ALAS1 (NM_000688) Human Untagged Clone

CAT#: SC119768

ALAS1 (untagged)-Human aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ALAS1
• Synonyms: ALAS; ALAS-H; ALAS3; ALASH; MIG4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119768 sequence for NM_000688 edited (data generated by NextGen Sequencing)
  ATGGAGAGTGTTGTTCGCCGCTGCCCATTCTTATCCCGAGTCCCCCAGGCCTTTCTGCAG
  AAAGCAGGCAAATCTCTGTTGTTCTATGCCCAAAACTGCCCCAAGATGATGGAAGTTGGG
  GCCAAGCCAGCCCCTCGGGCATTGTCCACTGCAGCAGTACACTACCAACAGATCAAAGAA
  ACCCCTCCGGCCAGTGAGAAAGACAAAACTGCTAAGGCCAAGGTCCAACAGACTCCTGAT
  GGATCCCAGCAGAGTCCAGATGGCACACAGCTTCCGTCTGGACACCCCTTGCCTGCCACA
  AGCCAGGGCACTGCAAGCAAATGCCCTTTCCTGGCAGCACAGATGAATCAGAGAGGCAGC
  AGTGTCTTCTGCAAAGCCAGTCTTGAGCTTCAGGAGGATGTGCAGGAAATGAATGCCGTG
  AGGAAAGAGGTTGCTGAAACCTCAGCAGGCCCCAGTGTGGTTAGTGTGAAAACCGATGGA
  GGGGATCCCAGTGGACTGCTGAAGAACTTCCAGGACATCATGCAAAAGCAAAGACCAGAA
  AGAGTGTCTCATCTTCTTCAAGATAACTTGCCAAAATCTGTTTCCACTTTTCAGTATGAT
  CGTTTCTTTGAGAAAAAAATTGATGAGAAAAAGAATGACCACACCTATCGAGTTTTTAAA
  ACTGTGAACCGGCGAGCACACATCTTCCCCATGGCAGATGACTATTCAGACTCCCTCATC
  ACCAAAAAGCAAGTGTCAGTCTGGTGCAGTAATGACTACCTAGGAATGAGTCGCCACCCA
  CGGGTGTGTGGGGCAGTTATGGACACTTTGAAACAACATGGTGCTGGGGCAGGTGGTACT
  AGAAATATTTCTGGAACTAGTAAATTCCATGTGGACTTAGAGCGGGAGCTGGCAGACCTC
  CATGGGAAAGATGCCGCACTCTTGTTTTCCTCGTGCTTTGTGGCCAATGACTCAACCCTC
  TTCACCCTGGCTAAGATGATGCCAGGCTGTGAGATTTACTCTGATTCTGGGAACCATGCC
  TCCATGATCCAAGGGATTCGAAACAGCCGAGTGCCAAAGTACATCTTCCGCCACAATGAT
  GTCAGCCACCTCAGAGAACTGCTGCAAAGATCTGACCCCTCAGTCCCCAAGATTGTGGCA
  TTTGAAACTGTCCATTCAATGGATGGGGCGGTGTGCCCACTGGAAGAGCTGTGTGATGTG
  GCCCATGAGTTTGGAGCAATCACCTTCGTGGATGAGGTCCACGCAGTGGGGCTTTATGGG
  GCTCGAGGCGGAGGGATTGGGGATCGGGATGGAGTCATGCCAAAAATGGACATCATTTCT
  GGAACACTTGGCAAAGCCTTTGGTTGTGTTGGAGGGTACATCGCCAGCACGAGTTCTCTG
  ATTGACACCGTACGGTCCTATGCTGCTGGCTTCATCTTCACCACCTCTCTGCCACCCATG
  CTGCTGGCTGGAGCCCTGGAGTCTGTGCGGATCCTGAAGAGCGCTGAGGGACGGGTGCTT
  CGCCGCCAGCACCAGCGCAACGTCAAACTCATGAGACAGATGCTAATGGATGCCGGCCTC
  CCTGTTGTCCACTGCCCCAGCCACATCATCCCTGTGCGGGTTGCAGATGCTGCTAAAAAC
  ACAGAAGTCTGTGATGAACTAATGAGCAGACATAACATCTACGTGCAAGCAATCAATTAC
  CCTACGGTGCCCCGGGGAGAAGAGCTCCTACGGATTGCCCCCACCCCTCACCACACACCC
  CAGATGATGAACTACTTCCTTGAGAATCTGCTAGTCACATGGAAGCAAGTGGGGCTGGAA
  CTGAAGCCTCATTCCTCAGCTGAGTGCAACTTCTGCAGGAGGCCACTGCATTTTGAAGTG
  ATGAGTGAAAGAGAGAAGTCCTATTTCTCAGGCTTGAGCAAGTTGGTATCTGCTCAGGCC
  TGA
  
