GRIA4 (NM_000829) Human Untagged Clone

CAT#: SC119639

GRIA4 (untagged)-Human glutamate receptor, ionotrophic, AMPA 4 (GRIA4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRIA4
• Synonyms: GluA4; GluA4-ATD; GLUR4; GLUR4C; GLURD; NEDSGA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000829, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGATTATTTCCAGACAGATTGTCTTGTTATTTTCTGGATTTTGGGGACTCGCCATGGGAGCCTTTC
  CGAGCAGCGTGCAAATAGGTGGTCTCTTCATCCGAAACACAGATCAGGAATACACTGCTTTTCGATTAGC
  AATTTTTCTTCATAACACCAGCCCCAATGCGTCGGAAGCTCCTTTTAATTTGGTACCTCATGTGGACAAC
  ATTGAGACAGCCAACAGTTTTGCTGTAACAAACGCCTTCTGTTCCCAGTATTCTAGAGGAGTATTTGCCA
  TTTTTGGACTCTATGATAAGAGGTCGGTACATACCTTGACCTCATTCTGCAGCGCCTTACATATCTCCCT
  CATCACACCAAGTTTCCCTACTGAGGGGGAGAGCCAGTTTGTGCTGCAACTAAGACCTTCGTTACGAGGA
  GCACTCTTGAGTTTGCTGGATCACTACGAATGGAACTGTTTTGTCTTCCTGTATGACACAGACAGGGGAT
  ACTCGATACTCCAAGCTATTATGGAAAAAGCAGGACAAAATGGTTGGCATGTCAGCGCTATATGTGTGGA
  AAATTTTAATGATGTCAGCTATAGGCAACTTCTAGAAGAACTTGACAGAAGACAAGAGAAGAAGTTTGTA
  ATAGACTGTGAGATAGAGAGACTTCAAAACATATTAGAACAGATTGTAAGTGTTGGAAAGCATGTTAAAG
  GCTACCATTATATCATTGCAAACTTGGGATTCAAGGATATTTCTCTTGAGAGGTTTATACATGGTGGAGC
  CAATGTTACTGGATTCCAGTTGGTGGATTTTAATACACCTATGGTAATCAAACTAATGGATCGCTGGAAG
  AAACTAGATCAGAGAGAGTATCCAGGATCTGAGACTCCTCCAAAGTACACCTCTGCTCTGACTTATGATG
  GAGTCCTTGTGATGGCTGAAACTTTCCGAAGTCTTAGGAGGCAGAAAATTGATATCTCAAGGAGAGGAAA
  TGCTGGGGATTGTCTGGCAAATCCTGCTGCTCCATGGGGCCAGGGAATTGACATGGAGAGGACACTCAAA
  CAGGTTCGAATTCAAGGGCTGACAGGGAATGTTCAGTTTGACCACTATGGACGTAGAGTCAATTACACAA
  TGGATGTGTTTGAGCTGAAAAGCACAGGACCTAGAAAGGTTGGTTACTGGAATGATATGGATAAGTTAGT
  CTTGATTCAAGATGTACCAACTCTTGGCAATGACACAGCTGCTATTGAGAACAGAACAGTGGTTGTAACC
  ACAATTATGGAATCCCCATATGTTATGTACAAGAAAAATCATGAAATGTTTGAAGGAAATGACAAGTATG
  AAGGATACTGTGTAGATTTGGCATCTGAAATTGCAAAACATATTGGTATCAAGTATAAAATTGCCATTGT
  CCCTGATGGAAAATATGGAGCAAGGGATGCAGACACAAAAATCTGGAATGGGATGGTAGGAGAACTTGTT
  TATGGGAAAGCAGAGATTGCTATTGCCCCTCTGACAATCACTTTGGTACGAGAGGAGGTCATTGACTTTT
  CTAAGCCCTTCATGAGTTTGGGCATATCTATCATGATCAAAAAGCCTCAGAAATCCAAACCAGGAGTGTT
  TTCCTTCTTGGATCCTCTGGCCTATGAGATTTGGATGTGCATAGTCTTTGCCTACATTGGTGTCAGCGTG
  GTCTTATTCCTAGTTAGTAGATTTAGTCCATATGAGTGGCACACAGAAGAGCCAGAGGACGGAAAGGAAG
  GACCCAGCGACCAGCCTCCCAATGAGTTTGGCATCTTTAACAGCCTCTGGTTTTCCCTGGGTGCTTTTAT
  GCAGCAAGGATGTGACATTTCACCCAGATCCCTCTCAGGTCGAATTGTTGGAGGTGTTTGGTGGTTCTTT
  ACACTCATCATTATATCATCTTATACTGCTAACCTCGCTGCTTTCCTGACGGTTGAGCGAATGGTCTCTC
  CCATAGAAAGTGCAGAAGACCTGGCCAAACAAACAGAAATTGCCTATGGAACACTGGATTCAGGATCAAC
  AAAAGAATTCTTCAGAAGATCAAAAATAGCAGTGTATGAAAAGATGTGGACCTACATGCGATCAGCAGAG
  CCATCAGTATTCACTAGGACTACAGCTGAGGGAGTAGCTCGTGTCCGCAAATCCAAGGGCAAATTTGCCT
  TTCTCCTGGAGTCCACTATGAATGAATACATTGAGCAGCGAAAGCCATGTGACACGATGAAAGTGGGAGG
  AAATCTGGATTCCAAAGGCTATGGAGTAGCAACGCCCAAGGGTTCCTCATTAGGAACTCCTGTAAACCTT
  GCCGTTTTGAAACTCAGTGAGGCAGGCGTCTTAGACAAGCTGAAAAACAAATGGTGGTACGATAAAGGTG
  AATGTGGACCCAAGGACTCTGGAAGCAAGGACAAGACGAGTGCCTTGAGCCTGAGCAATGTAGCAGGCGT
  CTTCTACATTCTGGTTGGCGGCTTGGGCTTGGCAATGCTGGTGGCTTTGATAGAGTTCTGTTACAAGTCC
  AGGGCAGAAGCGAAGAGAATGAAGCTGACCTTTTCTGAAGCCATAAGAAACAAAGCCAGATTATCCATCA
  CTGGGAGTGTGGGAGAGAATGGCCGCGTCTTGACGCCTGACTGCCCAAAGGCTGTACACACTGGAACTGC
  AATCAGACAAAGTTCAGGATTGGCTGTCATTGCATCGGACCTACCATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000829 unedited
  CGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAAACAAAGAGAGCGCGCGCCAGG
  NAGAGGAGAAAAGAAGATGAGGATTATTTCCAGACAGATTGTCTTGTTATTTTCTGGATT
  TTGGGGACTCGCCATGGGAGCCTTTCCGAGCAGCGTGCAAATAGGTGGTCTCTTCATCCG
  AAACACAGATCAGGAATACACTGCTTTTCGATTAGCAATTTTTCTTCATAACACCAGCCC
  CAATGCGTCGGAAGCTCCTTTTAATTTGGTACCTCATGTGGACAACATTGAGACAGCCAA
  CAGTTTTGCTGTAACAAACGCCTTCTGTTCCCAGTATTCTAGAGGAGTATTTGCCATTTT
  TGGACTCTATGATAAGAGGTCGGTACATACCTTGACCTCATTCTGCAGCGCCTTACATAT
  CTCCCTCATCACACCAAGTTTCCCTACTGAGGGGGAGAGCCAGTTTGTGCTGCAACTAAG
  ACCTTCGTTACGAGGAGCACTCTTGAGTTTGCTGGATCACTACGAATGGAACTGTTTTGT
  CTTCCTGTATGACACAGACAGGGGATACTCGATACTCCAAGCTATTATGGAAAAAGCAGG
  ACANAATGGTTGGCATGTCAGCGCTATATGTGTGGAAAATTNTAATGATGTCAGCTATAG
  GCAACTTCTAGAAGAACTTGACAGAAGACAAGAGAAGAAGTTTGTAATAGACTGTGAGAT
  AGAGAGACTTCANACATATTANAACAGATTGTAGTGTTGGAAAGCATGTTTAAGGCTACC
  ATTATATCATTGCANACTTGGGATTCAAGGATATTTCTCTGAGAGGTTATACATGGTGGA
  GCCAATGGTACTGGGATCCAGTTGGTGGATTTTAAACACCTAGGGAAACCAACTAAGGGA
  ACGCTGGAAGAAACTGATCAGAAGAGTTTCCGGATCTGAGACTCCTCCCAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000829 unedited
  CCCCCTACTTAGTCCGCGGCCGCAATCTACAGCGGGTTTTTTTTTTTTTTTTTATTGCAA
  GAACATCTGCTGTTTTATTCTTTTTGGTGGGTACAAAAACCACAATGTTGTACACAGAAT
  GAGATTATAAATAATACCATTAACATGAGTGACCTGTCATCTCTTTCGAGTCTACACCGC
  AGACTGATGATTGAACCTAAACGTTTTCTTCCTTTTGCTTTTCTTCCTGGTTGTGAGACA
  AATGCAATCGGTGAATCGACTCATGTGACATAGTGAAGGTTACAGCAAACAAGAAGATGA
  CCAACAACTTTTATAAAAAAAAGTCTCTAAAGTTTATTACAGTGATGGCATTTATATCAA
  TTATTGCAATGTATTGTTCCCTAAGATGGTTAGAAAAAAGAATTGTGTTGTCCTCTACTG
  AATTTACGTCAGTTAAAAAGCAAGCATGTTTCAGTATTTGTTTTCTCCAAAGTAAGTGTA
  CATACTTCCTTTATTTTTTTTTTCCCACAGGTTTTACATGTCTGCTCATTATTTTCTTAC
  CCCACCTACCGCTCCCCCCCCCTCCCTCCCACCATCCCCCCCCCGCCATCCCGACCACCC
  CCCCCCCCCTTTCCCTCCTACCTCTCCCACAACCCTCATATTCCCCTCCTCCCCCCCCCC
  CCCCCTTCCTCCCCTCCCTTCTCTTCTCCCCCCCCCTCTCCCCCCCCCACCTCCCTCCCA
  CCCCCCCCCCCCCCCCCCTCCACCCTCCAACCCTCCCCCCCCTCCCCCCCCCCTCTCTCT
  CTTTTCTTTCATCCCCTACCTTCACTACCCCCACACCCCACCTCACTCCCCCCACCCACC
  CTACCACATCCCCCACCCCTTCCTTCCTTCCCCCCCCAACTCACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000829
• Insert Size: 4300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000829.1, NP_000820.1
• RefSeq Size: 5508 bp
• RefSeq ORF: 5508 bp
• Locus ID: 2893
• UniProt ID: P48058
• Cytogenetics: 11q22.3
• Domains: lig_chan, ANF_receptor
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (1). RNA editing (AGA->GGA) changes Arg765Gly. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.