
  ACCGCCGCATCCGCGGGCCCTTGAAAAATCGCAGCCGCCGCAGCTGTACGCGGCCGACGA
  GGAGGAGGCGCCCGGCACCCGCGACCCAACGCGGCTACGGCCCGTGTACGCGCGCGCCGG
  GCTGCTGAGCCAGGCCAAGGGCTCGGCCTACCTGGAGGCGGGAGGCACCAAGGTGCTGTG
  TGCCGTGTCGGGCCCGCGACAGGCCGAGGGCGGCGAGCGCGGCGGCGGCCCGGCCGGAGC
  AGGCGGCGAGGCCCCGGCCGCGCTGCGCGGTCGCCTGCTCTGCGACTTCCGCCGCGCACC
  CTTCGCGGGCCGCCGGCGCCGCGCTCCCCCGGGCGGCTGCGAGGAGCGTGAGCTGGCGCT
  GGCGCTGCAGGAGGCGCTGGAGCCGGCTGTGCGCCTGGGCCGCTACCCGCGCGCGCAGCT
  CGAGGTGTCGGCGCTGCTGCTGGAGGACGGTGGCTCGGCCCTGGCCGCCGCGCTCACCGC
  CGCCGCGCTCGCCCTGGCCGACGCGGGCGTGGAGATGTACGACCTGGTGGTGGGCTGCGG
  CCTCAGCCTCGCGCCGGGGCCCGCGCCCACCTGGCTCCTGGACCCCACGCGGCTCGAGGA
  AGAGCGCGCCGCCGCCGGCCTCACCGTGGCGCTCATGCCTGTGCTGAATCANGTGGCCGG
  GCTGCTGGGCAGNCGCGAGGGCGGCCTGACAGAGAGCTTGGCGGAGGCCGTACGCCTGGG
  CCTCGAGGGCTGCCAGCGCCTCTACCCCGTGCTGCAGCAGAGCCTGTTGCGGGCCCGCCG
  CCGCAAGGGCGCCCGCGCCCAGCCCGAACCAGAAGGCTGAGCACTACGGAACCAAGCGAG
  GACCGTGCTGCCGCTTCACAAAAGACCGCGCTTCGCCTCCATTTGCGTCAGAATCCTGGA
  GGGCCTGATTGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_058219
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_058219.2, NP_478126.1
• RefSeq Size: 1729 bp
• RefSeq ORF: 819 bp
• Locus ID: 118460
• UniProt ID: Q5RKV6
• Cytogenetics: 16q22.1
• Domains: RNase_PH_C
• Protein Pathways: RNA degradation
• Gene Summary: This gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. The composition of human exosome is similar to its yeast counterpart. This protein is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation. [provided by RefSeq, Jul 2008]