  Clone variation with respect to NM_000688.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000688 unedited
  GTATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGCGGCCTGAGGCT
  GCTCCCGGACAAGGGCAACGAGCGTTTCGTTTGGACTTCTCGACTTGAGTGCCCGCCTCC
  TTCGCCGCCGCCTCTGCAGTCCTCAGCGCAGTCTTTCCACAGGAGCCAGCATACTTCCTG
  AACATGGAGAGTGTTGTTCGCCGCTGCCCATTCTTATCCCGAGTCCCCCAGGCCTTTCTG
  CAGAAAGCAGGCAAATCTCTGTTGTTCTATGCCCAAAACTGCCCCAAGATGATGGAAGTT
  GGGGCCAAGCCAGCCCCTCGGGCATTGTCCACTGCAGCAGTACACTACCAACAGATCAAA
  GAAACCCCTCCGGCCAGTGAGAAAGACAAAACTGCTAAGGCCAAGGTCCAACAGACTCCT
  GATGGATCCCAGCAGAGTCCAGATGGCACACAGCTTCCGTCTGGACACCCCTTGCCTGCC
  ACAAGCCAGGGCACTGCAAGCAAATGCCCTTTCCTGGCAGCACAGATGAATCAGAGAGGC
  AGCAGTGTCTTCTGCAAAGCCAGTCTTGAGCTTCAGGAGGATGTGCAGGAAATGAATGCC
  GTGAGGAAAGAGGTTGCTGAAACCTCAGCAGGCCCCAGTGTGGTTAGTGTGAAAACCGAT
  GGAGGGGATCCCAGTGGACTGCTGAAGAACTTCCAGGACATCATGCAAAAGCANAGACCA
  GANAGAGTGTCTCATCTTCTTCAAGATACTTGCCAAATCTGTTTCCACTTTCAGTATGAT
  CGTTTCTTTGAGACAAAAATGATGAGAAAAAGATGACCCACCTATCGAGATCTTAAACTG
  TGACCTGGCGAGCCACATCTTCCCCTGCCAATGATATTCAGACTCCTCACACCAAAAGCA
  GTGTCAGTCTGTTGCAGTATGACTACCTAGAATGAGTCGCCACCCAGGGTGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000688 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTATTTCCAGGACTATGTTTT
  TACTATAGATTAAAATTTAATATAACTTAATTCACATATAAAGACAACTCTGAAGACCAT
  CTGGATATGATAATGGCCTGGGGTTAAGTGAAATAATTGAGGTCATGCTCAGGCCTGAGC
  AGATACCAACTTGCTCAAGCCTGAGAAATAGGACTTCTCTCTTTCACTCATCACTTCAAA
  ATGCAGTGGCCTCCTGCAGAAGTTGCACTCAGCTGAGGAATGAGGCTTCAGTTCCAGCCC
  CACTTGCTTCCATGTGACTAGCAGATTCTCAAGGAAGTAGTTCATCATCTGGGGTGTGTG
  GTGAGGGGTGGGGGCAATCCGTAGGAGCTCTTCTCCCCGGGGCACCGTAGGGTAATTGAT
  TGCTTGCACGTAGATGTTATGTCTGCTCATTAGTTCATCACAGACTTCTGTGTTTTTAGC
  AGCATCTGCAACCCGCACAGGGATGATGTGGCTGGGGCAGTGGACAACAGGGAGGCCGGC
  ATCCATTAGCATCTGTCTCATGAGTTTGACGTTGCGCTGGTGCTGGCGGCGAAGCACCCG
  TCCCTCAGCGCTCTTCAGGATCCGCACAGACTCCAGGGCTCCAGCCAGCAGCATGGGTGG
  CAGAGAGGTGGTGAAGATGAAGCCAGCAGCATAGGACCGTACGGTGTCAATCAGAGAACT
  CGTGCTGGCGATGTACCCTCCACACACCAAGGCTNTTGCCAGTGTCCAGAATGATGTCCA
  TTTTTGCATGACTCATCCCGATCCCATCCCCTCGCTCGGCCCATAAGCCCACTGCGTGAC
  CTATCACGAGGTGANTGCTCAACTATGGCCAATACACAGTTTTCGGGGCCACCGCCTCTT
  GATGGACGTTCATGCCATCTGGACGAGGGCAACTTTCGAGTTTGAGGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000688
• Insert Size: 2290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000688.4, NP_000679.1
• RefSeq Size: 2407 bp
• RefSeq ORF: 1923 bp
• Locus ID: 211
• UniProt ID: P13196
• Cytogenetics: 3p21.2
• Domains: ALA_synthase, aminotran_1_2
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism
• Gene Summary: This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein.