RPL12 (NM_000976) Human Untagged Clone

CAT#: SC119578

RPL12 (untagged)-Human ribosomal protein L12 (RPL12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL12
• Synonyms: L12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119578 sequence for NM_000976 edited (data generated by NextGen Sequencing)
  ATGCCGCCGAAGTTCGACCCCAACGAGATCAAAGTCGTATACCTGAGGTGCACCGGAGGT
  GAAGTCGGTGCCACTTCTGCCCTGGCCCCCAAGATCGGCCCCCTGGGTCTGTCTCCAAAA
  AAAGTTGGTGATGACATTGCCAAGGCAACGGGTGACTGGAAGGGCCTGAGGATTACAGTG
  AAACTGACCATTCAGAACAGACAGGCCCAGATTGAGGTGGTGCCTTCTGCCTCTGCCCTG
  ATCATCAAAGCCCTCAAGGAACCACCAAGAGACAGAAAGAAACAGAAAAACATTAAACAC
  AGTGGGAATATCACTTTTGATGAGATTGTCAACATTGCTCGACAGATGCGGCACCGATCC
  TTAGCCAGAGAACTCTCTGGAACCATTAAAGAGATCCTGGGGACTGCCCAGTCAGTGGGC
  TGTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACATCAACAGTGGTGCTGTG
  GAATGCCCAGCCAGTTAA
  
  Clone variation with respect to NM_000976.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000976 unedited
  CACGAGGCCAAGGTGCAACTTCCTTCGGTCGTCCCGAATCCGGGTTCATCCGACACCAGC
  CGCCTCCACCATGCCGCCGAAGTTCGACCCCAACGAGATCAAAGTCGTATACCTGAGGTG
  CACCGGAGGTGAAGTCGGTGCCACTTCTGCCCTGGCCCCCAAGATCGGCCCCCTGGGTCT
  GTCTCCAAAAAAAGTTGGTGATGACATTGCCAAGGCAACGGGTGACTGGAAGGGCCTGAG
  GATTACAGTGAAACTGACCATTCAGAACAGACAGGCCCAGATTGAGGTGGTGCCTTCTGC
  CTCTGCCCTGATCATCAAAGCCCTCAAGGAACCACCAAGAGACAGAAAGAAACAGAAAAA
  CATTAAACACAGTGGGAATATCACTTTTGATGAGATTGTCAACATTGCTCGACAGATGCG
  GCACCGATCCTTAGCCAGAGAACTCTCTGGAACCATTAAAGAGATCCTGGGGACTGCCCA
  GTCAGTGGGCTGTAATGTTGATGGCCGCCATCCTCATGACATCATCGATGACATAACAGT
  GGGTGCTTGTGGAATGCCCAGCCAGTTAAGCACAAAGGAAAACATTTAATAAAGGATCAT
  TTGACAAAAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000976 unedited
  NTTTAGCTTGNCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTCAAAT
  GATCCTTTATTGAAATGTTTTCCTTTGTGCTTAACTGGCTGGGCATTCCACAGCACCACT
  GTTGATGTCATCGATGATGTCATGAGGATGGCGGCCATCAACATTACAGCCCACTGACTG
  GGCAGTCCCCAGGATCTCTTTAATGGTTCCAGAGAGTTCTCTGGCTAAGGATCGGTGCCG
  CATCTGTCGAGCAATGTTGACAATCTCATCAAAAGTGATATTCCCACTGTGTTTAATGTT
  TTTCTGTTTCTTTCTGTCTCTTGGTGGTTCCTTGAGGGCTTTGATGATCAGGGCAGAGGC
  AGAAGGCACCACCTCAATCTGGGCCTGTCTGTTCTGAATGGTCAGTTTCACTGTAATCCT
  CAGGCCCTTCCAGTCACCCGTTGCCTTGGCAATGTCATCACCAACTTTTTTTGGAGACAG
  ACCCAGGGGGCCGATCTTGGGGGCCAGGGCAGAAGTGGCACCGACTTCACCTCCGGTGCA
  CCTCAGGTATACGACTTTGATCTCGTTGGGGTCGAACTTCGGCGGCATGGTGGAGGCGGC
  TGGTGTCGGATGAACCCGGATTCGGGACGACCGAAGGAAGTTGCACCTTGGCCTCGTGCC
  GAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAG
  CTCTGCTTATATAGACCTNCCACCGTACACGCCTACCGNCCATTTGCGTCAACGGGCGGG
  GTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAACANACTNCCATTGACG
  TCATGGGGTGGAGACTTGGAAATCCCGTGAGTCAACCGCTTCCACCCCATTGGTTACTGC
  CAAAACGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000976
• Insert Size: 1920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000976.2, NP_000967.1
• RefSeq Size: 632 bp
• RefSeq ORF: 498 bp
• Locus ID: 6136
• UniProt ID: P30050
• Cytogenetics: 9q33.3
• Domains: Ribosomal_L11
• Protein Pathways: Ribosome
• Gene Summary: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]